Search results for the GEO ID: GSE22167 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM551186 | GPL570 |
|
dH1F_Fibro_1
|
human fibroblast
|
cell type: fibroblast
cell line: GM04981 from Coriell
|
Gene expression data obtained from fibroblasts
|
Sample_geo_accession | GSM551186
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Coriell GM04981 http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM04981
| Sample_growth_protocol_ch1 | Fibroblasts were grown in DMEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551186/suppl/GSM551186.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551187 | GPL570 |
|
dH1F_Fibro_2
|
human fibroblast
|
cell type: fibroblast
cell line: GM04981 from Coriell
|
Gene expression data obtained from fibroblasts
|
Sample_geo_accession | GSM551187
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Coriell GM04981 http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM04981
| Sample_growth_protocol_ch1 | Fibroblasts were grown in DMEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551187/suppl/GSM551187_dH1F_Fibro_2.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551188 | GPL570 |
|
PB_CD34+_1
|
human peripheral blood derived CD34+ cells
|
cell type: peripheral blood derived CD34+ cells (PB_CD34+)
|
Gene expression data obtained from PB CD34+ blood
|
Sample_geo_accession | GSM551188
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal peripheral blood CD34+ (PB CD34+) and mononuclear cells (PBMCs) were either maintained in medium described previously (Loh et al. 2009) or grown in dendritic cell medium supplemented with SCF and GM-CSF
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551188/suppl/GSM551188.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551189 | GPL570 |
|
PB_CD34+_2
|
human peripheral blood derived CD34+ cells
|
cell type: peripheral blood derived CD34+ cells (PB_CD34+)
|
Gene expression data obtained from PB CD34+ blood
|
Sample_geo_accession | GSM551189
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal peripheral blood CD34+ (PB CD34+) and mononuclear cells (PBMCs) were either maintained in medium described previously (Loh et al. 2009) or grown in dendritic cell medium supplemented with SCF and GM-CSF
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551189/suppl/GSM551189_PB_CD34+_2.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551190 | GPL570 |
|
PBMCs_1
|
human peripheral blood derived mononuclear cells (PBMCs)
|
cell type: peripheral blood derived mononuclear cells (PBMCs)
|
Gene expression data obtained from PBMCs blood
|
Sample_geo_accession | GSM551190
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal peripheral blood CD34+ (PB CD34+) and mononuclear cells (PBMCs) were either maintained in medium described previously (Loh et al. 2009) or grown in dendritic cell medium supplemented with SCF and GM-CSF
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551190/suppl/GSM551190_PBMCs_1.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551191 | GPL570 |
|
PBMCs_2
|
human peripheral blood derived mononuclear cells (PBMCs)
|
cell type: peripheral blood derived mononuclear cells (PBMCs)
|
Gene expression data obtained from PBMCs blood
|
Sample_geo_accession | GSM551191
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal peripheral blood CD34+ (PB CD34+) and mononuclear cells (PBMCs) were either maintained in medium described previously (Loh et al. 2009) or grown in dendritic cell medium supplemented with SCF and GM-CSF
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551191/suppl/GSM551191.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551192 | GPL570 |
|
dH1F_iPS_1
|
iPS
|
cell type: iPS cells
iPS derived from: dH1F fibroblast
|
Gene expression data obtained from fibroblasts iPS
|
Sample_geo_accession | GSM551192
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551192/suppl/GSM551192.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551193 | GPL570 |
|
dH1F_iPS_2
|
iPS
|
cell type: iPS cells
iPS derived from: dH1F fibroblast
|
Gene expression data obtained from fibroblasts iPS
|
Sample_geo_accession | GSM551193
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551193/suppl/GSM551193.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551194 | GPL570 |
|
PB34_iPS1_1
|
iPS
|
cell type: iPS cells
iPS derived from: PB CD34+ blood
|
Gene expression data obtained from PB CD34+ blood iPS
|
Sample_geo_accession | GSM551194
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551194/suppl/GSM551194_PB34_iPS1_1.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551195 | GPL570 |
|
PB34_iPS1_2
|
iPS
|
cell type: iPS cells
iPS derived from: PB CD34+ blood
|
Gene expression data obtained from PB CD34+ blood iPS
|
Sample_geo_accession | GSM551195
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551195/suppl/GSM551195.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551196 | GPL570 |
|
PB34_iPS2_1
|
iPS
|
cell type: iPS cells
iPS derived from: PB CD34+ blood
|
Gene expression data obtained from PB CD34+ blood iPS
|
Sample_geo_accession | GSM551196
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551196/suppl/GSM551196.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551197 | GPL570 |
|
PB34_iPS2_2
|
iPS
|
cell type: iPS cells
iPS derived from: PB CD34+ blood
|
Gene expression data obtained from PB CD34+ blood iPS
|
Sample_geo_accession | GSM551197
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551197/suppl/GSM551197.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551198 | GPL570 |
|
PBMC(GH)_iPS1_1
|
iPS
|
cell type: iPS cells
iPS derived from: peripheral blood derived mononuclear cells (PBMCs)
|
Gene expression data obtained from PBMCs blood iPS
|
Sample_geo_accession | GSM551198
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551198/suppl/GSM551198.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551199 | GPL570 |
|
PBMC(GH)_iPS1_2
|
iPS
|
cell type: iPS cells
iPS derived from: peripheral blood derived mononuclear cells (PBMCs)
|
Gene expression data obtained from PBMCs blood iPS
|
Sample_geo_accession | GSM551199
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Jun 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551199/suppl/GSM551199.CEL.gz
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551200 | GPL570 |
|
H1_ES_1
|
human ES
|
cell type: ES
cell line: H1 Human embryonic stem cells
|
Gene expression data obtained from human ES
|
Sample_geo_accession | GSM551200
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551200/suppl/GSM551200.CEL.gz
| Sample_relation | Reanalyzed by: GSM579898
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551201 | GPL570 |
|
H1_ES_2
|
human ES
|
cell type: ES
cell line: H1 Human embryonic stem cells
|
Gene expression data obtained from human ES
|
Sample_geo_accession | GSM551201
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551201/suppl/GSM551201.CEL.gz
| Sample_relation | Reanalyzed by: GSM579899
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551202 | GPL570 |
|
H9_ES_1
|
human ES
|
cell type: ES
cell line: H9 Human embryonic stem cells
|
Gene expression data obtained from human ES
|
Sample_geo_accession | GSM551202
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551202/suppl/GSM551202.CEL.gz
| Sample_relation | Reanalyzed by: GSM579901
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
GSM551203 | GPL570 |
|
H9_ES_2
|
human ES
|
cell type: ES
cell line: H9 Human embryonic stem cells
|
Gene expression data obtained from human ES
|
Sample_geo_accession | GSM551203
| Sample_status | Public on Jun 10 2010
| Sample_submission_date | Jun 04 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| Sample_data_processing | RMA (Bioconductor Affy and RMA Packages).
| Sample_platform_id | GPL570
| Sample_contact_name | Hu,,Li
| Sample_contact_email | huli@bu.edu
| Sample_contact_laboratory | James Collins
| Sample_contact_institute | Wyss Institute
| Sample_contact_address | 3 Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM551nnn/GSM551203/suppl/GSM551203.CEL.gz
| Sample_relation | Reanalyzed by: GSM579902
| Sample_series_id | GSE22167
| Sample_data_row_count | 54675
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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