Search results for the GEO ID: GSE21754  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM542344 | GPL1261 |
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Control_WT_mice_WAT
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C57BL/6 mice, white adipose tissue
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genetic background: C57BL/6
gender: female
age: 30-week-old
tissue: perigonadal white adipose tissue
genotype: wild type
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Gene expression data from perigonadal white adipose tissue in HFD-fed wildtype C57BL/6 mice.
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| Sample_geo_accession | GSM542344
| | Sample_status | Public on May 13 2010
| | Sample_submission_date | May 08 2010
| | Sample_last_update_date | May 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed by using Ambion Ribopure Kit (Cat.# AM1924), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hideaki,,Miyoshi
| | Sample_contact_department | Internal Medicine II
| | Sample_contact_institute | Hokkaido University Graduate School of Medicine
| | Sample_contact_address | Kita-15, Nishi-7, Kita-ku
| | Sample_contact_city | Sapporo
| | Sample_contact_state | Hokkaido
| | Sample_contact_zip/postal_code | 060-8638
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.med.hokudai.ac.jp/~med-2w/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542344/suppl/GSM542344.CEL.gz
| | Sample_series_id | GSE21754
| | Sample_data_row_count | 45101
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GSM542345 | GPL1261 |
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PeriA_Tg_mice_WAT
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PeriA transgenic mice, white adipose tissue
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genetic background: C57BL/6
gender: female
age: 30-week-old
tissue: perigonadal white adipose tissue
genotype: PeriA transgeneic
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Gene expression data from perigonadal white adipose tissue in HFD-fed PeriA transgenic mice. The mice are overexpressed human PeriA using the adipocyte specific aP2 promoter/enhancer (Miyoshi, et al. J Lipid Res 2010).
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| Sample_geo_accession | GSM542345
| | Sample_status | Public on May 13 2010
| | Sample_submission_date | May 08 2010
| | Sample_last_update_date | May 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed by using Ambion Ribopure Kit (Cat.# AM1924), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hideaki,,Miyoshi
| | Sample_contact_department | Internal Medicine II
| | Sample_contact_institute | Hokkaido University Graduate School of Medicine
| | Sample_contact_address | Kita-15, Nishi-7, Kita-ku
| | Sample_contact_city | Sapporo
| | Sample_contact_state | Hokkaido
| | Sample_contact_zip/postal_code | 060-8638
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.med.hokudai.ac.jp/~med-2w/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542345/suppl/GSM542345.CEL.gz
| | Sample_series_id | GSE21754
| | Sample_data_row_count | 45101
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