Search results for the GEO ID: GSE22103  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM549573 | GPL570 |
|
Unst_1
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, Unstimulated
|
| Sample_geo_accession | GSM549573
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549573/suppl/GSM549573_S_250854_Plus_R1.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549574 | GPL570 |
|
Unst_2
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, Unstimulated
|
| Sample_geo_accession | GSM549574
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549574/suppl/GSM549574_S_250855_Plus_R1.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549575 | GPL570 |
|
Unst_3
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, Unstimulated
|
| Sample_geo_accession | GSM549575
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549575/suppl/GSM549575_S_250856_Plus_R1.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549576 | GPL570 |
|
Unst_4
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, Unstimulated
|
| Sample_geo_accession | GSM549576
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549576/suppl/GSM549576_S_250857_Plus_R1.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549577 | GPL570 |
|
LPS_1
|
Microfluidics isolated human neutrophils, LPS stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: LPS stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, LPS stimulated
|
| Sample_geo_accession | GSM549577
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549577/suppl/GSM549577_S_250858_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549578 | GPL570 |
|
LPS_2
|
Microfluidics isolated human neutrophils, LPS stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: LPS stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, LPS stimulated
|
| Sample_geo_accession | GSM549578
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549578/suppl/GSM549578_S_250859_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549579 | GPL570 |
|
LPS_3
|
Microfluidics isolated human neutrophils, LPS stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: LPS stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, LPS stimulated
|
| Sample_geo_accession | GSM549579
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549579/suppl/GSM549579_S_250860_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549580 | GPL570 |
|
LPS_4
|
Microfluidics isolated human neutrophils, LPS stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: LPS stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, LPS stimulated
|
| Sample_geo_accession | GSM549580
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549580/suppl/GSM549580_S_250861_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549581 | GPL570 |
|
GM-CSF_IFNr_1
|
Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: GM-CSF and IFNg stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549581
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549581/suppl/GSM549581_S_250862_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549582 | GPL570 |
|
GM-CSF_IFNr_2
|
Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: GM-CSF and IFNg stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549582
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549582/suppl/GSM549582_S_250863_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549583 | GPL570 |
|
GM-CSF_IFNr_3
|
Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: GM-CSF and IFNg stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549583
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549583/suppl/GSM549583_S_250864_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549584 | GPL570 |
|
GM-CSF_IFNr_4
|
Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: GM-CSF and IFNg stimulated
subject: 0
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549584
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549584/suppl/GSM549584_S_250865_Plus_R1.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549585 | GPL570 |
|
UD-G-02
|
Density centrifugation with Ficoll-dextran human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Density centrifugation with Ficoll-dextran
condition: Unstimulated
subject: 1
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549585
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549585/suppl/GSM549585_4729.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549586 | GPL570 |
|
UC-G-02
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 1
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549586
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549586/suppl/GSM549586_4731.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549587 | GPL570 |
|
UD-G-03
|
Density centrifugation with Ficoll-dextran human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Density centrifugation with Ficoll-dextran
condition: Unstimulated
subject: 2
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549587
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549587/suppl/GSM549587_4735.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549588 | GPL570 |
|
UC-G-03
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 2
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549588
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549588/suppl/GSM549588_4737.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549589 | GPL570 |
|
UD-G-04
|
Density centrifugation with Ficoll-dextran human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Density centrifugation with Ficoll-dextran
condition: Unstimulated
subject: 3
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549589
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549589/suppl/GSM549589_4741.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549590 | GPL570 |
|
UC-G-04
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 3
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549590
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549590/suppl/GSM549590_4743.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549591 | GPL570 |
|
UD-G-05
|
Density centrifugation with Ficoll-dextran human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Density centrifugation with Ficoll-dextran
condition: Unstimulated
subject: 4
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549591
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549591/suppl/GSM549591_4747.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549592 | GPL570 |
|
UC-G-05
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 4
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549592
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549592/suppl/GSM549592_4749.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549593 | GPL570 |
|
UD-G-06
|
Density centrifugation with Ficoll-dextran human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Density centrifugation with Ficoll-dextran
condition: Unstimulated
subject: 5
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549593
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549593/suppl/GSM549593_4753.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
GSM549594 | GPL570 |
|
UC-G-06
|
Microfluidics isolated human neutrophils, Unstimulated
|
cell type: human neutrophils
isolation: Microfluidics isolated
condition: Unstimulated
subject: 5
|
Gene expression data from Microfluidics isolated human neutrophils, GM-CSF and IFNg stimulated
|
| Sample_geo_accession | GSM549594
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Jun 02 2010
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stimulated cells were treated with LPS (50 ng/ml) or GM-CSF (20 ng/ml) + IFN-g(100 IU/ml) (abbreviated as GM+I) for 16 hours on a rocker at 37 °C in 5% CO2 atmosphere before cell isolation.
| | Sample_growth_protocol_ch1 | Freshly drawn peripheral blood was anti-coagulated with sodium heparin and was directly processed for cell isolation by microfluidics (designated as unstimulated) or stimulated before cell isolation. In addition to microfluidic processing, leukocytes were isolated using density gradient techniques from both unstimulated and stimulated whole blood.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction followed a modified commercial protocol (QIAGEN RNeasy Plus). To remove genomic DNA, 350 l of guanadinium isothiocyanate with detergent (Qiagen RLT Plus Buffer) was added to the thawed, original lysate along with 2 mercaptoethaol (Sigma) at 20 l/ml. This combined lysate was then processed according to manufacturer protocols (Qiagen RNeasy Plus Kit) yielding purified total RNA analyzed on an Agilent Bioanalyzer 2100 system.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies) from 20 ng of total RNA as starting material.
| | Sample_hyb_protocol | the labeled cDNA was hybridized onto GeneChip™ Human Genome U133 Plus 2.0 Arrays according to standard Affymetrix protocol
| | Sample_scan_protocol | Chips were washed and scanned as recommended by the Ovation System User Guide (version 1.0).
| | Sample_data_processing | Low level analysis was performed using dChip using the perfect match only option.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenzhong,,xiao
| | Sample_contact_email | WXIAO1@PARTNERS.ORG
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 55 Fruit Street, GRB 1302
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114-2696
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM549nnn/GSM549594/suppl/GSM549594_4755.CEL.gz
| | Sample_series_id | GSE22103
| | Sample_data_row_count | 54675
| | |
|
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