Search results for the GEO ID: GSE22097  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM549530 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen1
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: male
age (years): 60
bmi (body mass index, kg/m2): 24
|
Gene expression data from surgical samples
Surgical-760
|
| Sample_geo_accession | GSM549530
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549530/suppl/GSM549530_Jos4209.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549531 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen2
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: male
age (years): 72
bmi (body mass index, kg/m2): 35
|
Gene expression data from surgical samples
Surgical-799
|
| Sample_geo_accession | GSM549531
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549531/suppl/GSM549531_Jos4211.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549532 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen3
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: male
age (years): 72
bmi (body mass index, kg/m2): 22
|
Gene expression data from surgical samples
Surgical-824
|
| Sample_geo_accession | GSM549532
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549532/suppl/GSM549532_Jos4323.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549533 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen4
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: female
age (years): 67
bmi (body mass index, kg/m2): 26
|
Gene expression data from surgical samples
Surgical-795
|
| Sample_geo_accession | GSM549533
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549533/suppl/GSM549533_Jos4327.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549534 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen5
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: male
age (years): 81
bmi (body mass index, kg/m2): 23
|
Gene expression data from surgical samples
Surgical-564
|
| Sample_geo_accession | GSM549534
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549534/suppl/GSM549534_Jos4347.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549535 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen6
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: female
age (years): 57
bmi (body mass index, kg/m2): 24
|
Gene expression data from surgical samples
Surgical-850
|
| Sample_geo_accession | GSM549535
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549535/suppl/GSM549535_Jos4348.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549536 | GPL1352 |
|
beta-cells_non-diabetic condition_surgical specimen7
|
human beta-cells_non-diabetic
|
tissue: pancreatic islets
cell type: beta-cell
protocol: non-diabetic condition
gender: female
age (years): 77
bmi (body mass index, kg/m2): 26
|
Gene expression data from surgical samples
Surgical-861
|
| Sample_geo_accession | GSM549536
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549536/suppl/GSM549536_Jos4349.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549537 | GPL1352 |
|
transplanted beta-cells_hyperglycemic condition_donor1_sample_a
|
human beta-cells exposed to mild hyperglycemia in transplant site
|
tissue: pancreatic islets
cell type: beta-cell
protocol: hyperglycemic condition
gender: male
age (years): 35
bmi (body mass index, kg/m2): 30
|
Gene expression data from transplanted islets
111705-5RHuGraft
|
| Sample_geo_accession | GSM549537
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549537/suppl/GSM549537_jos3622.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549538 | GPL1352 |
|
transplanted beta-cells_hyperglycemic condition_donor1_sample_b
|
human beta-cells exposed to mild hyperglycemia in transplant site
|
tissue: pancreatic islets
cell type: beta-cell
protocol: hyperglycemic condition
gender: male
age (years): 35
bmi (body mass index, kg/m2): 30
|
Gene expression data from transplanted islets
111705-5LHuGraft
|
| Sample_geo_accession | GSM549538
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549538/suppl/GSM549538_jos3623.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549539 | GPL1352 |
|
transplanted beta-cells_hyperglycemic condition_donor1_sample_c
|
human beta-cells exposed to mild hyperglycemia in transplant site
|
tissue: pancreatic islets
cell type: beta-cell
protocol: hyperglycemic condition
gender: male
age (years): 35
bmi (body mass index, kg/m2): 30
|
Gene expression data from transplanted islets
111705-5NHuGraft
|
| Sample_geo_accession | GSM549539
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549539/suppl/GSM549539_jos3624.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549540 | GPL1352 |
|
transplanted beta-cells_hyperglycemic condition_donor2_sample_a
|
human beta-cells exposed to mild hyperglycemia in transplant site
|
tissue: pancreatic islets
cell type: beta-cell
protocol: hyperglycemic condition
gender: male
age (years): 57
bmi (body mass index, kg/m2): 28
|
Gene expression data from transplanted islets
1M-NT060606-4L
|
| Sample_geo_accession | GSM549540
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549540/suppl/GSM549540_jos4004.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549541 | GPL1352 |
|
transplanted beta-cells_hyperglycemic condition_donor2_sample_b
|
human beta-cells exposed to mild hyperglycemia in transplant site
|
tissue: pancreatic islets
cell type: beta-cell
protocol: hyperglycemic condition
gender: male
age (years): 57
bmi (body mass index, kg/m2): 28
|
Gene expression data from transplanted islets
1M-NT060606-4B
|
| Sample_geo_accession | GSM549541
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549541/suppl/GSM549541_jos4005.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
GSM549542 | GPL1352 |
|
transplanted beta-cells_hyperglycemic condition_donor2_sample_c
|
human beta-cells exposed to mild hyperglycemia in transplant site
|
tissue: pancreatic islets
cell type: beta-cell
protocol: hyperglycemic condition
gender: male
age (years): 57
bmi (body mass index, kg/m2): 28
|
Gene expression data from transplanted islets
1M-NT060606-5R
|
| Sample_geo_accession | GSM549542
| | Sample_status | Public on Jun 02 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pancreas specimens obtained from surgical samples and islet grafts recovered from the transplanted mice were placed in cryomolds, embedded in Tissue-Tek OCT medium, immediately frozen in chilled isopentane, and stored at –80°C, pending sectioning at 8 μm. Frozen pancreatic sections were fixed in 70% ethanol for 30 seconds, rinsed in diethylpyrocarbonate (DEPC)-treated water for 30 sec, and dehydrated in 70% ethanol for 30 sec, 100% ethanol (rinsing), 100% ethanol twice for 1 min, and xylene for 4 minutes. Sections were air-dried and laser capture microdissection was performed. The microdissected cells were incubated with 10 μl of guanidine thiocyanate and polyethylene glycol octylphenol ether-based buffer for 30 minutes at 42°C, and the samples stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each population of laser captured β–cells using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers. RNA amplification was performed using RiboAmp HS RNA Amplification Kit (Arcturus) following the manufacture’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was converted into double-stranded complementary DNA (cDNA) using the RiboAmp HS RNA Amplification Kit (Arcturus), and biotinylated complementary RNA (cRNA) was generated from cDNA by in vitro transcription reaction using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics). RNA products were purified using the MiraColTM Purification Columns (Arcturus).
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented to nucleotide stretches of 30-200 nucleotides and hybridized for 16hr at 45C to the Affymetrix Human X3P array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using DNA-chip Analyzer software (dChip), using the invariant set normalization algorithm.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Lorella,,Marselli
| | Sample_contact_email | Lorella.Marselli@med.unipi.it
| | Sample_contact_phone | +39 050 995135
| | Sample_contact_fax | +39 050 541521
| | Sample_contact_laboratory | Section on Islet Transplantation and Cell Biology
| | Sample_contact_department | Joslin Diabetes Center and the Department of Medicine
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | One Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549542/suppl/GSM549542_jos4006.CEL.gz
| | Sample_series_id | GSE22097
| | Sample_data_row_count | 61295
| | |
|
| |
| |
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