Search results for the GEO ID: GSE21270  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM531792 | GPL570 |
|
FL cells, at 3 h after exposure to DMSO vehicle control, biological rep1
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 1 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531792
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531792/suppl/GSM531792.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531793 | GPL570 |
|
FL cells, at 3 h after exposure to DMSO vehicle control, biological rep2
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 1 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531793
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531793/suppl/GSM531793.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531794 | GPL570 |
|
FL cells, at 3 h after exposure to DMSO vehicle control, biological rep3
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 1 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531794
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531794/suppl/GSM531794.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531795 | GPL570 |
|
FL cells, at 3 h after exposure to 1.0 µM MNNG, biological rep1
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 1 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531795
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531795/suppl/GSM531795.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531796 | GPL570 |
|
FL cells, at 3 h after exposure to 1.0 µM MNNG, biological rep2
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 1 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531796
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531796/suppl/GSM531796.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531797 | GPL570 |
|
FL cells, at 3 h after exposure to 1.0 µM MNNG, biological rep3
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 1 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531797
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531797/suppl/GSM531797.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531798 | GPL570 |
|
FL cells, at 12 h after exposure to DMSO vehicle control, biological rep1
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 10 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531798
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531798/suppl/GSM531798.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531799 | GPL570 |
|
FL cells, at 12 h after exposure to DMSO vehicle control, biological rep2
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 10 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531799
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531799/suppl/GSM531799.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531800 | GPL570 |
|
FL cells, at 12 h after exposure to DMSO vehicle control, biological rep3
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 10 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531800
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531800/suppl/GSM531800.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531801 | GPL570 |
|
FL cells, at 12 h after exposure to 1.0 µM MNNG, biological rep1
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 10 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531801
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531801/suppl/GSM531801.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531802 | GPL570 |
|
FL cells, at 12 h after exposure to 1.0 µM MNNG, biological rep2
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 10 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531802
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531802/suppl/GSM531802.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531803 | GPL570 |
|
FL cells, at 12 h after exposure to 1.0 µM MNNG, biological rep3
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 10 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531803
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531803/suppl/GSM531803.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531804 | GPL570 |
|
FL cells, at 24 h after exposure to DMSO vehicle control, biological rep1
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 22 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531804
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531804/suppl/GSM531804.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531805 | GPL570 |
|
FL cells, at 24 h after exposure to DMSO vehicle control, biological rep2
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 22 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531805
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531805/suppl/GSM531805.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531806 | GPL570 |
|
FL cells, at 24 h after exposure to DMSO vehicle control, biological rep3
|
human amnion epithelial FL cells exposed to DMSO vehicle control for 2 h, and subsequently recovered for additional 22 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to DMSO vehicle control
|
| Sample_geo_accession | GSM531806
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531806/suppl/GSM531806.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531807 | GPL570 |
|
FL cells, at 24 h after exposure to 1.0 µM MNNG, biological rep1
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 22 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531807
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531807/suppl/GSM531807.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531808 | GPL570 |
|
FL cells, at 24 h after exposure to 1.0 µM MNNG, biological rep2
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 22 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531808
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531808/suppl/GSM531808.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
|
GSM531809 | GPL570 |
|
FL cells, at 24 h after exposure to 1.0 µM MNNG, biological rep3
|
human amnion epithelial FL cells exposed to 1.0 µM MNNG for 2 h, and subsequently recovered for additional 22 h
|
cell type: human amnion epithelial FL cells
gender: female
|
Gene expression data from FL cells exposed to 1.0 µM MNNG
|
| Sample_geo_accession | GSM531809
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Apr 08 2010
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At the logarithmic growth phase, the cultures were exposed to vehicle control (0.1%, V/V) and a low concentration (1.0 µM) MNNG for 2 h in a culture medium that did not contain 10% newborn bovine serum, followed by additional growth for 1, 10 and 22 h, respectively, in a fresh medium containing 10% newborn bovine serum. The cultures were rinsed twice with phosphate buffer solution before change of the medium.
| | Sample_growth_protocol_ch1 | Human amnion epithelial FL cells were grown as monolayers in minimal essential medium (MEM, Gibco-Invitrogen, Carlsbad, CA) supplemented with 10% newborn bovine serum (Gibco-Invitrogen), 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol reagents (Invitrogen), and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers’ protocols. Isolated total RNA was analyzed on an Agilent Bioanalyzer 2100 to verify that the starting material was of good quality.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
| | Sample_hyb_protocol | The biotinlyated cRNAs were fragmented and hybridized to the microarrays at 45°C for 16 h in the Hybridization Oven 640 rotating at 60 rpm. Following hybridization, the microarrays were washed and stained with streptavidin-phycoerythrin in the Fluidics Station 400,
| | Sample_scan_protocol | Based on the Affymetrix Microarray Suite 5.0 platform, GeneChips were scanned using the GeneChip Scanner 3000, and original Affymetrix .dat proprietary output files were generated.
| | Sample_data_processing | Microarray data analysis was conducted according to the Affymetrix Statistical Algorithms Description Document. Raw data were collected and analyzed using the Expression Console software. All transcripts spots were filtered based on spot quality, statistical test, and corrections before subsequent analyses. Data normalization with the RMA (robust multichip analysis) algorithm and differential expression analysis with ANOVA (analysis of variation) statistical method were performed by using Genomics Suite v6.5 software. Genes differentially expressed among the comparisons of the treatments and the controls were selected based upon both relative and statistical criteria (1.5-fold change cutoff; FDR (false discovery rate) <0.05; p-value<0.05 in pairwise contrasts between the treatment and the control).
| | Sample_platform_id | GPL570
| | Sample_contact_name | li,,hongjuan
| | Sample_contact_email | lihj58@sina.com
| | Sample_contact_institute | zhejiang university
| | Sample_contact_address | 353 yan'an road
| | Sample_contact_city | hangzhou
| | Sample_contact_zip/postal_code | 310058
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM531nnn/GSM531809/suppl/GSM531809.CEL.gz
| | Sample_series_id | GSE21270
| | Sample_data_row_count | 54675
| | |
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