Search results for the GEO ID: GSE20935  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM523581 | GPL1355 |
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Splenic rat NKR-P1B+ NK cells
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Splenic NKR-P1B+ NK cells
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strain: PVG.7b
age: 8-12 weeks
tissue: spleen
cell type: NKR-P1B+ NK cells
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Gene expression in NKR-P1B+ NK cells.
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| Sample_geo_accession | GSM523581
| | Sample_status | Public on Mar 18 2010
| | Sample_submission_date | Mar 17 2010
| | Sample_last_update_date | Mar 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Mononuclear spleen cells prepared by Lymphoprep separation, and enriched for NK cells by precoating cells with anti-TCRαβ, anti-CD68, anti-CD5, anti-Igκ-chain, and anti-CD45RA supernatants followed by depletions with anti-mouse IgG-coated Dynabeads (Invitrogen Dynal). The enriched population was stained with antibodies towards rat CD3, NKR-P1A, Ly49s3, and NKR-P1B. Single-positive NKR-P1B+ or Ly49s3+ NK cells (CD3-NKR-P1A+) were sorted in a FACS-Diva (BD Biosciences), and the purity of sorted cells was above 90%. Cells were lysed in Tri Reagent (Sigma-Aldrich) for total RNA harvest.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tri Reagent extraction of total RNA was performed according to the manufacturer's instructions (Sigma-Aldrich).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Three µg of total RNA were used to generate cRNA, subsequently hybridized to the Affymetrix GeneChip® Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | The array was scanned using GeneArray scanner (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Marit,,Inngjerdingen
| | Sample_contact_email | marit.inngjerdingen@rr-research.no
| | Sample_contact_phone | +4723073769
| | Sample_contact_fax | +47230735 10
| | Sample_contact_laboratory | NK cell research group (prof. Vaage)
| | Sample_contact_department | Institute of Immunology
| | Sample_contact_institute | Oslo University Hospital, Rikshospitalet and University of Oslo
| | Sample_contact_address | Sognsvannsveien 20
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | 0027
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523581/suppl/GSM523581.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523581/suppl/GSM523581.CHP.gz
| | Sample_series_id | GSE20935
| | Sample_data_row_count | 31099
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GSM523582 | GPL1355 |
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Splenic rat Ly49s3+ NK cells
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Splenic Ly49s3+ NK cells
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strain: PVG.7b
age: 8-12 weeks
tissue: spleen
cell type: Ly49s3+ NK cells
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Gene expression in Ly49s3+ NK cells.
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| Sample_geo_accession | GSM523582
| | Sample_status | Public on Mar 18 2010
| | Sample_submission_date | Mar 17 2010
| | Sample_last_update_date | Mar 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Mononuclear spleen cells prepared by Lymphoprep separation, and enriched for NK cells by precoating cells with anti-TCRαβ, anti-CD68, anti-CD5, anti-Igκ-chain, and anti-CD45RA supernatants followed by depletions with anti-mouse IgG-coated Dynabeads (Invitrogen Dynal). The enriched population was stained with antibodies towards rat CD3, NKR-P1A, Ly49s3, and NKR-P1B. Single-positive NKR-P1B+ or Ly49s3+ NK cells (CD3-NKR-P1A+) were sorted in a FACS-Diva (BD Biosciences), and the purity of sorted cells was above 90%. Cells were lysed in Tri Reagent (Sigma-Aldrich) for total RNA harvest.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tri Reagent extraction of total RNA was performed according to the manufacturer's instructions (Sigma-Aldrich).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Three µg of total RNA were used to generate cRNA, subsequently hybridized to the Affymetrix GeneChip® Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | The array was scanned using GeneArray scanner (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Marit,,Inngjerdingen
| | Sample_contact_email | marit.inngjerdingen@rr-research.no
| | Sample_contact_phone | +4723073769
| | Sample_contact_fax | +47230735 10
| | Sample_contact_laboratory | NK cell research group (prof. Vaage)
| | Sample_contact_department | Institute of Immunology
| | Sample_contact_institute | Oslo University Hospital, Rikshospitalet and University of Oslo
| | Sample_contact_address | Sognsvannsveien 20
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | 0027
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523582/suppl/GSM523582.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523582/suppl/GSM523582.CHP.gz
| | Sample_series_id | GSE20935
| | Sample_data_row_count | 31099
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