Search results for the GEO ID: GSE20854  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM521595 | GPL570 |
|
Hec50co cells, undosed, harvested at 12 hr, rep1
|
type II endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521595
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521595/suppl/GSM521595.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521595/suppl/GSM521595.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521596 | GPL570 |
|
Hec50co cells, undosed, harvested at 12 hr, rep2
|
type II endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521596
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521596/suppl/GSM521596.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521596/suppl/GSM521596.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521597 | GPL570 |
|
Hec50co cells, dosed with egf, harvested at 12 hr, rep1
|
type II endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
| Sample_geo_accession | GSM521597
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521597/suppl/GSM521597.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521597/suppl/GSM521597.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521598 | GPL570 |
|
Hec50co cells, dosed with egf, harvested at 12 hr, rep2
|
type II endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
| Sample_geo_accession | GSM521598
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521598/suppl/GSM521598.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521598/suppl/GSM521598.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521599 | GPL570 |
|
Hec50co cells, dosed with iressa, harvested at 12 hr, rep1
|
type II endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
| Sample_geo_accession | GSM521599
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521599/suppl/GSM521599.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521599/suppl/GSM521599.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521600 | GPL570 |
|
Hec50co cells, dosed with iressa, harvested at 12 hr, rep2
|
type II endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
| Sample_geo_accession | GSM521600
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521600/suppl/GSM521600.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521600/suppl/GSM521600.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521601 | GPL570 |
|
Hec50co cells, undosed, harvested at 24 hr, rep1
|
type II endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521601
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521601/suppl/GSM521601.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521601/suppl/GSM521601.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521602 | GPL570 |
|
Hec50co cells, undosed, harvested at 24 hr, rep2
|
type II endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521602
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521602/suppl/GSM521602.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521602/suppl/GSM521602.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521603 | GPL570 |
|
Hec50co cells, dosed with egf, harvested at 24 hr, rep1
|
type II endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
| Sample_geo_accession | GSM521603
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521603/suppl/GSM521603.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521603/suppl/GSM521603.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521604 | GPL570 |
|
Hec50co cells, dosed with egf, harvested at 24 hr, rep2
|
type II endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
| Sample_geo_accession | GSM521604
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521604/suppl/GSM521604.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521604/suppl/GSM521604.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521605 | GPL570 |
|
Hec50co cells, dosed with iressa, harvested at 24 hr, rep1
|
type II endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
| Sample_geo_accession | GSM521605
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521605/suppl/GSM521605.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521605/suppl/GSM521605.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521606 | GPL570 |
|
Hec50co cells, dosed with iressa, harvested at 24 hr, rep2
|
type II endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
tissue type: Type II endometrial adenocarcinoma
cell line: Hec50co
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
| Sample_geo_accession | GSM521606
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521606/suppl/GSM521606.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521606/suppl/GSM521606.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521607 | GPL570 |
|
Ishikawa Hcells, undosed, harvested at 12 hr, rep1
|
type I endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521607
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521607/suppl/GSM521607.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521607/suppl/GSM521607.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521608 | GPL570 |
|
Ishikawa Hcells, undosed, harvested at 12 hr, rep2
|
type I endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521608
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521608/suppl/GSM521608.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521608/suppl/GSM521608.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521609 | GPL570 |
|
Ishikawa Hcells, dosed with egf, harvested at 12 hr, rep1
|
type I endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
| Sample_geo_accession | GSM521609
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521609/suppl/GSM521609.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521609/suppl/GSM521609.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521610 | GPL570 |
|
Ishikawa Hcells, dosed with egf, harvested at 12 hr, rep2
|
type I endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with EGF
|
| Sample_geo_accession | GSM521610
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521610/suppl/GSM521610.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521610/suppl/GSM521610.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521611 | GPL570 |
|
Ishikawa Hcells, dosed with iressa, harvested at 12 hr, rep1
|
type I endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
| Sample_geo_accession | GSM521611
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521611/suppl/GSM521611.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521611/suppl/GSM521611.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521612 | GPL570 |
|
Ishikawa Hcells, dosed with iressa, harvested at 12 hr, rep2
|
type I endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 12 hr dosed with Iressa
|
| Sample_geo_accession | GSM521612
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521612/suppl/GSM521612.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521612/suppl/GSM521612.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521613 | GPL570 |
|
Ishikawa Hcells, undosed, harvested at 24 hr, rep1
|
type I endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521613
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521613/suppl/GSM521613.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521613/suppl/GSM521613.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521614 | GPL570 |
|
Ishikawa Hcells, undosed, harvested at 24 hr, rep2
|
type I endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr undosed (DMSO)
|
| Sample_geo_accession | GSM521614
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521614/suppl/GSM521614.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521614/suppl/GSM521614.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521615 | GPL570 |
|
Ishikawa Hcells, dosed with egf, harvested at 24 hr, rep1
|
type I endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
| Sample_geo_accession | GSM521615
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521615/suppl/GSM521615.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521615/suppl/GSM521615.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521616 | GPL570 |
|
Ishikawa Hcells, dosed with egf, harvested at 24 hr, rep2
|
type I endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with EGF
|
| Sample_geo_accession | GSM521616
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521616/suppl/GSM521616.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521616/suppl/GSM521616.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521617 | GPL570 |
|
Ishikawa Hcells, dosed with iressa, harvested at 24 hr, rep1
|
type I endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
| Sample_geo_accession | GSM521617
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521617/suppl/GSM521617.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521617/suppl/GSM521617.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
GSM521618 | GPL570 |
|
Ishikawa Hcells, dosed with iressa, harvested at 24 hr, rep2
|
type I endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
tissue type: Type I endometrial adenocarcinoma
cell line: Ishikawa H
|
Gene expression data from human endometrial adenocarcinoma cell line 24 hr dosed with Iressa
|
| Sample_geo_accession | GSM521618
| | Sample_status | Public on Mar 13 2010
| | Sample_submission_date | Mar 12 2010
| | Sample_last_update_date | Mar 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The two different tissue culture cell lines were dosed with 0.1% DMSO (vehicle=undosed) or with 1uM gefitinib, or with 30ng/ml EGF for 12 or 24 hours then the cells were harvested and total RNA extracted and purified.
| | Sample_growth_protocol_ch1 | Hec50co cells and Ishikawa Hcells were cultured in DMEM containing 10% FBS substitute and 2mM L-Glutamine and 1x antibiotic-antimycotic solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gavin,,Pickett
| | Sample_contact_laboratory | KUGR
| | Sample_contact_department | Genomics
| | Sample_contact_institute | UNM
| | Sample_contact_address | 2325 Camino de Salud NE
| | Sample_contact_city | Albuquerque
| | Sample_contact_state | NM
| | Sample_contact_zip/postal_code | 87131
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521618/suppl/GSM521618.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM521nnn/GSM521618/suppl/GSM521618.CHP.gz
| | Sample_series_id | GSE20854
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
