Search results for the GEO ID: GSE22152  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM550942 | GPL570 |
|
GC resistant clone C7H2-R10E7 treated for 6 hours with EtOH
|
GC resistant clone C7H2-R10E7 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: EtOH
|
GC resistant clone C7H2-R10E7 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550942
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550942/suppl/GSM550942.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550943 | GPL570 |
|
GC resistant clone C7H2-R19E5 treated for 6 hours with EtOH
|
GC resistant clone C7H2-R19E5 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: EtOH
|
GC resistant clone C7H2-R19E5 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550943
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550943/suppl/GSM550943.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550944 | GPL570 |
|
GC resistant clone C7H2-R9C10 treated for 6 hours with EtOH
|
GC resistant clone C7H2-R9C10 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: EtOH
|
GC resistant clone C7H2-R9C10 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550944
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550944/suppl/GSM550944.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550945 | GPL570 |
|
GC resistant clone C7H2-R3B5 treated for 6 hours with EtOH
|
GC resistant clone C7H2-R3B5 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: EtOH
|
GC resistant clone C7H2-R3B5 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550945
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550945/suppl/GSM550945.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550946 | GPL570 |
|
GC resistant clone C7H2-R19F2 treated for 6 hours with EtOH
|
GC resistant clone C7H2-R19F2 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: EtOH
|
GC resistant clone C7H2-R19F2 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550946
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550946/suppl/GSM550946.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550947 | GPL570 |
|
GC resistant clone C7H2-R19E7 treated for 6 hours with EtOH
|
GC resistant clone C7H2-R19E7 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: EtOH
|
GC resistant clone C7H2-R19E7 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550947
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550947/suppl/GSM550947.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550948 | GPL570 |
|
GC sensitive clone C7H2-S11 treated for 6 hours with EtOH
|
GC sensitive clone C7H2-S11 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: EtOH
|
GC sensitive clone C7H2-S11 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550948
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550948/suppl/GSM550948.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550949 | GPL570 |
|
GC sensitive clone C7H2-S12 treated for 6 hours with EtOH
|
GC sensitive clone C7H2-S12 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: EtOH
|
GC sensitive clone C7H2-S12 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550949
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550949/suppl/GSM550949.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550950 | GPL570 |
|
GC sensitive clone C7H2-S27(ZR) treated for 6 hours with EtOH
|
GC sensitive clone C7H2-S27(ZR) treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: EtOH
|
GC sensitive clone C7H2-S27(ZR) treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550950
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550950/suppl/GSM550950.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550951 | GPL570 |
|
GC sensitive clone C7H2-S10 treated for 6 hours with EtOH
|
GC sensitive clone C7H2-S10 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: EtOH
|
GC sensitive clone C7H2-S10 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550951
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550951/suppl/GSM550951.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550952 | GPL570 |
|
GC sensitive clone C7H2-S2 treated for 6 hours with EtOH
|
GC sensitive clone C7H2-S2 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: EtOH
|
GC sensitive clone C7H2-S2 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550952
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550952/suppl/GSM550952.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550953 | GPL570 |
|
GC sensitive clone C7H2-S15 treated for 6 hours with EtOH
|
GC sensitive clone C7H2-S15 treated for 6 hours with EtOH
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: EtOH
|
GC sensitive clone C7H2-S15 treated for 6 hours with 0.1% ethanol (carrier control).
|
| Sample_geo_accession | GSM550953
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550953/suppl/GSM550953.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550954 | GPL570 |
|
GC resistant clone C7H2-R10E7 treated for 6 hours with GC
|
GC resistant clone C7H2-R10E7 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: GC
|
GC resistant clone C7H2-R10E7 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550954
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550954/suppl/GSM550954.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550955 | GPL570 |
|
GC resistant clone C7H2-R19E5 treated for 6 hours with GC
|
GC resistant clone C7H2-R19E5 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: GC
|
GC resistant clone C7H2-R19E5 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550955
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550955/suppl/GSM550955.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550956 | GPL570 |
|
GC resistant clone C7H2-R9C10 treated for 6 hours with GC
|
GC resistant clone C7H2-R9C10 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: GC
|
GC resistant clone C7H2-R9C10 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550956
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550956/suppl/GSM550956.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550957 | GPL570 |
|
GC resistant clone C7H2-R3B5 treated for 6 hours with GC
|
GC resistant clone C7H2-R3B5 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: GC
|
GC resistant clone C7H2-R3B5 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550957
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550957/suppl/GSM550957.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550958 | GPL570 |
|
GC resistant clone C7H2-R19F2 treated for 6 hours with GC
|
GC resistant clone C7H2-R19F2 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: GC
|
GC resistant clone C7H2-R19F2 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550958
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550958/suppl/GSM550958.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550959 | GPL570 |
|
GC resistant clone C7H2-R19E7 treated for 6 hours with GC
|
GC resistant clone C7H2-R19E7 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: TRUE
treatment: GC
|
GC resistant clone C7H2-R19E7 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550959
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550959/suppl/GSM550959.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550960 | GPL570 |
|
GC sensitive clone C7H2-S11 treated for 6 hours with GC
|
GC sensitive clone C7H2-S11 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: GC
|
GC sensitive clone C7H2-S11 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550960
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550960/suppl/GSM550960.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550961 | GPL570 |
|
GC sensitive clone C7H2-S12 treated for 6 hours with GC
|
GC sensitive clone C7H2-S12 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: GC
|
GC sensitive clone C7H2-S12 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550961
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550961/suppl/GSM550961.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550962 | GPL570 |
|
GC sensitive clone C7H2-S27 treated for 6 hours with GC
|
GC sensitive clone C7H2-S27 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: GC
|
GC sensitive clone C7H2-S27 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550962
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550962/suppl/GSM550962.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550963 | GPL570 |
|
GC sensitive clone C7H2-S10 treated for 6 hours with GC
|
GC sensitive clone C7H2-S10 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: GC
|
GC sensitive clone C7H2-S10 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550963
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550963/suppl/GSM550963.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550964 | GPL570 |
|
GC sensitive clone C7H2-S2 treated for 6 hours with GC
|
GC sensitive clone C7H2-S2 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: GC
|
GC sensitive clone C7H2-S2 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550964
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550964/suppl/GSM550964.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
|
GSM550965 | GPL570 |
|
GC sensitive clone C7H2-S15 treated for 6 hours with GC
|
GC sensitive clone C7H2-S15 treated for 6 hours with GC
|
cell type: lymphoblastic leukemia
gc resistant: FALSE
treatment: GC
|
GC sensitive clone C7H2-S15 treated for 6 hours with 100nM dexamethasone (a glucocorticoid).
|
| Sample_geo_accession | GSM550965
| | Sample_status | Public on Feb 01 2011
| | Sample_submission_date | Jun 04 2010
| | Sample_last_update_date | Feb 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100nM dexametasone (Sigma, Vienna, Austria) or 0.1% ethanol as carrier control.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal calf serum and 2mM L-glutamine at 37C, 5% carbon-dioxide and saturated humidity. The cells were free of mycoplamsa infection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
| | Sample_hyb_protocol | 20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix HG U133 Plus 2.0 GeneChips.
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | The raw data was preprocessed in R (version 2.10) using the GCRMA algorithm (Bioconductor's gcrma package).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM550nnn/GSM550965/suppl/GSM550965.CEL.gz
| | Sample_series_id | GSE22152
| | Sample_data_row_count | 54675
| | |
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