Result

Search results for the GEO ID: GSE22081

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Title

Source name

Description

Characteristics

GSM548962

GPL1261

STAT4-wild type T cells, cultured 7 days in Th1 condition

primary CD4+ T cells from spleen and lymph nodes

cell: CD4+ T cell genotype: C57BL/6J (wild type)

Gene expression data from wild type CD4+ T cells cultured for 7 days in Th1 condition

Sample_geo_accession	GSM548962
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 01 2010
Sample_last_update_date	Jun 18 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 7 day culture in vitro, cells were restimulated for 2hr with plate-bound anti-CD3, anti-CD28 (10 mg each) and IL-12 (20 ng/ml) for Th1 condition or IL-4 (10 ng/ml) for Th2 condition.
Sample_growth_protocol_ch1	Control and STAT-deficient naïve CD4+CD44-CD62L+ T cells were isolated and sorted on the flow cytometer. Cells were cultured for 72h with platebound anti-CD3, anti-CD28 (10 mg/ml each), IL-12 (20 ng/ml) and anti-IL-4 (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-12 (10 ng/ml) for 4 days (Th1 conditions), or anti-CD3, anti-CD28 (10 mg/ml each), IL-4 (10 ng/ml) and anti-IFN-g (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-4 (10 ng/ml) for 4 days (Th2 conditions).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the MirVana RNA Isolation kit from Ambion.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was biotin-labeled using the MessageAmpII-Biotin Enhanced Kit from Ambion
Sample_hyb_protocol	aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
Sample_scan_protocol	Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software.
Sample_data_processing	Data processing were done using Affymetrix Power Tool (APT) v. 1.10.2, quantile normalization RMA
Sample_platform_id	GPL1261
Sample_contact_name	Golnaz,,Vahedi
Sample_contact_laboratory	Lymphocyte Cell Biology Section
Sample_contact_department	NIAMS
Sample_contact_institute	National Institutes of Health
Sample_contact_address	9000 Rockville Pike Bldg 10 Rm 13C101A
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548962/suppl/GSM548962.CEL.gz
Sample_series_id	GSE22081
Sample_series_id	GSE22105
Sample_data_row_count	45101

GSM548963

GPL1261

STAT4-deficient T cells, cultured 7 days in Th1 condition

primary CD4+ T cells from spleen and lymph nodes

cell: CD4+ T cell genotype: C57BL6-STAT4 (STAT4KO)

Gene expression data from STAT4-deficient CD4+ T cells cultured for 7 days in Th1 condition

Sample_geo_accession	GSM548963
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 01 2010
Sample_last_update_date	Jun 18 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 7 day culture in vitro, cells were restimulated for 2hr with plate-bound anti-CD3, anti-CD28 (10 mg each) and IL-12 (20 ng/ml) for Th1 condition or IL-4 (10 ng/ml) for Th2 condition.
Sample_growth_protocol_ch1	Control and STAT-deficient naïve CD4+CD44-CD62L+ T cells were isolated and sorted on the flow cytometer. Cells were cultured for 72h with platebound anti-CD3, anti-CD28 (10 mg/ml each), IL-12 (20 ng/ml) and anti-IL-4 (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-12 (10 ng/ml) for 4 days (Th1 conditions), or anti-CD3, anti-CD28 (10 mg/ml each), IL-4 (10 ng/ml) and anti-IFN-g (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-4 (10 ng/ml) for 4 days (Th2 conditions).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the MirVana RNA Isolation kit from Ambion.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was biotin-labeled using the MessageAmpII-Biotin Enhanced Kit from Ambion
Sample_hyb_protocol	aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
Sample_scan_protocol	Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software.
Sample_data_processing	Data processing were done using Affymetrix Power Tool (APT) v. 1.10.2, quantile normalization RMA
Sample_platform_id	GPL1261
Sample_contact_name	Golnaz,,Vahedi
Sample_contact_laboratory	Lymphocyte Cell Biology Section
Sample_contact_department	NIAMS
Sample_contact_institute	National Institutes of Health
Sample_contact_address	9000 Rockville Pike Bldg 10 Rm 13C101A
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548963/suppl/GSM548963.CEL.gz
Sample_series_id	GSE22081
Sample_series_id	GSE22105
Sample_data_row_count	45101

GSM548964

GPL1261

STAT6-wild type T cells, cultured 7 days in Th2 condition

primary CD4+ T cells from spleen and lymph nodes

cell: CD4+ T cell genotype: C57BL/6J (wild type)

Gene expression data from wild type CD4+ T cells cultured for 7 days in Th2 condition

Sample_geo_accession	GSM548964
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 01 2010
Sample_last_update_date	Jun 18 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 7 day culture in vitro, cells were restimulated for 2hr with plate-bound anti-CD3, anti-CD28 (10 mg each) and IL-12 (20 ng/ml) for Th1 condition or IL-4 (10 ng/ml) for Th2 condition.
Sample_growth_protocol_ch1	Control and STAT-deficient naïve CD4+CD44-CD62L+ T cells were isolated and sorted on the flow cytometer. Cells were cultured for 72h with platebound anti-CD3, anti-CD28 (10 mg/ml each), IL-12 (20 ng/ml) and anti-IL-4 (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-12 (10 ng/ml) for 4 days (Th1 conditions), or anti-CD3, anti-CD28 (10 mg/ml each), IL-4 (10 ng/ml) and anti-IFN-g (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-4 (10 ng/ml) for 4 days (Th2 conditions).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the MirVana RNA Isolation kit from Ambion.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was biotin-labeled using the MessageAmpII-Biotin Enhanced Kit from Ambion
Sample_hyb_protocol	aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
Sample_scan_protocol	Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software.
Sample_data_processing	Data processing were done using Affymetrix Power Tool (APT) v. 1.10.2, quantile normalization RMA
Sample_platform_id	GPL1261
Sample_contact_name	Golnaz,,Vahedi
Sample_contact_laboratory	Lymphocyte Cell Biology Section
Sample_contact_department	NIAMS
Sample_contact_institute	National Institutes of Health
Sample_contact_address	9000 Rockville Pike Bldg 10 Rm 13C101A
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548964/suppl/GSM548964.CEL.gz
Sample_series_id	GSE22081
Sample_series_id	GSE22105
Sample_data_row_count	45101

GSM548965

GPL1261

STAT6-deficient T cells, cultured 7 days in Th2 condition

primary CD4+ T cells from spleen and lymph nodes

Gene expression data from STAT6-deficient CD4+ T cells cultured for 7 days in Th2 condition

Sample_geo_accession	GSM548965
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 01 2010
Sample_last_update_date	Jun 18 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 7 day culture in vitro, cells were restimulated for 2hr with plate-bound anti-CD3, anti-CD28 (10 mg each) and IL-12 (20 ng/ml) for Th1 condition or IL-4 (10 ng/ml) for Th2 condition.
Sample_growth_protocol_ch1	Control and STAT-deficient naïve CD4+CD44-CD62L+ T cells were isolated and sorted on the flow cytometer. Cells were cultured for 72h with platebound anti-CD3, anti-CD28 (10 mg/ml each), IL-12 (20 ng/ml) and anti-IL-4 (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-12 (10 ng/ml) for 4 days (Th1 conditions), or anti-CD3, anti-CD28 (10 mg/ml each), IL-4 (10 ng/ml) and anti-IFN-g (10 mg/ml) for 3 days, followed by IL-2 (50 U/ml) and IL-4 (10 ng/ml) for 4 days (Th2 conditions).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the MirVana RNA Isolation kit from Ambion.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was biotin-labeled using the MessageAmpII-Biotin Enhanced Kit from Ambion
Sample_hyb_protocol	aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
Sample_scan_protocol	Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software.
Sample_data_processing	Data processing were done using Affymetrix Power Tool (APT) v. 1.10.2, quantile normalization RMA
Sample_platform_id	GPL1261
Sample_contact_name	Golnaz,,Vahedi
Sample_contact_laboratory	Lymphocyte Cell Biology Section
Sample_contact_department	NIAMS
Sample_contact_institute	National Institutes of Health
Sample_contact_address	9000 Rockville Pike Bldg 10 Rm 13C101A
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM548nnn/GSM548965/suppl/GSM548965.CEL.gz
Sample_series_id	GSE22081
Sample_series_id	GSE22105
Sample_data_row_count	45101

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