Result

Search results for the GEO ID: GSE20899

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Source name

Description

Characteristics

GSM522421

GPL1261

undifferentiated ES cells

cell type: undifferentiated ES cells

1531 Gene expression data from the undifferentiated ES cells

Sample_geo_accession	GSM522421
Sample_status	Public on Mar 16 2010
Sample_submission_date	Mar 16 2010
Sample_last_update_date	Mar 16 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The HB9-GFP+ cells were sorted and collected on day 8.
Sample_growth_protocol_ch1	HB9-ES cells were maintained on mitomycin C-treated mouse embryonic fibroblast cells in high-glucose DMEM supplemented with 15% fetal bovine serum, 2 mM glutamine, 0.1 mM nonessential amino acids, 1 mM pyruvate, 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), and 1000 U/ml leukemia inhibitory factor (Chemicon). The day on which ES cells were seeded to differentiate is defined as differentiation day 0. Exogenous sonic hedgehog (Shh; 200 uM, R&D Systems), retinoic acid (0.1 uM, Sigma-Aldrich) and purmorphamine (0.2 uM, Tocris) were added into the differentiation medium from day 5 to day 9 and were replaced every other day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. The quality of RNA was carefully evaluated by the electrophoresis and Agilent Bioanalyzer 2100.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20-50 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array, Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G
Sample_data_processing	The data were analyzed with GeneSpring GX software version 7.3 using GCRMA as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Chi-Chung,,Wang
Sample_contact_institute	Fu Jen Catholic University
Sample_contact_address	No. 510, Jhongjheng Rd.,
Sample_contact_city	Sinjhuang
Sample_contact_zip/postal_code	242
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM522nnn/GSM522421/suppl/GSM522421.CEL.gz
Sample_series_id	GSE20899
Sample_data_row_count	45101

GSM522422

GPL1261

mouse cerebellar granule cells on postnatal day 6

cell type: cerebellar granule cells

1532 Gene expression data from the mouse cerebellar granule cells on postnatal day 6

Sample_geo_accession	GSM522422
Sample_status	Public on Mar 16 2010
Sample_submission_date	Mar 16 2010
Sample_last_update_date	Mar 16 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The HB9-GFP+ cells were sorted and collected on day 8.
Sample_growth_protocol_ch1	HB9-ES cells were maintained on mitomycin C-treated mouse embryonic fibroblast cells in high-glucose DMEM supplemented with 15% fetal bovine serum, 2 mM glutamine, 0.1 mM nonessential amino acids, 1 mM pyruvate, 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), and 1000 U/ml leukemia inhibitory factor (Chemicon). The day on which ES cells were seeded to differentiate is defined as differentiation day 0. Exogenous sonic hedgehog (Shh; 200 uM, R&D Systems), retinoic acid (0.1 uM, Sigma-Aldrich) and purmorphamine (0.2 uM, Tocris) were added into the differentiation medium from day 5 to day 9 and were replaced every other day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. The quality of RNA was carefully evaluated by the electrophoresis and Agilent Bioanalyzer 2100.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20-50 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array, Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G
Sample_data_processing	The data were analyzed with GeneSpring GX software version 7.3 using GCRMA as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Chi-Chung,,Wang
Sample_contact_institute	Fu Jen Catholic University
Sample_contact_address	No. 510, Jhongjheng Rd.,
Sample_contact_city	Sinjhuang
Sample_contact_zip/postal_code	242
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM522nnn/GSM522422/suppl/GSM522422.CEL.gz
Sample_series_id	GSE20899
Sample_data_row_count	45101

GSM522423

GPL1261

sorted HB9-GFP+ cells

cell type: HB9 transgenic ES-GFP+ cells

1557 Gene expression data from the sorted HB9-GFP+ cells

Sample_geo_accession	GSM522423
Sample_status	Public on Mar 16 2010
Sample_submission_date	Mar 16 2010
Sample_last_update_date	Mar 16 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The HB9-GFP+ cells were sorted and collected on day 8.
Sample_growth_protocol_ch1	HB9-ES cells were maintained on mitomycin C-treated mouse embryonic fibroblast cells in high-glucose DMEM supplemented with 15% fetal bovine serum, 2 mM glutamine, 0.1 mM nonessential amino acids, 1 mM pyruvate, 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), and 1000 U/ml leukemia inhibitory factor (Chemicon). The day on which ES cells were seeded to differentiate is defined as differentiation day 0. Exogenous sonic hedgehog (Shh; 200 uM, R&D Systems), retinoic acid (0.1 uM, Sigma-Aldrich) and purmorphamine (0.2 uM, Tocris) were added into the differentiation medium from day 5 to day 9 and were replaced every other day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. The quality of RNA was carefully evaluated by the electrophoresis and Agilent Bioanalyzer 2100.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20-50 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array, Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G
Sample_data_processing	The data were analyzed with GeneSpring GX software version 7.3 using GCRMA as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Chi-Chung,,Wang
Sample_contact_institute	Fu Jen Catholic University
Sample_contact_address	No. 510, Jhongjheng Rd.,
Sample_contact_city	Sinjhuang
Sample_contact_zip/postal_code	242
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM522nnn/GSM522423/suppl/GSM522423.CEL.gz
Sample_series_id	GSE20899
Sample_data_row_count	45101

GSM522424

GPL1261

sorted sox1 neural stem cells

cell type: neural stem cells

1602 Gene expression data from the sorted sox1 neural stem cells

Sample_geo_accession	GSM522424
Sample_status	Public on Mar 16 2010
Sample_submission_date	Mar 16 2010
Sample_last_update_date	Mar 16 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The HB9-GFP+ cells were sorted and collected on day 8.
Sample_growth_protocol_ch1	HB9-ES cells were maintained on mitomycin C-treated mouse embryonic fibroblast cells in high-glucose DMEM supplemented with 15% fetal bovine serum, 2 mM glutamine, 0.1 mM nonessential amino acids, 1 mM pyruvate, 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), and 1000 U/ml leukemia inhibitory factor (Chemicon). The day on which ES cells were seeded to differentiate is defined as differentiation day 0. Exogenous sonic hedgehog (Shh; 200 uM, R&D Systems), retinoic acid (0.1 uM, Sigma-Aldrich) and purmorphamine (0.2 uM, Tocris) were added into the differentiation medium from day 5 to day 9 and were replaced every other day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. The quality of RNA was carefully evaluated by the electrophoresis and Agilent Bioanalyzer 2100.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20-50 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array, Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G
Sample_data_processing	The data were analyzed with GeneSpring GX software version 7.3 using GCRMA as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Chi-Chung,,Wang
Sample_contact_institute	Fu Jen Catholic University
Sample_contact_address	No. 510, Jhongjheng Rd.,
Sample_contact_city	Sinjhuang
Sample_contact_zip/postal_code	242
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM522nnn/GSM522424/suppl/GSM522424.CEL.gz
Sample_series_id	GSE20899
Sample_data_row_count	45101

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