Search results for the GEO ID: GSE20697 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM519587 | GPL570 |
|
hASC_t1_noFGF_rep1
|
hASC pre-adipocyte, day -2, no FGF
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519587
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519587/suppl/GSM519587.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519588 | GPL570 |
|
hASC_t1_noFGF_rep2
|
hASC pre-adipocyte, day -2, no FGF
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519588
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519588/suppl/GSM519588.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519589 | GPL570 |
|
hASC_t1_rep1
|
hASC pre-adipocyte, day -2
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519589
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519589/suppl/GSM519589.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519590 | GPL570 |
|
hASC_t1_rep2
|
hASC pre-adipocyte, day -2
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519590
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519590/suppl/GSM519590.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519591 | GPL570 |
|
hASC_t2_rep1
|
hASC pre-adipocyte, day 0
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519591
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519591/suppl/GSM519591.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519592 | GPL570 |
|
hASC_t2_rep2
|
hASC pre-adipocyte, day 0
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519592
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519592/suppl/GSM519592.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519593 | GPL570 |
|
hASC_t3_rep1
|
hASC pre-adipocyte, day 1
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519593
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519593/suppl/GSM519593.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519594 | GPL570 |
|
hASC_t4_rep1
|
hASC pre-adipocyte, day 2
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519594
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519594/suppl/GSM519594.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519595 | GPL570 |
|
hASC_t4_rep2
|
hASC pre-adipocyte, day 2
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519595
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519595/suppl/GSM519595.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519596 | GPL570 |
|
hASC_t5_rep1
|
hASC pre-adipocyte, day 3
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519596
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519596/suppl/GSM519596.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519597 | GPL570 |
|
hASC_t5_rep2
|
hASC pre-adipocyte, day 3
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519597
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519597/suppl/GSM519597.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519598 | GPL570 |
|
hASC_t6_rep1
|
hASC pre-adipocyte, day 5
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519598
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519598/suppl/GSM519598.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519599 | GPL570 |
|
hASC_t6_rep2
|
hASC pre-adipocyte, day 5
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519599
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519599/suppl/GSM519599.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519600 | GPL570 |
|
hASC_t7_rep1
|
hASC pre-adipocyte, day 7
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519600
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519600/suppl/GSM519600.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519601 | GPL570 |
|
hASC_t8_rep1
|
hASC pre-adipocyte, day 9
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519601
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519601/suppl/GSM519601.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519602 | GPL570 |
|
hASC_t8_rep2
|
hASC pre-adipocyte, day 9
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519602
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519602/suppl/GSM519602.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519603 | GPL570 |
|
hASC_t9_rep1
|
hASC pre-adipocyte, day 14
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519603
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519603/suppl/GSM519603.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
GSM519604 | GPL570 |
|
hASC_t9_rep2
|
hASC pre-adipocyte, day 14
|
gender: female
cell type: adipose stromal cells
|
no additional information
|
Sample_geo_accession | GSM519604
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Mar 09 2010
| Sample_last_update_date | Sep 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted using TRIzol (Invitrogen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by Affymetrix from 1 ug total RNA/sample
| Sample_hyb_protocol | As recommended by Affymetrix
| Sample_scan_protocol | As recommended by Affymetrix
| Sample_data_processing | The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
| Sample_platform_id | GPL570
| Sample_contact_name | Tarjei,S,Mikkelsen
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519604/suppl/GSM519604.CEL.gz
| Sample_series_id | GSE20697
| Sample_series_id | GSE20752
| Sample_data_row_count | 54675
| |
|
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