Search results for the GEO ID: GSE21309 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM532551 | GPL1261 |
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C032 Gprc5a wild type mouse normal lung epithelial cell replicate 1
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cell line derived from trachea of three week old Gprc5a wild type mouse
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strain: C57/BL6 x sv129
cell type: lung epithelial cells from tracheas of Gprc5a wild type
age: three week old
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This sample is a normal, non-tumorigenic murine cell line derived from trachea of a three week old Gprc5a wild type mouse.
|
Sample_geo_accession | GSM532551
| Sample_status | Public on Apr 13 2010
| Sample_submission_date | Apr 12 2010
| Sample_last_update_date | Apr 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples (n=6).
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM532nnn/GSM532551/suppl/GSM532551.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
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GSM532553 | GPL1261 |
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C033 Gprc5a wild type mouse normal lung epithelial cell replicate 2
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cell line derived from trachea of three week old Gprc5a wild type mouse
|
strain: C57/BL6 x sv129
cell type: lung epithelial cells from tracheas of Gprc5a wild type
age: three week old
|
This sample is a normal, non-tumorigenic murine cell line derived from trachea of a three week old Gprc5a wild type mouse.
|
Sample_geo_accession | GSM532553
| Sample_status | Public on Apr 13 2010
| Sample_submission_date | Apr 12 2010
| Sample_last_update_date | Apr 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples (n=6).
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM532nnn/GSM532553/suppl/GSM532553.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
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GSM532555 | GPL1261 |
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C034 Gprc5a wild type mouse normal lung epithelial cell replicate 3
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cell line derived from trachea of three week old Gprc5a wild type mouse
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strain: C57/BL6 x sv129
cell type: lung epithelial cells from tracheas of Gprc5a wild type
age: three week old
|
This sample is a normal, non-tumorigenic murine cell line derived from trachea of a three week old Gprc5a wild type mouse.
|
Sample_geo_accession | GSM532555
| Sample_status | Public on Apr 13 2010
| Sample_submission_date | Apr 12 2010
| Sample_last_update_date | Apr 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples (n=6).
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM532nnn/GSM532555/suppl/GSM532555.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
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GSM532622 | GPL1261 |
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C035 Gprc5a knockout mouse normal lung epithelial cell replicate 1
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cell line derived from trachea of three week old Gprc5a knockout mouse
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strain: C57/BL6 x sv129
cell type: lung epithelial cells from tracheas of Gprc5a knockout mouse
age: three week old
|
This sample is a normal, non-tumorigenic murine cell line derived from trachea of a three week old Gprc5a knockout mouse.
|
Sample_geo_accession | GSM532622
| Sample_status | Public on Apr 13 2010
| Sample_submission_date | Apr 12 2010
| Sample_last_update_date | Apr 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples (n=6).
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM532nnn/GSM532622/suppl/GSM532622.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
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GSM532623 | GPL1261 |
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C036 Gprc5a knockout mouse normal lung epithelial cell replicate 2
|
cell line derived from trachea of three week old Gprc5a knockout mouse
|
strain: C57/BL6 x sv129
cell type: lung epithelial cells from tracheas of Gprc5a knockout mouse
age: three week old
|
This sample is a normal, non-tumorigenic murine cell line derived from trachea of a three week old Gprc5a knockout mouse.
|
Sample_geo_accession | GSM532623
| Sample_status | Public on Apr 13 2010
| Sample_submission_date | Apr 12 2010
| Sample_last_update_date | Apr 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples (n=6).
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM532nnn/GSM532623/suppl/GSM532623.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
|
GSM532624 | GPL1261 |
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C037 Gprc5a knockout mouse normal lung epithelial cell replicate 3
|
cell line derived from trachea of three week old Gprc5a knockout mouse
|
strain: C57/BL6 x sv129
cell type: lung epithelial cells from tracheas of Gprc5a knockout mouse
age: three week old
|
This sample is a normal, non-tumorigenic murine cell line derived from trachea of a three week old Gprc5a knockout mouse.
|
Sample_geo_accession | GSM532624
| Sample_status | Public on Apr 13 2010
| Sample_submission_date | Apr 12 2010
| Sample_last_update_date | Apr 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples (n=6).
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM532nnn/GSM532624/suppl/GSM532624.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
|
GSM611111 | GPL1261 |
|
C023 NNK-exposed Gprc5a knockout mouse lung adenocarcinoma cell replicate 1
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Lung adenocarcinoma cell line derived from lung tumor of NNK-exposed Gprc5a knock-out mouse
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strain: C57/BL6 x sv129
cell type: lung adenocarcinoma cells isolated and cultured from NNK-exposed Gprc5a knockout mouse
|
|
Sample_geo_accession | GSM611111
| Sample_status | Public on Oct 23 2010
| Sample_submission_date | Oct 20 2010
| Sample_last_update_date | Oct 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples.
| Sample_data_processing |
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM611nnn/GSM611111/suppl/GSM611111.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
|
GSM611112 | GPL1261 |
|
C024 NNK-exposed Gprc5a knockout mouse lung adenocarcinoma cell replicate 2
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Lung adenocarcinoma cell line derived from lung tumor of NNK-exposed Gprc5a knock-out mouse
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strain: C57/BL6 x sv129
cell type: lung adenocarcinoma cells isolated and cultured from NNK-exposed Gprc5a knockout mouse
|
|
Sample_geo_accession | GSM611112
| Sample_status | Public on Oct 23 2010
| Sample_submission_date | Oct 20 2010
| Sample_last_update_date | Oct 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples.
| Sample_data_processing |
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM611nnn/GSM611112/suppl/GSM611112.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
|
GSM611224 | GPL1261 |
|
C025 NNK-exposed Gprc5a knockout mouse lung adenocarcinoma cell replicate 3
|
Lung adenocarcinoma cell line derived from lung tumor of NNK-exposed Gprc5a knock-out mouse
|
strain: C57/BL6 x sv129
cell type: lung adenocarcinoma cells isolated and cultured from NNK-exposed Gprc5a knockout mouse
|
|
Sample_geo_accession | GSM611224
| Sample_status | Public on Oct 23 2010
| Sample_submission_date | Oct 20 2010
| Sample_last_update_date | Oct 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using RNeasy columns (Qiagen, Valencia, CA). The cells were washed twice with ice-cold PBS, lysed with the RLT buffer, incubated with DNase I for RNA isolation according to the manufacturer’s instructions. RNA quality based on the 28S/18S ribosomal RNA ratio (>1.5) was assessed using the RNA 6000 Nano Lab-On-chip and Agilent 2100 Bioanalyzer device (Agilent Technologies, Palo Alto, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNAs were synthesized by in vitro transcription (IVT) reaction using the ENZO BioArray High Yield RNA transcript labeling kit (Affymetrix, Santa Clara, CA).
| Sample_hyb_protocol | Fragmented cRNAs were then hybridized to GeneChip® Mouse Genome 430 2.0 arrays (Affymetrix), according to the manufacturer’s instructions.
| Sample_scan_protocol | The arrays were scanned with a GeneChip® Scanner 3000 from Affymetrix and raw image files were converted to probe set data (*.CEL files), using the Affymetrix GeneChip® Operating Software.
| Sample_data_processing | robust multi-chip analysis was used for normalization of this sample with other samples and with a statistical test of a p<0.01 of significance of difference across the samples.
| Sample_data_processing |
| Sample_platform_id | GPL1261
| Sample_contact_name | Humam,,Kadara
| Sample_contact_institute | University of Texas MD Anderson Cancer Center
| Sample_contact_address | 1515 Holcombe Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM611nnn/GSM611224/suppl/GSM611224.CEL.gz
| Sample_series_id | GSE21309
| Sample_data_row_count | 45101
| |
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