Result

Search results for the GEO ID: GSE22118

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Title

Source name

Description

Characteristics

GSM550111

GPL1355

FRTL-5, TSHR-WT, no_iodine, replicate 1

Thyroid cell line

iodine treatment: none chloride treatment: 1mM stable human tsh receptor expression: WT

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550111
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550111/suppl/GSM550111.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550111/suppl/GSM550111.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550112

GPL1355

FRTL-5, TSHR-WT, no_iodine, replicate 2

Thyroid cell line

iodine treatment: none chloride treatment: 1mM stable human tsh receptor expression: WT

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550112
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550112/suppl/GSM550112.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550112/suppl/GSM550112.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550113

GPL1355

FRTL-5, TSHR-WT, iodine, replicate 1

Thyroid cell line

iodine treatment: 1mM chloride treatment: none stable human tsh receptor expression: WT

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550113
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550113/suppl/GSM550113.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550113/suppl/GSM550113.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550114

GPL1355

FRTL-5, TSHR-WT, iodine, replicate 2

Thyroid cell line

iodine treatment: 1mM chloride treatment: none stable human tsh receptor expression: WT

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550114
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550114/suppl/GSM550114.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550114/suppl/GSM550114.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550115

GPL1355

FRTL-5, TSHR-L629F, no_iodine, replicate 1

Thyroid cell line

iodine treatment: none chloride treatment: 1mM stable human tsh receptor expression: L629F

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550115
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550115/suppl/GSM550115.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550115/suppl/GSM550115.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550116

GPL1355

FRTL-5, TSHR-L629F, no_iodine, replicate 2

Thyroid cell line

iodine treatment: none chloride treatment: 1mM stable human tsh receptor expression: L629F

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550116
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550116/suppl/GSM550116.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550116/suppl/GSM550116.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550117

GPL1355

FRTL-5, TSHR-L629F, iodine, replicate 1

Thyroid cell line

iodine treatment: 1mM chloride treatment: none stable human tsh receptor expression: L629F

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550117
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550117/suppl/GSM550117.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550117/suppl/GSM550117.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

GSM550118

GPL1355

FRTL-5, TSHR-L629F, iodine, replicate 2

Thyroid cell line

iodine treatment: 1mM chloride treatment: none stable human tsh receptor expression: L629F

SB5 sub-clone of FRTL-5

Sample_geo_accession	GSM550118
Sample_status	Public on Dec 01 2010
Sample_submission_date	Jun 03 2010
Sample_last_update_date	Aug 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cells were treated with 1mM sodium iodide or 1mM sodium chloride as an isotonic control.
Sample_growth_protocol_ch1	FRTL-5 cells were cultured under standard conditions with 5 mU/ml bovine TSH.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_label_protocol_ch1	staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining.
Sample_hyb_protocol	Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
Sample_platform_id	GPL1355
Sample_contact_name	Knut,,Krohn
Sample_contact_laboratory	DNA Technologies
Sample_contact_department	IZKF
Sample_contact_institute	University Leipzig
Sample_contact_address	Inselstraße 22
Sample_contact_city	Leipzig
Sample_contact_zip/postal_code	04103
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550118/suppl/GSM550118.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550118/suppl/GSM550118.CHP.gz
Sample_series_id	GSE22118
Sample_data_row_count	31099

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