Search results for the GEO ID: GSE21700  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM541601 | GPL1261 |
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Ebf2-lacZ-expressing osteoblastic cells from Ebf2+/- mice
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Osteoblastic cell preparation, sorted for lacZ Marker (Ebf2-lacZ; Kieslinger et al., 2006), expanded in culture without passaging.
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strain: C57Bl/6
developmental stage: E18.5 embryo
genotype/variation: Ebf2+/-
cell type: osteoblast preparation expanded in culture without passaging
tissue: tibia, femur
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osteoblastic cells from tibia and femur of Ebf2 heterozygous mice (n=3 each) were loaded with FDG, FACS sorted and total RNA was isolated. cDNA was synthesised using the One-Cycle cDNA Synthesis Kit (Affymetrix), and labeled cRNA was synthesized by transcription in vitro, using the IVT labelling kit (Affymetrix). The labeled RNA was fragmented and hybridized to GeneChip® Mouse genome 430 2.0 arrays (GPL1261), according to the manufacturer’s instructions.
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| Sample_geo_accession | GSM541601
| | Sample_status | Public on Apr 26 2011
| | Sample_submission_date | May 05 2010
| | Sample_last_update_date | Apr 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Matthias Kieslinger, Laboratory Prof. Rudolf Grosschedl Feodor-Lynen-Strasse 25 81377 Munich Germany
| | Sample_growth_protocol_ch1 | alpha MEM supplemented with 10% FCS, Penicillin/Streptomycin and L-Glutamine
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was preparedwith Trizol reagent (Invitrogen) according to the manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | IVT Labeling Kit (Affymetrix, P/N 900449)
| | Sample_hyb_protocol | performed according to the GeneChip® Expression Analysis Technical Manual
| | Sample_scan_protocol | performed according to the GeneChip® Expression Analysis Technical Manual
| | Sample_scan_protocol | (GeneChip Scanner 3000)
| | Sample_data_processing | Data analysis was done with the GeneChip Operating Software using the MAS5 algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Rudolf ,,Grosschedl
| | Sample_contact_email | grosschedl@immunbio.mpg.de
| | Sample_contact_institute | Max-Planck Institute of Immunobiology
| | Sample_contact_address | Stübeweg 51
| | Sample_contact_city | Freiburg
| | Sample_contact_zip/postal_code | 79108
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM541nnn/GSM541601/suppl/GSM541601.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM541nnn/GSM541601/suppl/GSM541601.CHP.gz
| | Sample_series_id | GSE21700
| | Sample_data_row_count | 45101
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GSM541611 | GPL1261 |
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Ebf2-lacZ-expressing osteoblastic cells from Ebf2-/- mice
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Osteoblastic cell preparation, sorted for lacZ Marker (Ebf2-lacZ; Kieslinger et al., 2006), expanded in culture without passaging.
|
strain: C57Bl/6
developmental stage: E18.5 embryo
genotype/variation: Ebf2-/-
cell type: osteoblast preparation expanded in culture without passaging
tissue: tibia, femur
|
osteoblastic cells from tibia and femur of Ebf2 knockout mice (n=3 each) were loaded with FDG, FACS sorted and total RNA was isolated. cDNA was synthesised using the One-Cycle cDNA Synthesis Kit (Affymetrix), and labeled cRNA was synthesized by transcription in vitro, using the IVT labelling kit (Affymetrix). The labeled RNA was fragmented and hybridized to GeneChip® Mouse genome 430 2.0 arrays (GPL1261), according to the manufacturer’s instructions.
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| Sample_geo_accession | GSM541611
| | Sample_status | Public on Apr 26 2011
| | Sample_submission_date | May 05 2010
| | Sample_last_update_date | Apr 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Matthias Kieslinger, Laboratory Prof. Rudolf Grosschedl Feodor-Lynen-Strasse 25 81377 Munich Germany
| | Sample_growth_protocol_ch1 | alpha MEM supplemented with 10% FCS, Penicillin/Streptomycin and L-Glutamine
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was prepared with Trizol reagent (Invitrogen) according to the manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | IVT Labeling Kit (Affymetrix, P/N 900449)
| | Sample_hyb_protocol | performed according to the GeneChip® Expression Analysis Technical Manual
| | Sample_scan_protocol | performed according to the GeneChip® Expression Analysis Technical Manual
| | Sample_scan_protocol | (GeneChip Scanner 3000)
| | Sample_data_processing | Data analysis was done with the GeneChip Operating Software using the MAS5 algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Rudolf ,,Grosschedl
| | Sample_contact_email | grosschedl@immunbio.mpg.de
| | Sample_contact_institute | Max-Planck Institute of Immunobiology
| | Sample_contact_address | Stübeweg 51
| | Sample_contact_city | Freiburg
| | Sample_contact_zip/postal_code | 79108
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM541nnn/GSM541611/suppl/GSM541611.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM541nnn/GSM541611/suppl/GSM541611.CHP.gz
| | Sample_series_id | GSE21700
| | Sample_data_row_count | 45101
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