Search results for the GEO ID: GSE21612  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM539491 | GPL570 |
|
cultured parental, biological rep 1
|
cultured parental cells
|
cell line: NCI-H1299
medium: serum-containing medium
condition: cultured
|
NCI-H1299_P1
|
| Sample_geo_accession | GSM539491
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539491/suppl/GSM539491.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539492 | GPL570 |
|
cultured parental, biological rep 2
|
cultured parental cells
|
cell line: NCI-H1299
medium: serum-containing medium
condition: cultured
|
NCI-H1299_P2
|
| Sample_geo_accession | GSM539492
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539492/suppl/GSM539492.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539493 | GPL570 |
|
cultured parental, biological rep 3
|
cultured parental cells
|
cell line: NCI-H1299
medium: serum-containing medium
condition: cultured
|
NCI-H1299_P3
|
| Sample_geo_accession | GSM539493
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539493/suppl/GSM539493.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539494 | GPL570 |
|
cultured spheroid, biological rep 1
|
cultured spheroid cells
|
cell line: NCI-H1299
medium: stem-cell medium
condition: cultured
|
NCI-H1299_S1
|
| Sample_geo_accession | GSM539494
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539494/suppl/GSM539494.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539495 | GPL570 |
|
cultured spheroid, biological rep 2
|
cultured spheroid cells
|
cell line: NCI-H1299
medium: stem-cell medium
condition: cultured
|
NCI-H1299_S2
|
| Sample_geo_accession | GSM539495
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539495/suppl/GSM539495.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539496 | GPL570 |
|
cultured spheroid, biological rep 3
|
cultured spheroid cells
|
cell line: NCI-H1299
medium: stem-cell medium
condition: cultured
|
NCI-H1299_S3
|
| Sample_geo_accession | GSM539496
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539496/suppl/GSM539496.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539497 | GPL570 |
|
xenograft parental, biological rep 1
|
xenografted parental cells
|
cell line: NCI-H1299
medium: serum-containing medium
condition: xenografted
|
NCI-H1299_X_P1
|
| Sample_geo_accession | GSM539497
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539497/suppl/GSM539497.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539498 | GPL570 |
|
xenograft parental, biological rep 2
|
xenografted parental cells
|
cell line: NCI-H1299
medium: serum-containing medium
condition: xenografted
|
NCI-H1299_X_P2
|
| Sample_geo_accession | GSM539498
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539498/suppl/GSM539498.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539499 | GPL570 |
|
xenograft parental, biological rep 3
|
xenografted parental cells
|
cell line: NCI-H1299
medium: serum-containing medium
condition: xenografted
|
NCI-H1299_X_P3
|
| Sample_geo_accession | GSM539499
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539499/suppl/GSM539499.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539500 | GPL570 |
|
xenograft spheroid, biological rep 1
|
xenografted spheroid cells
|
cell line: NCI-H1299
medium: stem-cell medium
condition: xenografted
|
NCI-H1299_X_S1
|
| Sample_geo_accession | GSM539500
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539500/suppl/GSM539500.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
| | |
|
GSM539501 | GPL570 |
|
xenograft spheroid, biological rep 2
|
xenografted spheroid cells
|
cell line: NCI-H1299
medium: stem-cell medium
condition: xenografted
|
NCI-H1299_X_S2
|
| Sample_geo_accession | GSM539501
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539501/suppl/GSM539501.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
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GSM539502 | GPL570 |
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xenograft spheroid, biological rep 3
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xenografted spheroid cells
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cell line: NCI-H1299
medium: stem-cell medium
condition: xenografted
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NCI-H1299_X_S3
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| Sample_geo_accession | GSM539502
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Apr 30 2010
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To generate spheroid cultures, exponentially growing NCI-H1299 monolayer cultures were changed into the CSC medium. After 7-10 days of continual cultivation with addition of fresh culture medium every other day, the cells in suspension were collected and plated in ultra low attachment flasks (Corning, Corning, NY).
| | Sample_growth_protocol_ch1 | A non-small cell lung cancer cell line NCI-NCI-H1299, purchased from the ATCC (Manassas, VA), was cultured at 37 C, 5% CO2 in humidified atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy kit (Qiagen, Germantown, MD) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For microarray sample processing, 2 µg of total RNA was used to synthesize cRNA using Affymetrix IVT one-cycle labeling kit per manufacturer's protocol.
| | Sample_hyb_protocol | Fifteen micrograms of labeled, fragmented cRNA was hybridized to Affymetrix HGU133 Plus 2.0 array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were RMA normalized using Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Douglas Fang,,Fang
| | Sample_contact_email | Douglas.Fang@pfizer.com
| | Sample_contact_department | Oncology Research Unit
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | 10777 Science Center Drive, CB5
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM539nnn/GSM539502/suppl/GSM539502.CEL.gz
| | Sample_series_id | GSE21612
| | Sample_data_row_count | 54675
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