Search results for the GEO ID: GSE21216  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM530531 | GPL570 |
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siASCL1_1
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ASCL1 siRNA, 72 hours
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cell line: DMS79
sirna: ASCL1
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Gene expression data from ASCL1 knock-down DMS79 cells.
DMS79_SiASCL1_1
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| Sample_geo_accession | GSM530531
| | Sample_status | Public on Jul 19 2011
| | Sample_submission_date | Apr 05 2010
| | Sample_last_update_date | Jul 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DMS79 cells were transfected with 20nM siRNA using Lipofectamine2000 (Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After cells were cultured for 72 hours, total RNA was extracted using TRIzol (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1 ug cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | 10μg cRNA, 45 degree C, 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip® scanner 3000 7G.
| | Sample_data_processing = Affymetrix Microarray Suite v5.0 (MAS5.0). Target intensity | 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | T,,Fujiwara
| | Sample_contact_email | t-fujiwara@umin.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 46-29 Yoshida Shimoadachi-cho Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM530nnn/GSM530531/suppl/GSM530531.CEL.gz
| | Sample_series_id | GSE21216
| | Sample_data_row_count | 54675
| | |
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GSM530532 | GPL570 |
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siASCL1_2
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ASCL1 siRNA, 72 hours
|
cell line: DMS79
sirna: ASCL1
|
Gene expression data from ASCL1 knock-down DMS79 cells.
DMS79_SiASCL1_2
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| Sample_geo_accession | GSM530532
| | Sample_status | Public on Jul 19 2011
| | Sample_submission_date | Apr 05 2010
| | Sample_last_update_date | Jul 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DMS79 cells were transfected with 20nM siRNA using Lipofectamine2000 (Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After cells were cultured for 72 hours, total RNA was extracted using TRIzol (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1 ug cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | 10μg cRNA, 45 degree C, 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip® scanner 3000 7G.
| | Sample_data_processing = Affymetrix Microarray Suite v5.0 (MAS5.0). Target intensity | 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | T,,Fujiwara
| | Sample_contact_email | t-fujiwara@umin.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 46-29 Yoshida Shimoadachi-cho Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM530nnn/GSM530532/suppl/GSM530532.CEL.gz
| | Sample_series_id | GSE21216
| | Sample_data_row_count | 54675
| | |
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GSM530533 | GPL570 |
|
Control
|
control, 72 hours
|
cell line: DMS79
sirna: control
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Gene expression data from ASCL1 knock-down control DMS79 cells.
DMS79_SiCont
|
| Sample_geo_accession | GSM530533
| | Sample_status | Public on Jul 19 2011
| | Sample_submission_date | Apr 05 2010
| | Sample_last_update_date | Jul 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DMS79 cells were transfected with 20nM siRNA using Lipofectamine2000 (Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After cells were cultured for 72 hours, total RNA was extracted using TRIzol (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1 ug cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | 10μg cRNA, 45 degree C, 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip® scanner 3000 7G.
| | Sample_data_processing = Affymetrix Microarray Suite v5.0 (MAS5.0). Target intensity | 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | T,,Fujiwara
| | Sample_contact_email | t-fujiwara@umin.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 46-29 Yoshida Shimoadachi-cho Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM530nnn/GSM530533/suppl/GSM530533.CEL.gz
| | Sample_series_id | GSE21216
| | Sample_data_row_count | 54675
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