Search results for the GEO ID: GSE21031  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM525382 | GPL1261 |
|
IL6_0h1_MOE430_2
|
hepatocytes, 0h
|
tissue: primary mouse hepatocytes
treatment: unstimulated
time: 0 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_0h1_MOE430_2
|
| Sample_geo_accession | GSM525382
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525382/suppl/GSM525382.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525383 | GPL1261 |
|
IL6_0h2_MOE430_2
|
hepatocytes, 0h
|
tissue: primary mouse hepatocytes
treatment: unstimulated
time: 0 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_0h2_MOE430_2
|
| Sample_geo_accession | GSM525383
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525383/suppl/GSM525383.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525384 | GPL1261 |
|
IL6_0h3_MOE430_2
|
hepatocytes, 0h
|
tissue: primary mouse hepatocytes
treatment: unstimulated
time: 0 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_0h3_MOE430_2
|
| Sample_geo_accession | GSM525384
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525384/suppl/GSM525384.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525385 | GPL1261 |
|
IL6_1h1_MOE430_2
|
hepatocytes, Il-6, 1h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 1 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_1h1_MOE430_2
|
| Sample_geo_accession | GSM525385
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525385/suppl/GSM525385.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525386 | GPL1261 |
|
IL6_1h2_MOE430_2
|
hepatocytes, Il-6, 1h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 1 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_1h2_MOE430_2
|
| Sample_geo_accession | GSM525386
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525386/suppl/GSM525386.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525387 | GPL1261 |
|
IL6_1h3_MOE430_2
|
hepatocytes, Il-6, 1h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 1 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_1h3_MOE430_2
|
| Sample_geo_accession | GSM525387
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525387/suppl/GSM525387.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525388 | GPL1261 |
|
IL6_2h1_MOE430_2
|
hepatocytes, Il-6, 2h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 2 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_2h1_MOE430_2
|
| Sample_geo_accession | GSM525388
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525388/suppl/GSM525388.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525389 | GPL1261 |
|
IL6_2h2_MOE430_2
|
hepatocytes, Il-6, 2h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 2 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_2h2_MOE430_2
|
| Sample_geo_accession | GSM525389
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525389/suppl/GSM525389.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525390 | GPL1261 |
|
IL6_2h3_MOE430_2
|
hepatocytes, Il-6, 2h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 2 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_2h3_MOE430_2
|
| Sample_geo_accession | GSM525390
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525390/suppl/GSM525390.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525391 | GPL1261 |
|
IL6_4h1_MOE430_2
|
hepatocytes, Il-6, 4h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 4 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_4h1_MOE430_2
|
| Sample_geo_accession | GSM525391
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525391/suppl/GSM525391.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525392 | GPL1261 |
|
IL6_4h2_MOE430_2
|
hepatocytes, Il-6, 4h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 4 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_4h2_MOE430_2
|
| Sample_geo_accession | GSM525392
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525392/suppl/GSM525392.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
GSM525393 | GPL1261 |
|
IL6_4h3_MOE430_2
|
hepatocytes, Il-6, 4h
|
tissue: primary mouse hepatocytes
treatment: IL-6
time: 4 h
|
GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix).
Frahm_250406_2303_IL6_4h3_MOE430_2
|
| Sample_geo_accession | GSM525393
| | Sample_status | Public on Mar 24 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Mar 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Primary hepatocytes were serum starved as described before (Klingmüller et al., 2006). After 5 hours of starvation, cells were stimulated with 1nM IL-6 for 0, 1, 2, or 4 hours.
| | Sample_growth_protocol_ch1 | Primary hepatocytes were isolated and cultivated as described previously (Klingmüller et al., 2006).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The quality of total RNA samples extracted from 2x 10^6 primary mouse hepatocytes was assessed with the Bioanalyzer 2100 (Agilent) to ensure that 28S/18S rRNA ratios were in the range of 1.5 to 2.0 and concentrations were comparable between samples. For each time point, 4 μg of total RNA were used for the hybridization procedure using the One-Cycle Target Labeling Kit (Affymetrix).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the One-Cycle Target Labeling Assay from 2.5ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16h at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Fluorescence intensities of the hybridized arrays were acquired with the GeneChip Scanner 3000 and the operating software GCOS (Affymetrix).
| | Sample_data_processing | Data processing was performed using the Limma toolbox (Smyth et al., 2005) provided by Bioconductor (Gentleman et al., 2004). The RMA approach was used for normalization and background correction. In addition, probe sets were filtered out by the genefilter package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Roller
| | Sample_contact_institute | Munich Leukemia Laboratory
| | Sample_contact_address | Max-Lebsche-Platz 31
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525393/suppl/GSM525393.CEL.gz
| | Sample_series_id | GSE21031
| | Sample_data_row_count | 45101
| | |
|
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