Result

Search results for the GEO ID: GSE22217

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM553172

GPL570

Polished_24h_rep1

Human osteoblasts cultured for 24 hours on titanium polished surfaces

cell type: osteoblast treatment: polished

Sample_geo_accession	GSM553172
Sample_status	Public on Jun 08 2011
Sample_submission_date	Jun 08 2010
Sample_last_update_date	Jun 08 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were cultured onto different titanium disks to test the effect of the surface modification. Titanium disks (n=6) were placed in a 6-well plate and 2x105 cells were seeded on each disk.
Sample_growth_protocol_ch1	Normal human osteoblasts (NHOst cell system, Cambrex BioScience, Walkersville, MD, USA) from tibia of one donor (male, forty-one years old) were grown in Osteoblast Growth Media (OGM) containing 10% fetal bovine serum, ascorbic acid and antibiotics (gentamicin sulfate and amphotetericin-B) (Cambrex BioScience, Walkersville, MD, USA). Cells were subcultured 1:4 before reaching confluence using PBS (PAA Laboratories GmbH, Austria) and trypsin/EDTA (Sigma, St. Louis, MO, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Trizol reagent (Life Technologies, Gaithersburg, MD, USA), according to manufacturer's instructions, and further purified using the RNeasy kit (Qiagen, Valencia, CA, USA) in order to remove organic components.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Double-stranded cDNA and biotin-labeled cRNA probes were created from 5 µg of total RNA using the Superscript Choice system (Invitrogen Life Technologies, Carlsbad, CA, USA) and the Bioarray (Enzo Biochem, New York, NY, USA), respectively.
Sample_hyb_protocol	Hybridization, washing and staining was performed on the GeneChips Fluidics Station 450 (Affymetrix), according to recommendations from Affymetrix
Sample_scan_protocol	The chips were scanned on the Affymetrix GeneArray 2500 scanner.
Sample_data_processing	The data sets were processed by the Affymetrix mas5.0 software, and signal values representing the expression level of each transcript were generated. Data were analyzed through the use of Ingenuity Pathways Analysis (Ingenuity® Systems, www.ingenuity.com)
Sample_platform_id	GPL570
Sample_contact_name	Marta,,Monjo
Sample_contact_email	marta.monjo@uib.es
Sample_contact_laboratory	Cell Therapy and Tissue Engineering Group
Sample_contact_department	IUNICS (Health Sciences Research Institute)
Sample_contact_institute	UIB (University of the Balearic Islands)
Sample_contact_address	Edifici del IUNICS, 1er pis, Laboratori de Biologia Molecular.Universitat de les Illes Balears. Cra. Valldemossa km 7.5
Sample_contact_city	Palma de Mallorca
Sample_contact_state	Illes Balears
Sample_contact_zip/postal_code	E-07122
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM553nnn/GSM553172/suppl/GSM553172.CEL.gz
Sample_series_id	GSE22217
Sample_data_row_count	54675

GSM553173

GPL570

Grit-Blasted_24h_rep1

Human osteoblasts cultured for 24 hours on titanium grit-blasted surfaces

cell type: osteoblast treatment: grit-blasted

Sample_geo_accession	GSM553173
Sample_status	Public on Jun 08 2011
Sample_submission_date	Jun 08 2010
Sample_last_update_date	Jun 08 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were cultured onto different titanium disks to test the effect of the surface modification. Titanium disks (n=6) were placed in a 6-well plate and 2x105 cells were seeded on each disk.
Sample_growth_protocol_ch1	Normal human osteoblasts (NHOst cell system, Cambrex BioScience, Walkersville, MD, USA) from tibia of one donor (male, forty-one years old) were grown in Osteoblast Growth Media (OGM) containing 10% fetal bovine serum, ascorbic acid and antibiotics (gentamicin sulfate and amphotetericin-B) (Cambrex BioScience, Walkersville, MD, USA). Cells were subcultured 1:4 before reaching confluence using PBS (PAA Laboratories GmbH, Austria) and trypsin/EDTA (Sigma, St. Louis, MO, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Trizol reagent (Life Technologies, Gaithersburg, MD, USA), according to manufacturer's instructions, and further purified using the RNeasy kit (Qiagen, Valencia, CA, USA) in order to remove organic components.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Double-stranded cDNA and biotin-labeled cRNA probes were created from 5 µg of total RNA using the Superscript Choice system (Invitrogen Life Technologies, Carlsbad, CA, USA) and the Bioarray (Enzo Biochem, New York, NY, USA), respectively.
Sample_hyb_protocol	Hybridization, washing and staining was performed on the GeneChips Fluidics Station 450 (Affymetrix), according to recommendations from Affymetrix
Sample_scan_protocol	The chips were scanned on the Affymetrix GeneArray 2500 scanner.
Sample_data_processing	The data sets were processed by the Affymetrix mas5.0 software, and signal values representing the expression level of each transcript were generated. Data were analyzed through the use of Ingenuity Pathways Analysis (Ingenuity® Systems, www.ingenuity.com)
Sample_platform_id	GPL570
Sample_contact_name	Marta,,Monjo
Sample_contact_email	marta.monjo@uib.es
Sample_contact_laboratory	Cell Therapy and Tissue Engineering Group
Sample_contact_department	IUNICS (Health Sciences Research Institute)
Sample_contact_institute	UIB (University of the Balearic Islands)
Sample_contact_address	Edifici del IUNICS, 1er pis, Laboratori de Biologia Molecular.Universitat de les Illes Balears. Cra. Valldemossa km 7.5
Sample_contact_city	Palma de Mallorca
Sample_contact_state	Illes Balears
Sample_contact_zip/postal_code	E-07122
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM553nnn/GSM553173/suppl/GSM553173.CEL.gz
Sample_series_id	GSE22217
Sample_data_row_count	54675

GSM553174

GPL570

Grit-Blasted-HFtreated_24h_rep1

Human osteoblasts cultured for 24 hours on titanium grit-blasted HF treated surfaces

cell type: osteoblast treatment: grit-blasted-HF treated

Sample_geo_accession	GSM553174
Sample_status	Public on Jun 08 2011
Sample_submission_date	Jun 08 2010
Sample_last_update_date	Jun 08 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were cultured onto different titanium disks to test the effect of the surface modification. Titanium disks (n=6) were placed in a 6-well plate and 2x105 cells were seeded on each disk.
Sample_growth_protocol_ch1	Normal human osteoblasts (NHOst cell system, Cambrex BioScience, Walkersville, MD, USA) from tibia of one donor (male, forty-one years old) were grown in Osteoblast Growth Media (OGM) containing 10% fetal bovine serum, ascorbic acid and antibiotics (gentamicin sulfate and amphotetericin-B) (Cambrex BioScience, Walkersville, MD, USA). Cells were subcultured 1:4 before reaching confluence using PBS (PAA Laboratories GmbH, Austria) and trypsin/EDTA (Sigma, St. Louis, MO, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Trizol reagent (Life Technologies, Gaithersburg, MD, USA), according to manufacturer's instructions, and further purified using the RNeasy kit (Qiagen, Valencia, CA, USA) in order to remove organic components.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Double-stranded cDNA and biotin-labeled cRNA probes were created from 5 µg of total RNA using the Superscript Choice system (Invitrogen Life Technologies, Carlsbad, CA, USA) and the Bioarray (Enzo Biochem, New York, NY, USA), respectively.
Sample_hyb_protocol	Hybridization, washing and staining was performed on the GeneChips Fluidics Station 450 (Affymetrix), according to recommendations from Affymetrix
Sample_scan_protocol	The chips were scanned on the Affymetrix GeneArray 2500 scanner.
Sample_data_processing	The data sets were processed by the Affymetrix mas5.0 software, and signal values representing the expression level of each transcript were generated. Data were analyzed through the use of Ingenuity Pathways Analysis (Ingenuity® Systems, www.ingenuity.com)
Sample_platform_id	GPL570
Sample_contact_name	Marta,,Monjo
Sample_contact_email	marta.monjo@uib.es
Sample_contact_laboratory	Cell Therapy and Tissue Engineering Group
Sample_contact_department	IUNICS (Health Sciences Research Institute)
Sample_contact_institute	UIB (University of the Balearic Islands)
Sample_contact_address	Edifici del IUNICS, 1er pis, Laboratori de Biologia Molecular.Universitat de les Illes Balears. Cra. Valldemossa km 7.5
Sample_contact_city	Palma de Mallorca
Sample_contact_state	Illes Balears
Sample_contact_zip/postal_code	E-07122
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM553nnn/GSM553174/suppl/GSM553174.CEL.gz
Sample_series_id	GSE22217
Sample_data_row_count	54675

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