Search results for the GEO ID: GSE21654  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM540374 | GPL570 |
|
Pancreatic Cancer Cell Line Nor-P1
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Nor-P1
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540374
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540374/suppl/GSM540374.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540375 | GPL570 |
|
Pancreatic Cancer Cell Line HPAF-II
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: HPAF-II
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540375
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540375/suppl/GSM540375.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540376 | GPL570 |
|
Pancreatic Cancer Cell Line CaPan-2
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: CaPan-2
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540376
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540376/suppl/GSM540376.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540377 | GPL570 |
|
Pancreatic Cancer Cell Line BxPC-3
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: BxPC-3
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540377
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540377/suppl/GSM540377.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540378 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 2.03
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 2.03
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540378
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540378/suppl/GSM540378.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540379 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 5.04
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 5.04
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540379
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540379/suppl/GSM540379.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540380 | GPL570 |
|
Pancreatic Cancer Cell Line SU86.86
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: SU86.86
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540380
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540380/suppl/GSM540380.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540381 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 3.27
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 3.27
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540381
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540381/suppl/GSM540381.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540382 | GPL570 |
|
Pancreatic Cancer Cell Line PL45
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: PL45
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540382
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540382/suppl/GSM540382.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540383 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 3.3
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 3.3
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540383
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540383/suppl/GSM540383.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540384 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 6.03
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 6.03
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540384
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540384/suppl/GSM540384.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540385 | GPL570 |
|
Pancreatic Cancer Cell Line CFPAC
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: CFPAC
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540385
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540385/suppl/GSM540385.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540386 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 10.05
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 10.05
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540386
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540386/suppl/GSM540386.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540387 | GPL570 |
|
Pancreatic Cancer Cell Line Colo 357
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Colo 357
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540387
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540387/suppl/GSM540387.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540388 | GPL570 |
|
Pancreatic Cancer Cell Line L3.6 pl
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: L3.6 pl
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540388
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540388/suppl/GSM540388.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540389 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 2.13
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 2.13
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540389
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540389/suppl/GSM540389.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540390 | GPL570 |
|
Pancreatic Cancer Cell Line ASPC-1
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: ASPC-1
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540390
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540390/suppl/GSM540390.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540391 | GPL570 |
|
Pancreatic Cancer Cell Line Panc 8.13
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc 8.13
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540391
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540391/suppl/GSM540391.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540392 | GPL570 |
|
Pancreatic Cancer Cell Line Mpanc-96
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Mpanc-96
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540392
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540392/suppl/GSM540392.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540393 | GPL570 |
|
Pancreatic Cancer Cell Line HS766t
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: HS766t
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540393
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540393/suppl/GSM540393.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
| | |
|
GSM540394 | GPL570 |
|
Pancreatic Cancer Cell Line MiaPaCa-2
|
Pancreatic Tumor
|
tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: MiaPaCa-2
|
Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540394
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540394/suppl/GSM540394.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
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GSM540395 | GPL570 |
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Pancreatic Cancer Cell Line Panc-1
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Pancreatic Tumor
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tissue: Pancreas
cell type: Pancreatic Cancer Cell Line
cell line: Panc-1
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Gene expression data from untreated cells
|
| Sample_geo_accession | GSM540395
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | May 04 2010
| | Sample_last_update_date | May 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The pancreatic cancer cell lines were grown to 80% confluence in DMEM/FBS/PenStrep media and cell pellets isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the cell pellets through homogenization in TRIzol (Invitrogen, Stockholm, Sweden) using a conventional rotor-stator homogenizer. Nucleic acid was further separated through centrifugation in the presence of chloroform. RNA was then precipitated with isopropyl alcohol and washed with 75% alcohol. RNA pellets were resuspended in nuclease free water. The RNA was further purified using the silica-gel membrane-based spin-column technology RNeasy Mini-/Micro-KitTM (Qiagen, Valencia, CA). RNA quantity was determined using a Nanodrop Spectrophotometer, and RNA quality was determined using the Agilent 2100 Bioanalyzer lab-on-a-chip technology (Agilent, Foster City, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled one round cRNA was generated using kits (Gene-Chip One-Cycle Target Labeling and Control Reagent) from Affymetrix.
| | Sample_hyb_protocol | Standard Affymetrix Protocol
| | Sample_scan_protocol | Standard Affymetrix protocol using GeneChip Scanner 3000 7G with auto loader
| | Sample_data_processing | The data were normalized using Affymetrix Microarray Suite 5.0 using the global scaling technique. The 95% trimmed mean was set arbitrarily to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | brian,,haab
| | Sample_contact_email | brian.haab@vai.org
| | Sample_contact_phone | 6162345268
| | Sample_contact_department | lab of cancer immunodiagnostics
| | Sample_contact_institute | van andel institute
| | Sample_contact_address | 333 bostwick avenue NE
| | Sample_contact_city | grand rapids
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 49503
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540395/suppl/GSM540395.CEL.gz
| | Sample_series_id | GSE21654
| | Sample_data_row_count | 54675
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