Search results for the GEO ID: GSE21496  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM537120 | GPL570 |
|
7-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537120
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537120/suppl/GSM537120.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537120/suppl/GSM537120.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537121 | GPL570 |
|
7-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537121
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537121/suppl/GSM537121.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537121/suppl/GSM537121.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537122 | GPL570 |
|
7-Muscle_Unloading_0h
|
vastus lateralis, control
|
tissue: vastus lateralis
protocol: control
time: 0 hour
|
|
| Sample_geo_accession | GSM537122
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537122/suppl/GSM537122.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537122/suppl/GSM537122.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537123 | GPL570 |
|
6-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537123
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537123/suppl/GSM537123.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537123/suppl/GSM537123.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537124 | GPL570 |
|
6-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537124
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537124/suppl/GSM537124.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537124/suppl/GSM537124.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537125 | GPL570 |
|
6-Muscle_Unloading_0h
|
vastus lateralis, control
|
tissue: vastus lateralis
protocol: control
time: 0 hour
|
|
| Sample_geo_accession | GSM537125
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537125/suppl/GSM537125.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537125/suppl/GSM537125.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537126 | GPL570 |
|
5-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537126
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537126/suppl/GSM537126.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537126/suppl/GSM537126.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537127 | GPL570 |
|
5-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537127
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537127/suppl/GSM537127.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537127/suppl/GSM537127.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537128 | GPL570 |
|
5-Muscle_Unloading_0h
|
vastus lateralis, control
|
tissue: vastus lateralis
protocol: control
time: 0 hour
|
|
| Sample_geo_accession | GSM537128
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537128/suppl/GSM537128.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537128/suppl/GSM537128.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537129 | GPL570 |
|
4-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537129
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537129/suppl/GSM537129.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537129/suppl/GSM537129.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537130 | GPL570 |
|
4-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537130
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537130/suppl/GSM537130.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537130/suppl/GSM537130.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537131 | GPL570 |
|
4-Muscle_Unloading_0h
|
vastus lateralis, control
|
tissue: vastus lateralis
protocol: control
time: 0 hour
|
|
| Sample_geo_accession | GSM537131
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537131/suppl/GSM537131.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537131/suppl/GSM537131.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537132 | GPL570 |
|
3-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537132
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537132/suppl/GSM537132.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537132/suppl/GSM537132.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537133 | GPL570 |
|
3-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537133
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537133/suppl/GSM537133.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537133/suppl/GSM537133.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537135 | GPL570 |
|
2-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537135
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537135/suppl/GSM537135.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537135/suppl/GSM537135.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537136 | GPL570 |
|
2-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537136
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537136/suppl/GSM537136.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537136/suppl/GSM537136.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537137 | GPL570 |
|
2-Muscle_Unloading_0h
|
vastus lateralis, control
|
tissue: vastus lateralis
protocol: control
time: 0 hour
|
|
| Sample_geo_accession | GSM537137
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537137/suppl/GSM537137.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537137/suppl/GSM537137.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537138 | GPL570 |
|
1-Muscle_Reloading_24h
|
vastus lateralis, reloading
|
tissue: vastus lateralis
protocol: reloading
time: 24 hour
|
|
| Sample_geo_accession | GSM537138
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537138/suppl/GSM537138.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537138/suppl/GSM537138.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537139 | GPL570 |
|
1-Muscle_Unloading_48h
|
vastus lateralis, unloading
|
tissue: vastus lateralis
protocol: unloading
time: 48 hour
|
|
| Sample_geo_accession | GSM537139
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537139/suppl/GSM537139.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537139/suppl/GSM537139.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
GSM537140 | GPL570 |
|
1-Muscle_Unloading_0h
|
vastus lateralis, control
|
tissue: vastus lateralis
protocol: control
time: 0 hour
|
|
| Sample_geo_accession | GSM537140
| | Sample_status | Public on Oct 23 2010
| | Sample_submission_date | Apr 23 2010
| | Sample_last_update_date | Dec 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Seven sedentary men (n = 7, mean age ± S.D | 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yi-Wen,,Chen
| | Sample_contact_email | ychen@cnmc.org
| | Sample_contact_department | Genetic Medicine Research
| | Sample_contact_institute | Children's Research Institute
| | Sample_contact_address | 111 Michigan Ave NW
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20010
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537140/suppl/GSM537140.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM537nnn/GSM537140/suppl/GSM537140.CHP.gz
| | Sample_series_id | GSE21496
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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