Search results for the GEO ID: GSE21683  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM540903 | GPL1261 |
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Cdk4+/+:Eµ-Myc B-lymphoma
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Cdk4+/+:Eµ-Myc B-lymphoma
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cell type: B-lymphoma cell
genotype: Cdk4+/+:Eµ-Myc
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mice lymph nodes
Gene expression data from Cdk4+/+:Eµ-Myc B-lymphoma
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| Sample_geo_accession | GSM540903
| | Sample_status | Public on Dec 16 2012
| | Sample_submission_date | May 05 2010
| | Sample_last_update_date | Dec 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | Cell lines and isolating the B-lymphoma cells form the cdk4+/+ Eµ-myc and cdk4-/-Eµ-myc mice culture in 45% Iscove’s modified Eagle medium, 45% Dulbecco’s minimal essential medium, 10% fetal bovine serum, 100 U/ml penicillin and streptomycin, 4 mM L-glutamine, and 50 μM 2-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Xianghong,,Zou
| | Sample_contact_email | zou.32@osu.edu
| | Sample_contact_phone | 614-688-8424
| | Sample_contact_fax | 614-292-7072
| | Sample_contact_department | Pathology
| | Sample_contact_institute | Ohoi State University
| | Sample_contact_address | 1645 Neil Ave
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540903/suppl/GSM540903_Bplus.CEL.gz
| | Sample_series_id | GSE21683
| | Sample_data_row_count | 45101
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GSM540904 | GPL1261 |
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Cdk4-/-:Eµ-Myc B-lymphoma
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Cdk4-/-:Eµ-Myc B-lymphoma
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cell type: B-lymphoma cell
genotype: Cdk4-/-:Eµ-Myc
|
mice lymph nodes
Gene expression data from Cdk4-/-:Eµ-Myc B-lymphoma
|
| Sample_geo_accession | GSM540904
| | Sample_status | Public on Dec 16 2012
| | Sample_submission_date | May 05 2010
| | Sample_last_update_date | Dec 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | Cell lines and isolating the B-lymphoma cells form the cdk4+/+ Eµ-myc and cdk4-/-Eµ-myc mice culture in 45% Iscove’s modified Eagle medium, 45% Dulbecco’s minimal essential medium, 10% fetal bovine serum, 100 U/ml penicillin and streptomycin, 4 mM L-glutamine, and 50 μM 2-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Xianghong,,Zou
| | Sample_contact_email | zou.32@osu.edu
| | Sample_contact_phone | 614-688-8424
| | Sample_contact_fax | 614-292-7072
| | Sample_contact_department | Pathology
| | Sample_contact_institute | Ohoi State University
| | Sample_contact_address | 1645 Neil Ave
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM540nnn/GSM540904/suppl/GSM540904_Bminus.CEL.gz
| | Sample_series_id | GSE21683
| | Sample_data_row_count | 45101
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