Search results for the GEO ID: GSE20715  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM519976 | GPL339 |
|
OuJ, air, biological rep 1
|
left lung lobe
|
treatment group: air
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 3-1
|
| Sample_geo_accession | GSM519976
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519976/suppl/GSM519976.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519977 | GPL339 |
|
OuJ, air, biological rep 2
|
left lung lobe
|
treatment group: air
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 3-2
|
| Sample_geo_accession | GSM519977
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519977/suppl/GSM519977.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519978 | GPL339 |
|
OuJ, air, biological rep 3
|
left lung lobe
|
treatment group: air
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 3-3
|
| Sample_geo_accession | GSM519978
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519978/suppl/GSM519978.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519979 | GPL339 |
|
OuJ, 6 hr Ozone, biological rep 1
|
left lung lobe
|
treatment group: 6 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 3-11
|
| Sample_geo_accession | GSM519979
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519979/suppl/GSM519979.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519980 | GPL339 |
|
OuJ, 6 hr Ozone, biological rep 2
|
left lung lobe
|
treatment group: 6 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 3-12
|
| Sample_geo_accession | GSM519980
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519980/suppl/GSM519980.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519981 | GPL339 |
|
OuJ, 6 hr Ozone, biological rep 3
|
left lung lobe
|
treatment group: 6 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 3-13
|
| Sample_geo_accession | GSM519981
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519981/suppl/GSM519981.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519982 | GPL339 |
|
OuJ, 24 hr Ozone, biological rep 1
|
left lung lobe
|
treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 10-100
|
| Sample_geo_accession | GSM519982
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519982/suppl/GSM519982.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519983 | GPL339 |
|
OuJ, 24 hr Ozone, biological rep 2
|
left lung lobe
|
treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 10-101
|
| Sample_geo_accession | GSM519983
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519983/suppl/GSM519983.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519984 | GPL339 |
|
OuJ, 24 hr Ozone, biological rep 3
|
left lung lobe
|
treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 10-102
|
| Sample_geo_accession | GSM519984
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519984/suppl/GSM519984.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519985 | GPL339 |
|
OuJ, 48 hr Ozone, biological rep 1
|
left lung lobe
|
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 10-110
|
| Sample_geo_accession | GSM519985
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519985/suppl/GSM519985.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519986 | GPL339 |
|
OuJ, 48 hr Ozone, biological rep 2
|
left lung lobe
|
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 10-111
|
| Sample_geo_accession | GSM519986
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519986/suppl/GSM519986.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519987 | GPL339 |
|
OuJ, 48 hr Ozone, biological rep 3
|
left lung lobe
|
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
|
3SRK 10-112
|
| Sample_geo_accession | GSM519987
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519987/suppl/GSM519987.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519988 | GPL339 |
|
HeJ, air, biological rep 1
|
left lung lobe
|
treatment group: air
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 3-21
|
| Sample_geo_accession | GSM519988
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519988/suppl/GSM519988.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519989 | GPL339 |
|
HeJ, air, biological rep 2
|
left lung lobe
|
treatment group: air
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 3-22 star
|
| Sample_geo_accession | GSM519989
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519989/suppl/GSM519989.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519990 | GPL339 |
|
HeJ, air, biological rep 3
|
left lung lobe
|
treatment group: air
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 3-23
|
| Sample_geo_accession | GSM519990
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519990/suppl/GSM519990.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519991 | GPL339 |
|
HeJ, 6 hr Ozone, biological rep 1
|
left lung lobe
|
treatment group: 6 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 3-31
|
| Sample_geo_accession | GSM519991
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519991/suppl/GSM519991.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519992 | GPL339 |
|
HeJ, 6 hr Ozone, biological rep 2
|
left lung lobe
|
treatment group: 6 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 3-33b
|
| Sample_geo_accession | GSM519992
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519992/suppl/GSM519992.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519993 | GPL339 |
|
HeJ, 6 hr Ozone, biological rep 3
|
left lung lobe
|
treatment group: 6 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 3-34
|
| Sample_geo_accession | GSM519993
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519993/suppl/GSM519993.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519994 | GPL339 |
|
HeJ, 24 hr Ozone, biological rep 1
|
left lung lobe
|
treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 13-1b
|
| Sample_geo_accession | GSM519994
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519994/suppl/GSM519994.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519995 | GPL339 |
|
HeJ, 24 hr Ozone, biological rep 2
|
left lung lobe
|
treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 13-2
|
| Sample_geo_accession | GSM519995
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519995/suppl/GSM519995.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519996 | GPL339 |
|
HeJ, 24 hr Ozone, biological rep 3
|
left lung lobe
|
treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 13-3b
|
| Sample_geo_accession | GSM519996
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519996/suppl/GSM519996.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519997 | GPL339 |
|
HeJ, 48 hr Ozone, biological rep 1
|
left lung lobe
|
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 13-11
|
| Sample_geo_accession | GSM519997
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519997/suppl/GSM519997.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519998 | GPL339 |
|
HeJ, 48 hr Ozone, biological rep 2
|
left lung lobe
|
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 13-12
|
| Sample_geo_accession | GSM519998
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519998/suppl/GSM519998.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
| | |
|
GSM519999 | GPL339 |
|
HeJ, 48 hr Ozone, biological rep 3
|
left lung lobe
|
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
3SRK 13-14
|
| Sample_geo_accession | GSM519999
| | Sample_status | Public on Feb 29 2012
| | Sample_submission_date | Mar 09 2010
| | Sample_last_update_date | Feb 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| | Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alison,Kendall,Bauer
| | Sample_contact_email | akbauer@msu.edu
| | Sample_contact_department | Pathobiology and Diagnostic Investigation
| | Sample_contact_institute | Michigan State University
| | Sample_contact_address | B43 Food Safety and Toxicology Bldg.
| | Sample_contact_city | East Lansing
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48824
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM519nnn/GSM519999/suppl/GSM519999.CEL.gz
| | Sample_series_id | GSE20715
| | Sample_data_row_count | 22690
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