Search results for the GEO ID: GSE21912  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM545145 | GPL570 |
|
Ctl-shRNA 24 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 24 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545145
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545145/suppl/GSM545145.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545146 | GPL570 |
|
Ctl-shRNA 24 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 24 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545146
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545146/suppl/GSM545146.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545147 | GPL570 |
|
Ctl-shRNA 24 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 24 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545147
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545147/suppl/GSM545147.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545148 | GPL570 |
|
Ctl-shRNA + dox 24 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 24 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545148
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545148/suppl/GSM545148.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545149 | GPL570 |
|
Ctl-shRNA + dox 24 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 24 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545149
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545149/suppl/GSM545149.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545150 | GPL570 |
|
Ctl-shRNA + dox 24 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 24 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545150
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545150/suppl/GSM545150.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545151 | GPL570 |
|
Bmi1-shRNA 24 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 24 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545151
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545151/suppl/GSM545151.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545152 | GPL570 |
|
Bmi1-shRNA 24 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 24 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545152
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545152/suppl/GSM545152.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545153 | GPL570 |
|
Bmi1-shRNA 24 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 24 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545153
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545153/suppl/GSM545153.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545154 | GPL570 |
|
Bmi1-shRNA + dox 24 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 24 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545154
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545154/suppl/GSM545154.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545155 | GPL570 |
|
Bmi1-shRNA + dox 24 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 24 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545155
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545155/suppl/GSM545155.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545156 | GPL570 |
|
Bmi1-shRNA + dox 24 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 24 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545156
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545156/suppl/GSM545156.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545157 | GPL570 |
|
Ctl-shRNA 32 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 32 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545157
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545157/suppl/GSM545157.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545158 | GPL570 |
|
Ctl-shRNA 32 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 32 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545158
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545158/suppl/GSM545158.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545159 | GPL570 |
|
Ctl-shRNA 32 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 32 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545159
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545159/suppl/GSM545159.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545160 | GPL570 |
|
Ctl-shRNA + dox 32 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 32 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545160
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545160/suppl/GSM545160.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545161 | GPL570 |
|
Ctl-shRNA + dox 32 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 32 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545161
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545161/suppl/GSM545161.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545162 | GPL570 |
|
Ctl-shRNA + dox 32 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 32 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545162
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545162/suppl/GSM545162.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545163 | GPL570 |
|
Bmi1-shRNA 32 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 32 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545163
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545163/suppl/GSM545163.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545164 | GPL570 |
|
Bmi1-shRNA 32 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 32 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545164
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545164/suppl/GSM545164.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545165 | GPL570 |
|
Bmi1-shRNA 32 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 32 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545165
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545165/suppl/GSM545165.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545166 | GPL570 |
|
Bmi1-shRNA + dox 32 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 32 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545166
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545166/suppl/GSM545166.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545167 | GPL570 |
|
Bmi1-shRNA + dox 32 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 32 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545167
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545167/suppl/GSM545167.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545168 | GPL570 |
|
Bmi1-shRNA + dox 32 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 32 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545168
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545168/suppl/GSM545168.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545169 | GPL570 |
|
Ctl-shRNA 48 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 48 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545169
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545169/suppl/GSM545169.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545170 | GPL570 |
|
Ctl-shRNA 48 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 48 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545170
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545170/suppl/GSM545170.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545171 | GPL570 |
|
Ctl-shRNA 48 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 48 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545171
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545171/suppl/GSM545171.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545172 | GPL570 |
|
Ctl-shRNA + dox 48 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 48 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545172
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545172/suppl/GSM545172.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545173 | GPL570 |
|
Ctl-shRNA + dox 48 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 48 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545173
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545173/suppl/GSM545173.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545174 | GPL570 |
|
Ctl-shRNA + dox 48 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: dox
time post-induction: 48 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545174
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545174/suppl/GSM545174.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545175 | GPL570 |
|
Bmi1-shRNA 48 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 48 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545175
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545175/suppl/GSM545175.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545176 | GPL570 |
|
Bmi1-shRNA 48 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 48 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545176
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545176/suppl/GSM545176.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545177 | GPL570 |
|
Bmi1-shRNA 48 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: no dox
time post-induction: 48 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545177
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545177/suppl/GSM545177.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545178 | GPL570 |
|
Bmi1-shRNA + dox 48 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 48 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545178
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545178/suppl/GSM545178.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545179 | GPL570 |
|
Bmi1-shRNA + dox 48 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 48 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545179
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545179/suppl/GSM545179.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545180 | GPL570 |
|
Bmi1-shRNA + dox 48 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 48 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible BMI1 shRNA
|
| Sample_geo_accession | GSM545180
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545180/suppl/GSM545180.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545181 | GPL570 |
|
Ctl-shRNA 0 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 0 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545181
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545181/suppl/GSM545181.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545182 | GPL570 |
|
Ctl-shRNA 0 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 0 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545182
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545182/suppl/GSM545182.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545183 | GPL570 |
|
Ctl-shRNA 0 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: control
dox induction: no dox
time post-induction: 0 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545183
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545183/suppl/GSM545183.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545184 | GPL570 |
|
Bmi1-shRNA 0 hrs (1)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 0 hours
replicate: 1
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545184
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545184/suppl/GSM545184.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545185 | GPL570 |
|
Bmi1-shRNA 0 hrs (2)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 0 hours
replicate: 2
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545185
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545185/suppl/GSM545185.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
| | |
|
GSM545186 | GPL570 |
|
Bmi1-shRNA 0 hrs (3)
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 0 hours
replicate: 3
|
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Sample_geo_accession | GSM545186
| | Sample_status | Public on May 19 2010
| | Sample_submission_date | May 19 2010
| | Sample_last_update_date | May 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | If dox induction, then shRNAs induced using 100ng/ml doxycline.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
| | Sample_hyb_protocol | Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Morrissey
| | Sample_contact_email | michael.morrissey@novartis.com
| | Sample_contact_phone | 617-871-7418
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | 250 Massachusetts Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM545nnn/GSM545186/suppl/GSM545186.cel.gz
| | Sample_series_id | GSE21912
| | Sample_data_row_count | 54675
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