Search results for the GEO ID: GSE20950  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM523837 | GPL570 |
|
Insulin resistant patient 7 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523837
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523837/suppl/GSM523837.CEL.gz
| | Sample_relation | Reanalysis of: GSM395775
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523838 | GPL570 |
|
Insulin resistant patient 8 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523838
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523838/suppl/GSM523838.CEL.gz
| | Sample_relation | Reanalysis of: GSM395776
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523839 | GPL570 |
|
Insulin resistant patient 9 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523839
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523839/suppl/GSM523839.CEL.gz
| | Sample_relation | Reanalysis of: GSM395777
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523840 | GPL570 |
|
Insulin resistant patient 10 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523840
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523840/suppl/GSM523840.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523841 | GPL570 |
|
Insulin resistant patient 11 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523841
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523841/suppl/GSM523841.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523842 | GPL570 |
|
Insulin resistant patient 12 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523842
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523842/suppl/GSM523842.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523843 | GPL570 |
|
Insulin resistant patient 13 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523843
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523843/suppl/GSM523843.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523844 | GPL570 |
|
Insulin resistant patient 14 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523844
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523844/suppl/GSM523844.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523845 | GPL570 |
|
Insulin resistant patient 15 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523845
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523845/suppl/GSM523845.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523846 | GPL570 |
|
Insulin sensitive patient 6 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523846
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523846/suppl/GSM523846.CEL.gz
| | Sample_relation | Reanalysis of: GSM395778
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523847 | GPL570 |
|
Insulin sensitive patient 7 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523847
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523847/suppl/GSM523847.CEL.gz
| | Sample_relation | Reanalysis of: GSM395779
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523848 | GPL570 |
|
Insulin sensitive patient 8 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523848
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523848/suppl/GSM523848.CEL.gz
| | Sample_relation | Reanalysis of: GSM395780
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523849 | GPL570 |
|
Insulin sensitive patient 9 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523849
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523849/suppl/GSM523849.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523850 | GPL570 |
|
Insulin sensitive patient 10 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523850
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523850/suppl/GSM523850.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523851 | GPL570 |
|
Insulin sensitive patient 11 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523851
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523851/suppl/GSM523851.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523852 | GPL570 |
|
Insulin sensitive patient 12 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523852
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523852/suppl/GSM523852.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523853 | GPL570 |
|
Insulin sensitive patient 13 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523853
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523853/suppl/GSM523853.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523854 | GPL570 |
|
Insulin sensitive patient 14 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523854
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523854/suppl/GSM523854.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523855 | GPL570 |
|
Insulin sensitive patient 15 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523855
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523855/suppl/GSM523855.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523856 | GPL570 |
|
Insulin resistant patient 6 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523856
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523856/suppl/GSM523856.CEL.gz
| | Sample_relation | Reanalysis of: GSM395784
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523857 | GPL570 |
|
Insulin resistant patient 7 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523857
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523857/suppl/GSM523857.CEL.gz
| | Sample_relation | Reanalysis of: GSM395785
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523858 | GPL570 |
|
Insulin resistant patient 8 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523858
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523858/suppl/GSM523858.CEL.gz
| | Sample_relation | Reanalysis of: GSM395786
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523859 | GPL570 |
|
Insulin resistant patient 9 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523859
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523859/suppl/GSM523859.CEL.gz
| | Sample_relation | Reanalysis of: GSM395787
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523860 | GPL570 |
|
Insulin resistant patient 10 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523860
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523860/suppl/GSM523860.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523861 | GPL570 |
|
Insulin resistant patient 11 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523861
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523861/suppl/GSM523861.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523862 | GPL570 |
|
Insulin resistant patient 12 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523862
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523862/suppl/GSM523862.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523863 | GPL570 |
|
Insulin resistant patient 13 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523863
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523863/suppl/GSM523863.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523864 | GPL570 |
|
Insulin resistant patient 14 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523864
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523864/suppl/GSM523864.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523865 | GPL570 |
|
Insulin resistant patient 15 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523865
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523865/suppl/GSM523865.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523866 | GPL570 |
|
Insulin sensitive patient 6 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523866
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523866/suppl/GSM523866.CEL.gz
| | Sample_relation | Reanalysis of: GSM395788
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523867 | GPL570 |
|
Insulin sensitive patient 7 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523867
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523867/suppl/GSM523867.CEL.gz
| | Sample_relation | Reanalysis of: GSM395789
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523868 | GPL570 |
|
Insulin sensitive patient 8 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523868
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523868/suppl/GSM523868.CEL.gz
| | Sample_relation | Reanalysis of: GSM395790
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523869 | GPL570 |
|
Insulin sensitive patient 9 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523869
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523869/suppl/GSM523869.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523870 | GPL570 |
|
Insulin sensitive patient 10 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523870
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523870/suppl/GSM523870.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523871 | GPL570 |
|
Insulin sensitive patient 11 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523871
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523871/suppl/GSM523871.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523872 | GPL570 |
|
Insulin sensitive patient 12 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523872
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523872/suppl/GSM523872.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523873 | GPL570 |
|
Insulin sensitive patient 13 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523873
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523873/suppl/GSM523873.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523874 | GPL570 |
|
Insulin sensitive patient 14 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523874
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523874/suppl/GSM523874.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
GSM523875 | GPL570 |
|
Insulin sensitive patient 15 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM523875
| | Sample_status | Public on Mar 25 2010
| | Sample_submission_date | Mar 18 2010
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523875/suppl/GSM523875.CEL.gz
| | Sample_series_id | GSE20950
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
