Search results for the GEO ID: GSE22280  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM554392 | GPL1261 |
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KLS TIF1γ wtwt
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bone marrow KLS
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genotype: wild type
cell type: bone marrow lin-,c-kit+, Sca+ cell
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n/a
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| Sample_geo_accession | GSM554392
| | Sample_status | Public on Jun 25 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Jun 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | KLS cells purified from bone marrow of mice conditionally inactivated for TIF1γ gene and wildtype mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using RNeasy micro kit (qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ng total RNA (Expression Analysis Technical Manual:Two-cycle target labeling assay, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Affymetrix Microarray Suite version 5.0 (MAS 5.0) algorithms using global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Doulaye,,Dembele
| | Sample_contact_email | doulaye@igbmc.fr
| | Sample_contact_phone | +33 3 88 65 35 28
| | Sample_contact_department | Biopuces
| | Sample_contact_institute | IGBMC
| | Sample_contact_address | 1 rue Laurent Fries
| | Sample_contact_city | Illkirch
| | Sample_contact_zip/postal_code | 67400
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554392/suppl/GSM554392_PRO_wt-wt_430_2.CEL.gz
| | Sample_series_id | GSE22280
| | Sample_data_row_count | 45101
| | |
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GSM554393 | GPL1261 |
|
KLS TIF1γ Δ/Δ
|
bone marrow KLS
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genotype: TIF1γ Δ/Δ
cell type: bone marrow lin-,c-kit+, Sca+ cell
|
n/a
|
| Sample_geo_accession | GSM554393
| | Sample_status | Public on Jun 25 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Jun 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | KLS cells purified from bone marrow of mice conditionally inactivated for TIF1γ gene and wildtype mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using RNeasy micro kit (qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ng total RNA (Expression Analysis Technical Manual:Two-cycle target labeling assay, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Affymetrix Microarray Suite version 5.0 (MAS 5.0) algorithms using global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Doulaye,,Dembele
| | Sample_contact_email | doulaye@igbmc.fr
| | Sample_contact_phone | +33 3 88 65 35 28
| | Sample_contact_department | Biopuces
| | Sample_contact_institute | IGBMC
| | Sample_contact_address | 1 rue Laurent Fries
| | Sample_contact_city | Illkirch
| | Sample_contact_zip/postal_code | 67400
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554393/suppl/GSM554393_PRO_loxlox_430_2.CEL.gz
| | Sample_series_id | GSE22280
| | Sample_data_row_count | 45101
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