Search results for the GEO ID: GSE20689  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM519449 | GPL570 |
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SW620_MOCK_KO
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Sw620 human colorectal cancer cell line with Mock knockdown
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cell line: Sw620 colorectal cancer
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gene expression data from liver of ECM1 Ko mice
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| Sample_geo_accession | GSM519449
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sox2 was knockdown in sw620 cell lines by lentivrus mediated ShRNA and mock no target conrtol ShRNA
| | Sample_growth_protocol_ch1 | cells were cultured in RPMI-1640 cell culture media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit,(Lot no. 901228) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | the chips were scanned withthe affymetrix genechip 7G plus, and AGCC is used for the image processing.
| | Sample_data_processing | RMA normalization using GeneSpring 7.3
| | Sample_platform_id | GPL570
| | Sample_contact_name | Biaoyang,,Lin
| | Sample_contact_email | bylin@u.washington.edu
| | Sample_contact_phone | 206 320 3570
| | Sample_contact_fax | 206 320 31 66
| | Sample_contact_department | Swedish Neuroscience Institute
| | Sample_contact_institute | Swedish Medical Center
| | Sample_contact_address | 550 17th Ave, James Tower suite 550
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98122
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519449/suppl/GSM519449.CEL.gz
| | Sample_series_id | GSE20689
| | Sample_data_row_count | 54675
| | |
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GSM519450 | GPL570 |
|
SW620_SOX2_KO
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Sw620 human colorectal cancer cell line with Sox2 knockdown
|
cell line: Sw620 colorectal cancer
|
gene expression data from liver of ECM1 Ko mice
|
| Sample_geo_accession | GSM519450
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sox2 was knockdown in sw620 cell lines by lentivrus mediated ShRNA and mock no target conrtol ShRNA
| | Sample_growth_protocol_ch1 | cells were cultured in RPMI-1640 cell culture media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit,(Lot no. 901228) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | the chips were scanned withthe affymetrix genechip 7G plus, and AGCC is used for the image processing.
| | Sample_data_processing | RMA normalization using GeneSpring 7.3
| | Sample_platform_id | GPL570
| | Sample_contact_name | Biaoyang,,Lin
| | Sample_contact_email | bylin@u.washington.edu
| | Sample_contact_phone | 206 320 3570
| | Sample_contact_fax | 206 320 31 66
| | Sample_contact_department | Swedish Neuroscience Institute
| | Sample_contact_institute | Swedish Medical Center
| | Sample_contact_address | 550 17th Ave, James Tower suite 550
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98122
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519450/suppl/GSM519450.CEL.gz
| | Sample_series_id | GSE20689
| | Sample_data_row_count | 54675
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