Search results for the GEO ID: GSE21989  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM546908 | GPL570 |
|
HUVEC at 0 min., control, biological replicate 1
|
HUVEC at 0 min., non exposed to insulin, biological replicate 1
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 0 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546908
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546908/suppl/GSM546908.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546909 | GPL570 |
|
HUVEC at 0 min., control, biological replicate 2
|
HUVEC at 0 min., non exposed to insulin, biological replicate 2
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 0 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546909
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546909/suppl/GSM546909.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546910 | GPL570 |
|
HUVEC at 40 min., control
|
HUVEC at 40 min., non exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 40 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546910
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546910/suppl/GSM546910.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546911 | GPL570 |
|
HUVEC at 100 min., control
|
HUVEC at 100 min., non exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 100 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546911
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546911/suppl/GSM546911.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546912 | GPL570 |
|
HUVEC at 200 min., control
|
HUVEC at 200 min., non exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 200 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546912
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546912/suppl/GSM546912.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546913 | GPL570 |
|
HUVEC at 340 min., control
|
HUVEC at 340 min., non exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 340 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546913
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546913/suppl/GSM546913.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546914 | GPL570 |
|
HUVEC at 440 min., control
|
HUVEC at 440 min., non exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: none
time point: 440 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546914
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546914/suppl/GSM546914.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546915 | GPL570 |
|
HUVEC at 40 min., exposed to insulin
|
HUVEC at 40 min., exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: insulin
time point: 40 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546915
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546915/suppl/GSM546915.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546916 | GPL570 |
|
HUVEC at 100 min., exposed to insulin
|
HUVEC at 100 min., exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: insulin
time point: 100 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546916
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546916/suppl/GSM546916.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546917 | GPL570 |
|
HUVEC at 200 min., exposed to insulin
|
HUVEC at 200 min., exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: insulin
time point: 200 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546917
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546917/suppl/GSM546917.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
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GSM546918 | GPL570 |
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HUVEC at 340 min., exposed to insulin
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HUVEC at 340 min., exposed to insulin
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cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: insulin
time point: 340 min
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Gene expression data from HUVEC
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| Sample_geo_accession | GSM546918
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546918/suppl/GSM546918.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
| | |
|
GSM546919 | GPL570 |
|
HUVEC at 440 min., exposed to insulin
|
HUVEC at 440 min., exposed to insulin
|
cell type: Normal Human Umbilical Vein Endothelial Cells (HUVEC)
agent: insulin
time point: 440 min
|
Gene expression data from HUVEC
|
| Sample_geo_accession | GSM546919
| | Sample_status | Public on May 26 2010
| | Sample_submission_date | May 25 2010
| | Sample_last_update_date | May 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were seeded onto six well plates at a density of 2X105 per well; 1 ml of quiescent medium, in absence or presence of 1 mU/ml (7 nM) insulin (Calbiochem-Inalco Spa), was added to each well.
| | Sample_growth_protocol_ch1 | Cells were cultured in Endothelial Cell Growth Medium (Promocell), supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, Saint Louis, USA), 0.02% Supplement Mix/ Endothelial Cell Growth Medium (PromoCell), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using commercially available kit (TRIzol Reagent, Invitrogen) and stored at -80°C. The samples were further purified using RNeasy mini kit (Qiagen, Milan, Italy) following manufacturer's recommendations. The quality and quantity of total RNA was measured using the Agilent test on a Bioanalyzer (Agilent Technologies, PAlo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Sample labeling was performed using protocolos described in the Affymetrix GeneChip expression analysis tecnical manual.
| | Sample_hyb_protocol | Hybridization were performed using protocols described in the Affymetrix GeneChip expression analysis technical manual.
| | Sample_scan_protocol | Image quantification was performed using GeneChip (Affymetrix, SantaClara, CA) scanner and software.
| | Sample_data_processing | Preprocessing steps such as background substraction, probe cell normalization and expression level calculations, were performed using quantile normalization and Robust Microarray Analysis (RMA) software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tiziana,,Sanavia
| | Sample_contact_email | sanaviat@dei.unipd.it
| | Sample_contact_institute | University of Padova
| | Sample_contact_address | Gradenigo, 6/B
| | Sample_contact_city | Padova
| | Sample_contact_zip/postal_code | 35131
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM546nnn/GSM546919/suppl/GSM546919.CEL.gz
| | Sample_series_id | GSE21989
| | Sample_data_row_count | 54675
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