Search results for the GEO ID: GSE22025 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM547227 | GPL570 |
|
[CD4+ T-Cells Cord Blood]_[Control]_[1]
|
Human Cord Blood
|
tissue type: Cord Blood
cell type: CD4+ T cells
treatment: absence of progesterone + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547227
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547227/suppl/GSM547227_003467_H_4.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547227/suppl/GSM547227_003467_H_4.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547228 | GPL570 |
|
[CD4+ T-Cells Cord Blood]_[Progesterone]_[1]
|
Human Cord Blood
|
tissue type: Cord Blood
cell type: CD4+ T cells
treatment: presence of progesterone + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547228
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547228/suppl/GSM547228_003468_H_4P.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547228/suppl/GSM547228_003468_H_4P.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547229 | GPL570 |
|
[CD4+ T-Cells Cord Blood]_[TGFb]_[1]
|
Human Cord Blood
|
tissue type: Cord Blood
cell type: CD4+ T cells
treatment: presence of TGFb1 + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547229
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547229/suppl/GSM547229_003469_H_4T.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547229/suppl/GSM547229_003469_H_4T.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547230 | GPL570 |
|
[CD4+ T-Cells Cord Blood]_[Progesterone+TGFb]_[1]
|
Human Cord Blood
|
tissue type: Cord Blood
cell type: CD4+ T cells
treatment: presence of progesterone + TGFb1 + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547230
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547230/suppl/GSM547230_003470_H_4PT.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547230/suppl/GSM547230_003470_H_4PT.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547231 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[Control]_[1]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: absence of progesterone + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547231
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547231/suppl/GSM547231_003471_H_4_PB.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547231/suppl/GSM547231_003471_H_4_PB.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547232 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[Progesterone]_[1]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: presence of progesterone + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547232
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547232/suppl/GSM547232_003472_H_4P_PB.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547232/suppl/GSM547232_003472_H_4P_PB.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547233 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[TGFb]_[1]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: presence of TGFb1 + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547233
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547233/suppl/GSM547233_003473_H_4T_PB.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547233/suppl/GSM547233_003473_H_4T_PB.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547234 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[Progesterone+TGFb]_[1]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: presence of progesterone + TGFb1 + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547234
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA Isolation Kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547234/suppl/GSM547234_003474_H_4PT_PB.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547234/suppl/GSM547234_003474_H_4PT_PB.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547235 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[Control]_[2]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: absence of progesterone + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay.
|
Sample_geo_accession | GSM547235
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_data_processing |
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547235/suppl/GSM547235_003475_H_4_PB3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547235/suppl/GSM547235_003475_H_4_PB3.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547236 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[Progesterone]_[2]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: presence of progesterone + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay.
|
Sample_geo_accession | GSM547236
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_data_processing |
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547236/suppl/GSM547236_003476_H_4P_PB3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547236/suppl/GSM547236_003476_H_4P_PB3.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
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GSM547237 | GPL570 |
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[CD4+ T-Cells Peripheral Blood]_[Progesterone+TGFb_[2]
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Human Peripheral Blood
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tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: presence of progesterone + TGFb1 + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay.
|
Sample_geo_accession | GSM547237
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_data_processing |
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547237/suppl/GSM547237_003478_H_4PT_PB3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547237/suppl/GSM547237_003478_H_4PT_PB3.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
GSM547254 | GPL570 |
|
[CD4+ T-Cells Peripheral Blood]_[TGFb]_[2]
|
Human Peripheral Blood
|
tissue type: Peripheral Blood
cell type: CD4+ T cells
treatment: presence of TGFb1 + IL-2
|
Human CD4 T cells were cultured in vitro to obtain RNA for the assay
|
Sample_geo_accession | GSM547254
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | May 26 2010
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_extract_protocol_ch1 | Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Human HG_U133_Plus_2 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled for High-Resolution Scanning. The instruments were controlled using GCOS version 1.4 software.
| Sample_data_processing | The data were processed with MAS 5.0 using the GeneChip Operation Software.
| Sample_data_processing |
| Sample_platform_id | GPL570
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547254/suppl/GSM547254_003477_H_4T_PB3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM547nnn/GSM547254/suppl/GSM547254_003477_H_4T_PB3.CHP.gz
| Sample_series_id | GSE22025
| Sample_data_row_count | 54675
| |
|
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