Search results for the GEO ID: GSE22117  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM550109 | GPL1261 |
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Control brain
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MRTFA-/-;MRTF-BFlox/Flox mouse brain at P0
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tissue: brain cortex
genotype: MRTFA-/-;MRTF-BFlox/Flox
age: P0
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Gene expression data from MRTFA-/-;MRTF-BFlox/Flox mouse brain at P0
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| Sample_geo_accession | GSM550109
| | Sample_status | Public on Jun 04 2010
| | Sample_submission_date | Jun 03 2010
| | Sample_last_update_date | Jun 03 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cortical brain tissues were extracted from three control MRTFA-/-;MRTF-BFlox/Flox and three MRTF-A-/-;MRTF-BF/F;GFAP-Cre mice at postnatal day 0. Extracts were pooled in control and double knockout groups.
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mayssa,H.,Mokalled
| | Sample_contact_laboratory | Eric Olson
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | UT Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 Harry Hines Blvd.
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9148
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550109/suppl/GSM550109.CEL.gz
| | Sample_series_id | GSE22117
| | Sample_data_row_count | 45101
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GSM550110 | GPL1261 |
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MRTFA-B double knockout brain
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MRTF-A-/-;MRTF-BF/F;GFAP-Cre mouse brain at P0
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tissue: brain cortex
genotype: MRTF-A-/-;MRTF-BF/F;GFAP-Cre
age: P0
|
Gene expression data from MRTF-A-/-;MRTF-BF/F;GFAP-Cre mouse brain at P1
|
| Sample_geo_accession | GSM550110
| | Sample_status | Public on Jun 04 2010
| | Sample_submission_date | Jun 03 2010
| | Sample_last_update_date | Jun 03 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cortical brain tissues were extracted from three control MRTFA-/-;MRTF-BFlox/Flox and three MRTF-A-/-;MRTF-BF/F;GFAP-Cre mice at postnatal day 0. Extracts were pooled in control and double knockout groups.
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mayssa,H.,Mokalled
| | Sample_contact_laboratory | Eric Olson
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | UT Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 Harry Hines Blvd.
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9148
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550110/suppl/GSM550110.CEL.gz
| | Sample_series_id | GSE22117
| | Sample_data_row_count | 45101
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