Search results for the GEO ID: GSE21900 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM544741 | GPL1261 |
|
P1-control-ex1
|
P1 retinas of control mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P1
|
Gene expression data from the control retina at P1
|
Sample_geo_accession | GSM544741
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544741/suppl/GSM544741_P1-ex1-WT.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544742 | GPL1261 |
|
P1-control-ex2
|
P1 retinas of control mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P1
|
Gene expression data from the control retina at P1
|
Sample_geo_accession | GSM544742
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544742/suppl/GSM544742_P1-ex2-WT.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544743 | GPL1261 |
|
P1-control-ex3
|
P1 retinas of control mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P1
|
Gene expression data from the Otx2 CKO retina at P1
|
Sample_geo_accession | GSM544743
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544743/suppl/GSM544743_P1-ex3-WT.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544744 | GPL1261 |
|
P1-Otx2-CKO-ex1
|
P1 retinas of Otx2 CKO mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre+
time: P1
|
Gene expression data from the control retina at P1
|
Sample_geo_accession | GSM544744
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544744/suppl/GSM544744_P1-ex1-CKO.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544745 | GPL1261 |
|
P1-Otx2-CKO-ex2
|
P1 retinas of Otx2 CKO mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre+
time: P1
|
Gene expression data from the control retina at P1
|
Sample_geo_accession | GSM544745
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544745/suppl/GSM544745_P1-ex2-CKO.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544746 | GPL1261 |
|
P1-Otx2-CKO-ex3
|
P1 retinas of Otx2 CKO mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre+
time: P1
|
Gene expression data from the control retina at P1
|
Sample_geo_accession | GSM544746
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544746/suppl/GSM544746_P1-ex3-CKO.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544747 | GPL1261 |
|
P12-control-ex1
|
P12 retinas of control mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P12
|
Gene expression data from the control retina at P12
|
Sample_geo_accession | GSM544747
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544747/suppl/GSM544747_P12_ex1_WT.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544748 | GPL1261 |
|
P12-control-ex2
|
P12 retinas of control mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P12
|
Gene expression data from the control retina at P12
|
Sample_geo_accession | GSM544748
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544748/suppl/GSM544748_P12-ex2-WT.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544749 | GPL1261 |
|
P12-control-ex3
|
P12 retinas of control mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P12
|
Gene expression data from the Otx2 CKO retina at P12
|
Sample_geo_accession | GSM544749
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544749/suppl/GSM544749_P12-ex3-WT.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544750 | GPL1261 |
|
P12-Otx2-CKO-ex1
|
P12 retinas of Otx2 CKO mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre+
time: P12
|
Gene expression data from the control retina at P12
|
Sample_geo_accession | GSM544750
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544750/suppl/GSM544750_P12-ex1-CKO.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544751 | GPL1261 |
|
P12-Otx2-CKO-ex2
|
P12 retinas of Otx2 CKO mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre+
time: P12
|
Gene expression data from the control retina at P12
|
Sample_geo_accession | GSM544751
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544751/suppl/GSM544751_P12-ex2-CKO.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
| |
|
GSM544752 | GPL1261 |
|
P12-Otx2-CKO-ex3
|
P12 retinas of Otx2 CKO mice
|
tissue: retina
genotype: otx2 flox/flox; Crx-Cre+
time: P12
|
Gene expression data from the control retina at P12
|
Sample_geo_accession | GSM544752
| Sample_status | Public on May 18 2011
| Sample_submission_date | May 19 2010
| Sample_last_update_date | May 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The mouse retinas were dissected using forceps.
| Sample_extract_protocol_ch1 | Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared using IVT labeling kit.
| Sample_hyb_protocol | cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000-7G.
| Sample_data_processing | Signal intensity was determined using GeneChip Operating Software 1.4.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahisa,,Furukawa
| Sample_contact_laboratory | Furukawa Lab
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Osaka Bioscience Institute
| Sample_contact_address | 6-2-4 Furuedai
| Sample_contact_city | Suita
| Sample_contact_state | Osaka
| Sample_contact_zip/postal_code | 565-0874
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM544nnn/GSM544752/suppl/GSM544752_P12-ex3-CKO.CEL.gz
| Sample_series_id | GSE21900
| Sample_data_row_count | 45101
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
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