Search results for the GEO ID: GSE21033  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM525394 | GPL1261 |
|
MouseDC-0hr-1
|
bone marrow DC, poly I:C, 0hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 0hr, rep1
|
| Sample_geo_accession | GSM525394
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525394/suppl/GSM525394.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525394/suppl/GSM525394.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525395 | GPL1261 |
|
MouseDC-0hr-2
|
bone marrow DC, poly I:C, 0hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 0hr, rep2
|
| Sample_geo_accession | GSM525395
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525395/suppl/GSM525395.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525395/suppl/GSM525395.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525396 | GPL1261 |
|
MouseDC-1hr-1
|
bone marrow DC, poly I:C, 1hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 1hr, rep1
|
| Sample_geo_accession | GSM525396
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525396/suppl/GSM525396.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525396/suppl/GSM525396.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525397 | GPL1261 |
|
MouseDC-1hr-2
|
bone marrow DC, poly I:C, 1hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 1hr, rep2
|
| Sample_geo_accession | GSM525397
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525397/suppl/GSM525397.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525397/suppl/GSM525397.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525398 | GPL1261 |
|
MouseDC-3hr-1
|
bone marrow DC, poly I:C, 3hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 3hr, rep1
|
| Sample_geo_accession | GSM525398
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525398/suppl/GSM525398.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525398/suppl/GSM525398.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525399 | GPL1261 |
|
MouseDC-3hr-2
|
bone marrow DC, poly I:C, 3hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 3hr, rep2
|
| Sample_geo_accession | GSM525399
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525399/suppl/GSM525399.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525399/suppl/GSM525399.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525400 | GPL1261 |
|
MouseDC-6hr-1
|
bone marrow DC, poly I:C, 6hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 6hr, rep1
|
| Sample_geo_accession | GSM525400
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525400/suppl/GSM525400.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525400/suppl/GSM525400.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525401 | GPL1261 |
|
MouseDC-6hr-2
|
bone marrow DC, poly I:C, 6hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 6hr, rep2
|
| Sample_geo_accession | GSM525401
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525401/suppl/GSM525401.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525401/suppl/GSM525401.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525402 | GPL1261 |
|
MouseDC-12hr-1
|
bone marrow DC, poly I:C, 12hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 12hr, rep1
|
| Sample_geo_accession | GSM525402
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525402/suppl/GSM525402.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525402/suppl/GSM525402.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525403 | GPL1261 |
|
MouseDC-12hr-2
|
bone marrow DC, poly I:C, 12hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 12hr, rep2
|
| Sample_geo_accession | GSM525403
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525403/suppl/GSM525403.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525403/suppl/GSM525403.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525404 | GPL1261 |
|
MouseDC-24hr-1
|
bone marrow DC, poly I:C, 24hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 24hr, rep1
|
| Sample_geo_accession | GSM525404
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525404/suppl/GSM525404.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525404/suppl/GSM525404.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
GSM525405 | GPL1261 |
|
MouseDC-24hr-2
|
bone marrow DC, poly I:C, 24hr
|
strain: C57BL/6
tissue: bone marrow from femurs and tibias
cell type: dendritic cells
age: 3-4 months
|
Mouse bone marrow DC, Poly(I:C), 24hr, rep2
|
| Sample_geo_accession | GSM525405
| | Sample_status | Public on Aug 26 2010
| | Sample_submission_date | Mar 23 2010
| | Sample_last_update_date | Aug 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dendritic cells were harvested and plated in 60mm culture dishes at 3,000,000 DC/7 ml media and treated with Poly I:C at 2.5ug/ml for 0, 1, 3, 6, 12, or 24h. At the indicated timepoints, media was aspirated and cells were treated with RNA later (Applied Biosystems) to preserve RNA until the end of the experiment. RNA was isolated and purified using RNAqueous kit (aplied biosystems) according to manufacturer's instructions.
| | Sample_growth_protocol_ch1 | Bone marrow was isolated from the femurs and tibias of 8-10 week old C57BL/6 mice. Bone marrow cells were plated in GM-CSF-containing media for 6 days (10ng/ml). Cells were fed with cytokine containing media on days 2 and 4.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated and purified from all samples using the RNAqueous kit (Applied biolsystems) according to manufacturer instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_hyb_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_scan_protocol | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_data_processing | Affymetrix defined protocols for the GeneChip 3' IVT Express Kit were used. User's manual for these protocols can be found at www.affymetrix.com.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Elizabeth,,Hiltbold-Schwartz
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | Wake Forest University Baptist Medical Center
| | Sample_contact_address | Medical Center Boulevard
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525405/suppl/GSM525405.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM525nnn/GSM525405/suppl/GSM525405.CHP.gz
| | Sample_series_id | GSE21033
| | Sample_data_row_count | 45101
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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