Search results for the GEO ID: GSE21610  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM545657 | GPL570 |
|
NF-1
|
Non-Failing Sample 1
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 18
gender: f
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545657
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545657/suppl/GSM545657.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545658 | GPL570 |
|
NF-3
|
Non-Failing Sample 2
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 40
gender: m
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545658
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545658/suppl/GSM545658.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545659 | GPL570 |
|
NF-7
|
Non-Failing Sample 3
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 5
gender: m
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545659
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545659/suppl/GSM545659.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545660 | GPL570 |
|
NF-8
|
Non-Failing Sample 4
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 28
gender: m
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545660
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545660/suppl/GSM545660.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545661 | GPL570 |
|
NF-9
|
Non-Failing Sample 5
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 64
gender: m
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545661
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545661/suppl/GSM545661.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545662 | GPL570 |
|
NF-10
|
Non-Failing Sample 6
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 31
gender: m
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545662
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545662/suppl/GSM545662.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545663 | GPL570 |
|
NF-11
|
Non-Failing Sample 7
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 23
gender: m
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545663
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545663/suppl/GSM545663.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545664 | GPL570 |
|
NF-14
|
Non-Failing Sample 8
|
sample pairs: none
tissue: Myocard
vad status: unsupported
disease status: none
vad: none
age: 23
gender: f
|
Gene expression data from non-failing control hearts
|
| Sample_geo_accession | GSM545664
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545664/suppl/GSM545664.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545665 | GPL570 |
|
VAD-IP-2
|
Implant Sample 1
|
sample pairs: 2
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 32
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545665
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545665/suppl/GSM545665.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545666 | GPL570 |
|
VAD-IP-3
|
Implant Sample 2
|
sample pairs: 3
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Thoratec
age: 40
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545666
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545666/suppl/GSM545666.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545667 | GPL570 |
|
VAD-IP-4
|
Implant Sample 3
|
sample pairs: 4
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 59
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545667
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545667/suppl/GSM545667.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545668 | GPL570 |
|
VAD-IP-6
|
Implant Sample 4
|
sample pairs: 6
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 44
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545668
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545668/suppl/GSM545668.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545669 | GPL570 |
|
VAD-IP-7
|
Implant Sample 5
|
sample pairs: 7
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Thoratec (iVAD)
age: 59
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545669
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545669/suppl/GSM545669.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545670 | GPL570 |
|
VAD-IP-8
|
Implant Sample 6
|
sample pairs: 8
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Heartmate I
age: 52
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545670
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545670/suppl/GSM545670.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545671 | GPL570 |
|
VAD-IP-9
|
Implant Sample 7
|
sample pairs: 9
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 60
gender: f
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545671
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545671/suppl/GSM545671.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545672 | GPL570 |
|
VAD-IP-10
|
Implant Sample 8
|
sample pairs: 10
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Novacor
age: 66
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545672
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545672/suppl/GSM545672.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545673 | GPL570 |
|
VAD-IP-12
|
Implant Sample 9
|
sample pairs: 12
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: CorAide
age: 65
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545673
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545673/suppl/GSM545673.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545674 | GPL570 |
|
VAD-IP-14
|
Implant Sample 10
|
sample pairs: 14
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: DuraHeart
age: 55
gender: f
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545674
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545674/suppl/GSM545674.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545675 | GPL570 |
|
VAD-IP-15
|
Implant Sample 11
|
sample pairs: 15
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Heartmate I
age: 67
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545675
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545675/suppl/GSM545675.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545676 | GPL570 |
|
VAD-IP-16
|
Implant Sample 12
|
sample pairs: 16
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Novacor
age: 52
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545676
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545676/suppl/GSM545676.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545677 | GPL570 |
|
VAD-IP-17
|
Implant Sample 13
|
sample pairs: 17
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: DuraHeart
age: 44
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545677
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545677/suppl/GSM545677.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545678 | GPL570 |
|
VAD-IP-18
|
Implant Sample 14
|
sample pairs: 18
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Thoratec
age: 63
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545678
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545678/suppl/GSM545678.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545679 | GPL570 |
|
VAD-IP-21
|
Implant Sample 15
|
sample pairs: 21
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Novacor
age: 63
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545679
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545679/suppl/GSM545679.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545680 | GPL570 |
|
VAD-IP-22
|
Implant Sample 16
|
sample pairs: 22
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Incor
age: 43
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545680
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545680/suppl/GSM545680.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545681 | GPL570 |
|
VAD-IP-23
|
Implant Sample 17
|
sample pairs: 23
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 16
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545681
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545681/suppl/GSM545681.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545682 | GPL570 |
|
VAD-IP-24
|
Implant Sample 18
|
sample pairs: 24
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 56
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545682
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545682/suppl/GSM545682.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545683 | GPL570 |
|
VAD-IP-26
|
Implant Sample 19
|
sample pairs: 26
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: DuraHeart
age: 40
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545683
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545683/suppl/GSM545683.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545684 | GPL570 |
|
VAD-IP-27
|
Implant Sample 20
|
sample pairs: 27
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 59
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545684
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545684/suppl/GSM545684.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545685 | GPL570 |
|
VAD-IP-28
|
Implant Sample 21
|
sample pairs: 28
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 45
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545685
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545685/suppl/GSM545685.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545686 | GPL570 |
|
VAD-IP-29
|
Implant Sample 22
|
sample pairs: 29
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 58
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545686
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545686/suppl/GSM545686.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545687 | GPL570 |
|
VAD-IP-30
|
Implant Sample 23
|
sample pairs: 30
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: DuraHeart
age: 68
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545687
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545687/suppl/GSM545687.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545688 | GPL570 |
|
VAD-IP-40
|
Implant Sample 24
|
sample pairs: 40
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 47
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545688
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545688/suppl/GSM545688.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545689 | GPL570 |
|
VAD-IP-42
|
Implant Sample 25
|
sample pairs: 42
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy (VD)
vad: Heartmate I
age: 33
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545689
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545689/suppl/GSM545689.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545690 | GPL570 |
|
VAD-IP-44
|
Implant Sample 26
|
sample pairs: 44
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Thoratec (biVAD)
age: 50
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545690
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545690/suppl/GSM545690.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545691 | GPL570 |
|
VAD-IP-45
|
Implant Sample 27
|
sample pairs: 45
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: Heartmate I
age: 57
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545691
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545691/suppl/GSM545691.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545692 | GPL570 |
|
VAD-IP-48
|
Implant Sample 28
|
sample pairs: 48
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 66
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545692
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545692/suppl/GSM545692.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545693 | GPL570 |
|
VAD-IP-51
|
Implant Sample 29
|
sample pairs: 51
tissue: Myocard
vad status: before VAD support
disease status: dilated cardiomyopathy
vad: CorAide
age: 29
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545693
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545693/suppl/GSM545693.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545694 | GPL570 |
|
VAD-IP-57
|
Implant Sample 30
|
sample pairs: 57
tissue: Myocard
vad status: before VAD support
disease status: ischemic cardiomyopathy
vad: DuraHeart
age: 50
gender: m
|
Gene expression data from patients suffering end-stage heart-failure
|
| Sample_geo_accession | GSM545694
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545694/suppl/GSM545694.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545695 | GPL570 |
|
VAD-HTx-2
|
Transplant Sample 1
|
sample pairs: 2
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 32
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545695
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545695/suppl/GSM545695.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545696 | GPL570 |
|
VAD-HTx-3
|
Transplant Sample 2
|
sample pairs: 3
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Thoratec
age: 40
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545696
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545696/suppl/GSM545696.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545697 | GPL570 |
|
VAD-HTx-4
|
Transplant Sample 3
|
sample pairs: 4
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 59
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545697
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545697/suppl/GSM545697.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545698 | GPL570 |
|
VAD-HTx-6
|
Transplant Sample 4
|
sample pairs: 6
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 44
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545698
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545698/suppl/GSM545698.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545699 | GPL570 |
|
VAD-HTx-7
|
Transplant Sample 5
|
sample pairs: 7
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Thoratec (iVAD)
age: 59
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545699
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545699/suppl/GSM545699.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545700 | GPL570 |
|
VAD-HTx-8
|
Transplant Sample 6
|
sample pairs: 8
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Heartmate I
age: 52
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545700
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545700/suppl/GSM545700.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545701 | GPL570 |
|
VAD-HTx-9
|
Transplant Sample 7
|
sample pairs: 9
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 60
gender: f
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545701
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545701/suppl/GSM545701.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545702 | GPL570 |
|
VAD-HTx-10
|
Transplant Sample 8
|
sample pairs: 10
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Novacor
age: 66
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545702
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545702/suppl/GSM545702.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545703 | GPL570 |
|
VAD-HTx-12
|
Transplant Sample 9
|
sample pairs: 12
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: CorAide
age: 65
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545703
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545703/suppl/GSM545703.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545704 | GPL570 |
|
VAD-HTx-14
|
Transplant Sample 10
|
sample pairs: 14
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: DuraHeart
age: 55
gender: f
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545704
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545704/suppl/GSM545704.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545705 | GPL570 |
|
VAD-HTx-15
|
Transplant Sample 11
|
sample pairs: 15
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Heartmate I
age: 67
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545705
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545705/suppl/GSM545705.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545706 | GPL570 |
|
VAD-HTx-16
|
Transplant Sample 12
|
sample pairs: 16
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Novacor
age: 52
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545706
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545706/suppl/GSM545706.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545707 | GPL570 |
|
VAD-HTx-17
|
Transplant Sample 13
|
sample pairs: 17
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: DuraHeart
age: 44
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545707
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545707/suppl/GSM545707.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545708 | GPL570 |
|
VAD-HTx-18
|
Transplant Sample 14
|
sample pairs: 18
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Thoratec
age: 63
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545708
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545708/suppl/GSM545708.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545709 | GPL570 |
|
VAD-HTx-21
|
Transplant Sample 15
|
sample pairs: 21
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Novacor
age: 63
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545709
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545709/suppl/GSM545709.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545710 | GPL570 |
|
VAD-HTx-22
|
Transplant Sample 16
|
sample pairs: 22
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Incor
age: 43
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545710
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545710/suppl/GSM545710.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545711 | GPL570 |
|
VAD-HTx-23
|
Transplant Sample 17
|
sample pairs: 23
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 16
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545711
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545711/suppl/GSM545711.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545712 | GPL570 |
|
VAD-HTx-24
|
Transplant Sample 18
|
sample pairs: 24
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 56
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545712
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545712/suppl/GSM545712.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545713 | GPL570 |
|
VAD-HTx-26
|
Transplant Sample 19
|
sample pairs: 26
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: DuraHeart
age: 40
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545713
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545713/suppl/GSM545713.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545714 | GPL570 |
|
VAD-HTx-27
|
Transplant Sample 20
|
sample pairs: 27
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 59
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545714
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545714/suppl/GSM545714.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545715 | GPL570 |
|
VAD-HTx-28
|
Transplant Sample 21
|
sample pairs: 28
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 45
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545715
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545715/suppl/GSM545715.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545716 | GPL570 |
|
VAD-HTx-29
|
Transplant Sample 22
|
sample pairs: 29
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 58
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545716
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545716/suppl/GSM545716.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545717 | GPL570 |
|
VAD-HTx-30
|
Transplant Sample 23
|
sample pairs: 30
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: DuraHeart
age: 68
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545717
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545717/suppl/GSM545717.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545718 | GPL570 |
|
VAD-HTx-40
|
Transplant Sample 24
|
sample pairs: 40
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Heartmate I
age: 47
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545718
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545718/suppl/GSM545718.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545719 | GPL570 |
|
VAD-HTx-42
|
Transplant Sample 25
|
sample pairs: 42
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy (VD)
vad: Heartmate I
age: 33
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545719
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545719/suppl/GSM545719.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545720 | GPL570 |
|
VAD-HTx-44
|
Transplant Sample 26
|
sample pairs: 44
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Thoratec (biVAD)
age: 50
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545720
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545720/suppl/GSM545720.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545721 | GPL570 |
|
VAD-HTx-45
|
Transplant Sample 27
|
sample pairs: 45
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: Heartmate I
age: 57
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545721
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545721/suppl/GSM545721.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545722 | GPL570 |
|
VAD-HTx-48
|
Transplant Sample 28
|
sample pairs: 48
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: Novacor
age: 66
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545722
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545722/suppl/GSM545722.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545723 | GPL570 |
|
VAD-HTx-51
|
Transplant Sample 29
|
sample pairs: 51
tissue: Myocard
vad status: after VAD support
disease status: dilated cardiomyopathy
vad: CorAide
age: 29
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545723
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545723/suppl/GSM545723.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
|
GSM545724 | GPL570 |
|
VAD-HTx-57
|
Transplant Sample 30
|
sample pairs: 57
tissue: Myocard
vad status: after VAD support
disease status: ischemic cardiomyopathy
vad: DuraHeart
age: 50
gender: m
|
Gene expression data from patients suffering end-stage heart-failure after VAD support
|
| Sample_geo_accession | GSM545724
| | Sample_status | Public on May 20 2010
| | Sample_submission_date | May 20 2010
| | Sample_last_update_date | May 20 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples were frozen in liquid nitrogen directly after pruning and defrosted only for microarray analysis.
| | Sample_growth_protocol_ch1 | Myocardial tissue was directly taken from non-failing and failing hearts.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Samples were pruned from the left ventricle near the apex. RNA extraction has been carried out with TRIzol reagent and tissue was homogenized using a rotor-stator homogenizer. Homogenates were transferred to a Phase Lock Gel (PLG) Heavy tube (Eppendorf), chloroform was added, and the mixture was centrifuged. Supernatants were collected, and isopropanol was added to precipitate the RNA. The RNA pellet was washed with 80% ethanol, then air-dried before dissolving in 100 l of RNase-free H2O. RNA was further purified using the RNeasy Mini protocol according to the manufacturer’s directions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Patrick,,Schwientek
| | Sample_contact_department | Klinik f. Thorax- & Kardiovaskularchirurgie
| | Sample_contact_institute | Herz- & Diabeteszentrum NRW
| | Sample_contact_address | Georgstr. 11
| | Sample_contact_city | Bad Oeynhausen
| | Sample_contact_zip/postal_code | 32545
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545724/suppl/GSM545724.cel.gz
| | Sample_series_id | GSE21610
| | Sample_data_row_count | 54675
| | |
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