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Search results for the GEO ID: GSE20958

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Title

Source name

Description

Characteristics

GSM523995

GPL1261

Bmi1 embryoid body sample_1 (Bmi1_EB_1)

Embryoid body formation (10 days) of Bmi1 transduced embryonic stem cells (CCE ES cells).

strain: 129/Sv

BioConductor software package (Gentleman, R. C., Carey, V. J., Bates, D. M. et al. Bioconductor: Open software development for computational biology and bioinformatics. Genome Biol. 2004; 5: R80) (www.bioconductor.org).

Sample_geo_accession	GSM523995
Sample_status	Public on Apr 29 2011
Sample_submission_date	Mar 19 2010
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CCE ES cells were trypsinized and subjected to embryoid body (EB) assay in bacterial Petri dish. At day 10 EBs were harvested and total RNA was isolated.
Sample_growth_protocol_ch1	CCE ES cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above. For differentiation CCE ES cells were subjected to EB assay (10 days).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523995/suppl/GSM523995.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM523996

GPL1261

Bmi1 embryoid body sample_2 (Bmi1_EB_2)

Embryoid body formation (10 days) of Bmi1 transduced embryonic stem cells (CCE ES cells).

strain: 129/Sv

Sample_geo_accession	GSM523996
Sample_status	Public on Apr 29 2011
Sample_submission_date	Mar 19 2010
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CCE ES cells were trypsinized and subjected to embryoid body (EB) assay in bacterial Petri dish. At day 10 EBs were harvested and total RNA was isolated.
Sample_growth_protocol_ch1	CCE ES cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above. For differentiation CCE ES cells were subjected to EB assay (10 days).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523996/suppl/GSM523996.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM523997

GPL1261

Empty vector control embryoid body sample_1 (Vector_EB_1)

Embryoid body formation (10 days) of empty vector transduced embryonic stem cells (CCE ES cells).

strain: 129/Sv

Sample_geo_accession	GSM523997
Sample_status	Public on Apr 29 2011
Sample_submission_date	Mar 19 2010
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CCE ES cells were trypsinized and subjected to embryoid body (EB) assay in bacterial Petri dish. At day 10 EBs were harvested and total RNA was isolated.
Sample_growth_protocol_ch1	CCE ES cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above. For differentiation CCE ES cells were subjected to EB assay (10 days).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523997/suppl/GSM523997.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM523998

GPL1261

Empty vector control embryoid body sample_2 (Vector_EB_2)

Embryoid body formation (10 days) of empty vector transduced embryonic stem cells (CCE ES cells).

strain: 129/Sv

Sample_geo_accession	GSM523998
Sample_status	Public on Apr 29 2011
Sample_submission_date	Mar 19 2010
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CCE ES cells were trypsinized and subjected to embryoid body (EB) assay in bacterial Petri dish. At day 10 EBs were harvested and total RNA was isolated.
Sample_growth_protocol_ch1	CCE ES cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above. For differentiation CCE ES cells were subjected to EB assay (10 days).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM523nnn/GSM523998/suppl/GSM523998.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM706697

GPL1261

Bmi1 embryonic stem cells sample_1 (Bmi1_ESC_1)

Bmi1 transduced embryonic stem cells (Bmi1 ES cells).

strain: 129/Sv

Sample_geo_accession	GSM706697
Sample_status	Public on Apr 29 2011
Sample_submission_date	Apr 12 2011
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Bmi1 transduced embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF).
Sample_growth_protocol_ch1	Bmi1 transduced embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706697/suppl/GSM706697.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM706698

GPL1261

Bmi1 embryonic stem cells sample_2 (Bmi1_ESC_2)

Bmi1 transduced embryonic stem cells (Bmi1 ES cells).

strain: 129/Sv

Sample_geo_accession	GSM706698
Sample_status	Public on Apr 29 2011
Sample_submission_date	Apr 12 2011
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Bmi1 transduced embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF).
Sample_growth_protocol_ch1	Bmi1 transduced embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706698/suppl/GSM706698.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM706700

GPL1261

Untreated CCE embryonic stem cells (CCE_ESC_Control)

CCE embryonic stem cells (CCE ES cells).

strain: 129/Sv

Sample_geo_accession	GSM706700
Sample_status	Public on Apr 29 2011
Sample_submission_date	Apr 12 2011
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CCE embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF).
Sample_growth_protocol_ch1	CCE embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706700/suppl/GSM706700.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

GSM706701

GPL1261

Empty vector CCE embryonic stem cells (CCE_ESC_Vector)

Empty vector transduced CCE embryonic stem cells (empty vector CCE ES cells)

strain: 129/Sv

Sample_geo_accession	GSM706701
Sample_status	Public on Apr 29 2011
Sample_submission_date	Apr 12 2011
Sample_last_update_date	Apr 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Empty vector transduced CCE embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF).
Sample_growth_protocol_ch1	Empty vector transduced CCE embryonic stem cells were cultured on gelatin coated dish in DMEM medium with 15% FSC and leukemia inhibitory factor (LIF). Lentivirus containing mouse BMI1 cDNA was used to infect CCE ES cells in the presence of 8 microgram polybrene. Empty vector served as control. After two passages eGFP positive CCE ES cells were sorted by flow cytometry and cells were further grown in culture medium as above.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany) with DNAse digestion using the maufacturer's buffers and protocols. RNA quality was assessed by Agilent 2100 Bioanalyzer System.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe preparation was with 1 microgram RNA using the Affymetrix One-Cycle Target Labeling Kit according to the manufacturer's instructions. Labeled RNA was fragmented by heating to 94°C for 35 minutes in Fragmentatin buffer of the compnay.
Sample_hyb_protocol	Affymetrix arrays were prehybridized with 200 microliter MES hybridization buffer for 10 minutes at 45˚C with rotation (60 U/min) in an Affymetrix Hybridization Oven 640. After removing the prehybridization solution, 10 microgram of labeled cRNA in 200 microliter MES hybridization buffer were applied to the array leaving a small air bubble. Arrays were incubated overnight at 45˚C with rotation (60U/min) in the hybridization chamber.
Sample_scan_protocol	Arrays were scanned using Affymetrix GeneChip Scanner 3000.
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Martin,,Zenke
Sample_contact_email	Martin.Zenke@rwth-aachen.de
Sample_contact_phone	+49-241-80 80760
Sample_contact_department	Cell Biology
Sample_contact_institute	Institute for Biomedical Engineering
Sample_contact_address	Universitatsklinikum Aachen, RWTH
Sample_contact_city	Aachen
Sample_contact_state	NRW
Sample_contact_zip/postal_code	52074
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706701/suppl/GSM706701.CEL.gz
Sample_series_id	GSE20958
Sample_data_row_count	45101

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