Result

Search results for the GEO ID: GSE21836

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM543423

GPL1261

Liver_normal_0hr_rep1

Liver, normal, 0hr

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Wild Type

Gene expression data from normal mouse liver at 0hr

Sample_geo_accession	GSM543423
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543423/suppl/GSM543423.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543424

GPL1261

Liver_normal_0hr_rep2

Liver, normal, 0hr

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Wild Type

Gene expression data from normal mouse liver at 0hr

Sample_geo_accession	GSM543424
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543424/suppl/GSM543424.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543425

GPL1261

Liver_normal_24hr_rep1

Liver, normal, 24hr after 2/3 hepatectomy

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Wild Type

Gene expression data from normal mouse liver at 24hr. After 2/3 hepatoctamy

Sample_geo_accession	GSM543425
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543425/suppl/GSM543425.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543426

GPL1261

Liver_normal_24hr_rep2

Liver, normal, 24hr after 2/3 hepatectomy

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Wild Type

Gene expression data from normal mouse liver at 24hr. After 2/3 hepatoctamy

Sample_geo_accession	GSM543426
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543426/suppl/GSM543426.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543427

GPL1261

Liver_Tob1KO_0hr_rep1

Liver, Tob1 null, 0hr

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Tob1 null

Gene expression data from Tob1 null mouse liver at 0hr

Sample_geo_accession	GSM543427
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543427/suppl/GSM543427.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543428

GPL1261

Liver_Tob1KO_0hr_rep2

Liver, Tob1 null, 0hr

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Tob1 null

Gene expression data from Tob1 null mouse liver at 0hr

Sample_geo_accession	GSM543428
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543428/suppl/GSM543428.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543429

GPL1261

Liver_Tob1KO_24hr_rep1

Liver, Tob1 null, 24hr after 2/3 hepatectomy

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Tob1 null

Gene expression data from Tob1 null mouse liver at 24hr. After 2/3 hepatoctamy

Sample_geo_accession	GSM543429
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543429/suppl/GSM543429.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

GSM543430

GPL1261

Liver_Tob1KO_24hr_rep2

Liver, Tob1 null, 24hr after 2/3 hepatectomy

gender: male age: 7-10 weeks tissue: Liver strain: C57BL/6 genotype/variation: Tob1 null

Gene expression data from Tob1 null mouse liver at 24hr. After 2/3 hepatoctamy

Sample_geo_accession	GSM543430
Sample_status	Public on May 15 2010
Sample_submission_date	May 14 2010
Sample_last_update_date	May 14 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	All surgeries were performed according to the National Institutes of Health guidelines for the humane treatment of laboratory animals and with approval of the Harvard Institutional Animal Care and Use Committee. Mice mice were anesthetized with 60mg/kg ketamine (Hospira) and 7mg/kg xylazine (Phoenix Pharmaceuticals), and positioned supine. A transverse incision was made inferior to the xiphoid process, which was excised. The median and left lateral lobes of the liver were eviscerated and ligated. At tissue recovery, mice were anesthetized and weighed. Livers were excised, rinsed, blotted and weighed. Sections were snap frozen in liquid nitrogen, fixed in 10% neutral buffered formalin or preserved in RNAlater (Qiagen). Mortality was less than 5% and not associated with a particular genoptype.
Sample_growth_protocol_ch1	Tob1 null mice on a C57BL/6 background were a generous gift from Dr. Indrani Bagchi (University of Ilinois at Urbana-Champaign). These mice are derived from a line produced and maintained by Dr. Tadashi Yamamoto. Animals were maintained in a pathogenfree facility administered by Harvard Medical School in accordance with the institutional guidelines of Harvard Medical School and Beth Israel Deaconess Medical Center.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	CEL files were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM) mismatch (MM) difference model.
Sample_platform_id	GPL1261
Sample_contact_name	Hasan,Huseyin,Otu
Sample_contact_email	hotu@bidmc.harvard.edu
Sample_contact_laboratory	BIDMC Genomics Center
Sample_contact_department	Medicine
Sample_contact_institute	Harvard Medical School
Sample_contact_address	4 Blackfan Circle Rm. 238
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM543nnn/GSM543430/suppl/GSM543430.CEL.gz
Sample_series_id	GSE21836
Sample_data_row_count	45101

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