Search results for the GEO ID: GSE21594  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM539070 | GPL1261 |
|
ST2 cells empty vector, biological rep1
|
ST2 cells empty vector
|
age: Day 4
cell type: ST2 adipocytes
strain: BC8
|
ST2 cells empty vector, biological rep1
|
| Sample_geo_accession | GSM539070
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539070/suppl/GSM539070.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539071 | GPL1261 |
|
ST2 cells SRA overexpressed, biological rep1
|
ST2 cells SRA overexpressed
|
age: Day 4
cell type: ST2 adipocytes
strain: BC8
|
ST2 cells SRA overexpressed, biological rep1
|
| Sample_geo_accession | GSM539071
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539071/suppl/GSM539071.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539072 | GPL1261 |
|
ST2 cells empty vector, biological rep2
|
ST2 cells empty vector
|
age: Day 4
cell type: ST2 adipocytes
strain: BC8
|
ST2 cells empty vector, biological rep2
|
| Sample_geo_accession | GSM539072
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539072/suppl/GSM539072.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539073 | GPL1261 |
|
ST2 cells SRA overexpressed, biological rep2
|
ST2 cells SRA overexpressed
|
age: Day 4
cell type: ST2 adipocytes
strain: BC8
|
ST2 cells SRA overexpressed, biological rep2
|
| Sample_geo_accession | GSM539073
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539073/suppl/GSM539073.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539074 | GPL1261 |
|
ST2 cells empty vector, biological rep3
|
ST2 cells empty vector
|
age: Day 4
cell type: ST2 adipocytes
strain: BC8
|
ST2 cells empty vector, biological rep3
|
| Sample_geo_accession | GSM539074
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539074/suppl/GSM539074.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539075 | GPL1261 |
|
ST2 cells SRA overexpressed, biological rep3
|
ST2 cells SRA overexpressed
|
age: Day 4
cell type: ST2 adipocytes
strain: BC8
|
ST2 cells SRA overexpressed, biological rep3
|
| Sample_geo_accession | GSM539075
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539075/suppl/GSM539075.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539076 | GPL1261 |
|
3T3 cells empty vector, biological rep1
|
3T3 cells empty vector
|
age: Day 8
cell type: 3T3-L1 adipocytes
strain: Swiss albino
|
3T3 cells empty vector, biological rep1
|
| Sample_geo_accession | GSM539076
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539076/suppl/GSM539076.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539077 | GPL1261 |
|
3T3 cells SRA knockdown, biological rep1
|
3T3 cells SRA knockdown
|
age: Day 8
cell type: 3T3-L1 adipocytes
strain: Swiss albino
|
3T3 cells SRA knockdown, biological rep1
|
| Sample_geo_accession | GSM539077
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539077/suppl/GSM539077.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539078 | GPL1261 |
|
3T3 cells empty vector, biological rep2
|
3T3 cells empty vector
|
age: Day 8
cell type: 3T3-L1 adipocytes
strain: Swiss albino
|
3T3 cells empty vector, biological rep2
|
| Sample_geo_accession | GSM539078
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539078/suppl/GSM539078.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539079 | GPL1261 |
|
3T3 cells SRA knockdown, biological rep2
|
3T3 cells SRA knockdown
|
age: Day 8
cell type: 3T3-L1 adipocytes
strain: Swiss albino
|
3T3 cells SRA knockdown, biological rep2
|
| Sample_geo_accession | GSM539079
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539079/suppl/GSM539079.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539080 | GPL1261 |
|
3T3 cells empty vector, biological rep3
|
3T3 cells empty vector
|
age: Day 8
cell type: 3T3-L1 adipocytes
strain: Swiss albino
|
3T3 cells empty vector, biological rep3
|
| Sample_geo_accession | GSM539080
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539080/suppl/GSM539080.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
GSM539081 | GPL1261 |
|
3T3 cells SRA knockdown, biological rep3
|
3T3 cells SRA knockdown
|
age: Day 8
cell type: 3T3-L1 adipocytes
strain: Swiss albino
|
3T3 cells SRA knockdown, biological rep3
|
| Sample_geo_accession | GSM539081
| | Sample_status | Public on May 07 2013
| | Sample_submission_date | Apr 29 2010
| | Sample_last_update_date | May 07 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Induction of ST2 or 3T3-L1cell differentiation was achieved by treatment of 2 day post-confluent cells (day 0) in media supplemented with 10% FBS and a hormone cocktail containing 3-isobutyl-1-methylxanthine (0.5 mM), dexamethasone (1 μM), insulin (0.167 μM) and troglitazone (5 μM), denoted MDIT. On day 2, the cells were treated again with 0.167 μM insulin, and subsequently were refed with growth media containing 10% FBS every 2 days.
| | Sample_growth_protocol_ch1 | Mouse marrow-derived ST2 cells were incubated at 37°C in 5% CO2 in α-minimal essential medium supplemented with 10% FBS and penicillin-streptomycin. Mouse 3T3-L1 preadipocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% calf serum and penicillin-streptomycin at 37°C in 10% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the above fully (MDIT) differentiated adipocytes with Trizol reagent (Invitrogen) and cleaned up by RNeasy spin column (Qiagen Cat# 74104).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bin,,Xu
| | Sample_contact_laboratory | 5562 MSRBII
| | Sample_contact_department | Department of Internal Medicine
| | Sample_contact_institute | University of Michigan Medical School
| | Sample_contact_address | 1150 W. Medical Center Drive
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539081/suppl/GSM539081.CEL.gz
| | Sample_series_id | GSE21594
| | Sample_data_row_count | 45101
| | |
|
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