Result

Search results for the GEO ID: GSE22046

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM548002

GPL1261

trained wild type mouse gastrocnemius, biological rep1

Gastrocnemius (Medial & Lateral), trained, 6 months

strain: Mixed SV/129 & C57BL/6 sex: Female tissue: Gastrocnemius (Medial & Lateral) genotype: Wild type age: 8 months training: 6 months of training

Myogenin gene deletion performed at 3 months of age

Sample_geo_accession	GSM548002
Sample_status	Public on May 29 2010
Sample_submission_date	May 28 2010
Sample_last_update_date	May 28 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Tamoxifen-induced myogenin gene deletion performed at 2-3 months of age. Unrestricted access to exercise running wheels between 2-3 months of age to 8-9 months of age. Involuntary treadmill running for 2 weeks following training.
Sample_growth_protocol_ch1	Mice were allowed unrestricted access to voluntary exercise running wheels for 6 months and then subjected to an involuntary treadmill for 2 weeks to determine overall exercise endurance.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the small-sample Affymetrix protocol from 100 ng total RNA (Eukaryotic Small Sample Target Labeling Technique Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChiP Scanner 3000, 7G Upgrade
Sample_data_processing	The data were analyzed with Genespring version 11.0.1 (RMA algorithm). The baseline transformation was set to the median of all samples. Unpaired two-tailed t-tests were performed and significance cutoff was set to p<0.05. Fold-change cutoff was set to FC>=1.4
Sample_platform_id	GPL1261
Sample_contact_name	JAMES,ERIC,MEADOWS
Sample_contact_email	ERMEADOWS@MDANDERSON.ORG
Sample_contact_phone	713-834-6264
Sample_contact_laboratory	KLEIN
Sample_contact_department	BIOCHEMISTRY
Sample_contact_institute	MD ANDERSON CANCER CENTER
Sample_contact_address	1515 HOLCOMBE BLVD
Sample_contact_city	HOUSTON
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548002/suppl/GSM548002_949_WK_001_07-03-09.CEL.gz
Sample_series_id	GSE22046
Sample_data_row_count	45101

GSM548003

GPL1261

trained wild type mouse gastrocnemius, biological rep2

Gastrocnemius (Medial & Lateral), trained, 6 months

strain: Mixed SV/129 & C57BL/6 sex: Female tissue: Gastrocnemius (Medial & Lateral) genotype: Wild type age: 8 months training: 6 months of training

Myogenin gene deletion performed at 3 months of age

Sample_geo_accession	GSM548003
Sample_status	Public on May 29 2010
Sample_submission_date	May 28 2010
Sample_last_update_date	May 28 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Tamoxifen-induced myogenin gene deletion performed at 2-3 months of age. Unrestricted access to exercise running wheels between 2-3 months of age to 8-9 months of age. Involuntary treadmill running for 2 weeks following training.
Sample_growth_protocol_ch1	Mice were allowed unrestricted access to voluntary exercise running wheels for 6 months and then subjected to an involuntary treadmill for 2 weeks to determine overall exercise endurance.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the small-sample Affymetrix protocol from 100 ng total RNA (Eukaryotic Small Sample Target Labeling Technique Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChiP Scanner 3000, 7G Upgrade
Sample_data_processing	The data were analyzed with Genespring version 11.0.1 (RMA algorithm). The baseline transformation was set to the median of all samples. Unpaired two-tailed t-tests were performed and significance cutoff was set to p<0.05. Fold-change cutoff was set to FC>=1.4
Sample_platform_id	GPL1261
Sample_contact_name	JAMES,ERIC,MEADOWS
Sample_contact_email	ERMEADOWS@MDANDERSON.ORG
Sample_contact_phone	713-834-6264
Sample_contact_laboratory	KLEIN
Sample_contact_department	BIOCHEMISTRY
Sample_contact_institute	MD ANDERSON CANCER CENTER
Sample_contact_address	1515 HOLCOMBE BLVD
Sample_contact_city	HOUSTON
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548003/suppl/GSM548003_949_WK_005_07-03-09.CEL.gz
Sample_series_id	GSE22046
Sample_data_row_count	45101

GSM548004

GPL1261

trained wild type mouse gastrocnemius, biological rep3

Gastrocnemius (Medial & Lateral), trained, 6 months

strain: Mixed SV/129 & C57BL/6 sex: Female tissue: Gastrocnemius (Medial & Lateral) genotype: Wild type age: 8 months training: 6 months of training

Myogenin gene deletion performed at 3 months of age

Sample_geo_accession	GSM548004
Sample_status	Public on May 29 2010
Sample_submission_date	May 28 2010
Sample_last_update_date	May 28 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Tamoxifen-induced myogenin gene deletion performed at 2-3 months of age. Unrestricted access to exercise running wheels between 2-3 months of age to 8-9 months of age. Involuntary treadmill running for 2 weeks following training.
Sample_growth_protocol_ch1	Mice were allowed unrestricted access to voluntary exercise running wheels for 6 months and then subjected to an involuntary treadmill for 2 weeks to determine overall exercise endurance.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the small-sample Affymetrix protocol from 100 ng total RNA (Eukaryotic Small Sample Target Labeling Technique Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChiP Scanner 3000, 7G Upgrade
Sample_data_processing	The data were analyzed with Genespring version 11.0.1 (RMA algorithm). The baseline transformation was set to the median of all samples. Unpaired two-tailed t-tests were performed and significance cutoff was set to p<0.05. Fold-change cutoff was set to FC>=1.4
Sample_platform_id	GPL1261
Sample_contact_name	JAMES,ERIC,MEADOWS
Sample_contact_email	ERMEADOWS@MDANDERSON.ORG
Sample_contact_phone	713-834-6264
Sample_contact_laboratory	KLEIN
Sample_contact_department	BIOCHEMISTRY
Sample_contact_institute	MD ANDERSON CANCER CENTER
Sample_contact_address	1515 HOLCOMBE BLVD
Sample_contact_city	HOUSTON
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548004/suppl/GSM548004_949_WK_006_07-03-09.CEL.gz
Sample_series_id	GSE22046
Sample_data_row_count	45101

GSM548005

GPL1261

trained myog-deleted mouse gastrocnemius, biological rep1

Gastrocnemius (Medial & Lateral), trained, 6 months

strain: Mixed SV/129 & C57BL/6 sex: Female tissue: Gastrocnemius (Medial & Lateral) genotype: Myog-deleted age: 8 months training: 6 months of training

Myogenin gene deletion performed at 3 months of age

Sample_geo_accession	GSM548005
Sample_status	Public on May 29 2010
Sample_submission_date	May 28 2010
Sample_last_update_date	May 28 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Tamoxifen-induced myogenin gene deletion performed at 2-3 months of age. Unrestricted access to exercise running wheels between 2-3 months of age to 8-9 months of age. Involuntary treadmill running for 2 weeks following training.
Sample_growth_protocol_ch1	Mice were allowed unrestricted access to voluntary exercise running wheels for 6 months and then subjected to an involuntary treadmill for 2 weeks to determine overall exercise endurance.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the small-sample Affymetrix protocol from 100 ng total RNA (Eukaryotic Small Sample Target Labeling Technique Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChiP Scanner 3000, 7G Upgrade
Sample_data_processing	The data were analyzed with Genespring version 11.0.1 (RMA algorithm). The baseline transformation was set to the median of all samples. Unpaired two-tailed t-tests were performed and significance cutoff was set to p<0.05. Fold-change cutoff was set to FC>=1.4
Sample_platform_id	GPL1261
Sample_contact_name	JAMES,ERIC,MEADOWS
Sample_contact_email	ERMEADOWS@MDANDERSON.ORG
Sample_contact_phone	713-834-6264
Sample_contact_laboratory	KLEIN
Sample_contact_department	BIOCHEMISTRY
Sample_contact_institute	MD ANDERSON CANCER CENTER
Sample_contact_address	1515 HOLCOMBE BLVD
Sample_contact_city	HOUSTON
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548005/suppl/GSM548005_949_WK_002_07-03-09.CEL.gz
Sample_series_id	GSE22046
Sample_data_row_count	45101

GSM548006

GPL1261

trained myog-deleted mouse gastrocnemius, biological rep2

Gastrocnemius (Medial & Lateral), trained, 6 months

strain: Mixed SV/129 & C57BL/6 sex: Female tissue: Gastrocnemius (Medial & Lateral) genotype: Myog-deleted age: 8 months training: 6 months of training

Myogenin gene deletion performed at 3 months of age

Sample_geo_accession	GSM548006
Sample_status	Public on May 29 2010
Sample_submission_date	May 28 2010
Sample_last_update_date	May 28 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Tamoxifen-induced myogenin gene deletion performed at 2-3 months of age. Unrestricted access to exercise running wheels between 2-3 months of age to 8-9 months of age. Involuntary treadmill running for 2 weeks following training.
Sample_growth_protocol_ch1	Mice were allowed unrestricted access to voluntary exercise running wheels for 6 months and then subjected to an involuntary treadmill for 2 weeks to determine overall exercise endurance.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the small-sample Affymetrix protocol from 100 ng total RNA (Eukaryotic Small Sample Target Labeling Technique Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChiP Scanner 3000, 7G Upgrade
Sample_data_processing	The data were analyzed with Genespring version 11.0.1 (RMA algorithm). The baseline transformation was set to the median of all samples. Unpaired two-tailed t-tests were performed and significance cutoff was set to p<0.05. Fold-change cutoff was set to FC>=1.4
Sample_platform_id	GPL1261
Sample_contact_name	JAMES,ERIC,MEADOWS
Sample_contact_email	ERMEADOWS@MDANDERSON.ORG
Sample_contact_phone	713-834-6264
Sample_contact_laboratory	KLEIN
Sample_contact_department	BIOCHEMISTRY
Sample_contact_institute	MD ANDERSON CANCER CENTER
Sample_contact_address	1515 HOLCOMBE BLVD
Sample_contact_city	HOUSTON
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548006/suppl/GSM548006_949_WK_003_07-03-09.CEL.gz
Sample_series_id	GSE22046
Sample_data_row_count	45101

GSM548007

GPL1261

trained myog-deleted mouse gastrocnemius, biological rep3

Gastrocnemius (Medial & Lateral), trained, 6 months

strain: Mixed SV/129 & C57BL/6 sex: Female tissue: Gastrocnemius (Medial & Lateral) genotype: Myog-deleted age: 8 months training: 6 months of training

Myogenin gene deletion performed at 3 months of age

Sample_geo_accession	GSM548007
Sample_status	Public on May 29 2010
Sample_submission_date	May 28 2010
Sample_last_update_date	May 28 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Tamoxifen-induced myogenin gene deletion performed at 2-3 months of age. Unrestricted access to exercise running wheels between 2-3 months of age to 8-9 months of age. Involuntary treadmill running for 2 weeks following training.
Sample_growth_protocol_ch1	Mice were allowed unrestricted access to voluntary exercise running wheels for 6 months and then subjected to an involuntary treadmill for 2 weeks to determine overall exercise endurance.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the small-sample Affymetrix protocol from 100 ng total RNA (Eukaryotic Small Sample Target Labeling Technique Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChiP Scanner 3000, 7G Upgrade
Sample_data_processing	The data were analyzed with Genespring version 11.0.1 (RMA algorithm). The baseline transformation was set to the median of all samples. Unpaired two-tailed t-tests were performed and significance cutoff was set to p<0.05. Fold-change cutoff was set to FC>=1.4
Sample_platform_id	GPL1261
Sample_contact_name	JAMES,ERIC,MEADOWS
Sample_contact_email	ERMEADOWS@MDANDERSON.ORG
Sample_contact_phone	713-834-6264
Sample_contact_laboratory	KLEIN
Sample_contact_department	BIOCHEMISTRY
Sample_contact_institute	MD ANDERSON CANCER CENTER
Sample_contact_address	1515 HOLCOMBE BLVD
Sample_contact_city	HOUSTON
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548007/suppl/GSM548007_949_WK_004_07-03-09.CEL.gz
Sample_series_id	GSE22046
Sample_data_row_count	45101

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