Search results for the GEO ID: GSE22293  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM554780 | GPL10526 |
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Control, 12h, rep3
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Coculture of 92% hMSCs and 8% huvEC, no treatment, after 12 hours
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cell type: co-culture of hMSC (92%) and hUVEC (8%)
treatment: control
|
The effect of Cyclopamine on expression of Hedgehog, angiogenesis and axon guidance marker genes was analyzed by seeding a coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days.
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| Sample_geo_accession | GSM554780
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days. Medium was changed every third day by transferring the spherical aggregate to a new plate.
| | Sample_growth_protocol_ch1 | Co-culture of hMSC (92%) and hUVEC (8%) were obtained by resuspending a total of 150,000 cell per spherical aggregate in 2 ml of differentiation medium and then seeded in a well of a Deepwell 96 well-plate (Nunc).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by using an RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | Affymetrix GeneArray Scanner3000
| | Sample_data_processing | The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of HG133 Plus2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
| | Sample_platform_id | GPL10526
| | Sample_contact_name | Carsten,,Sticht
| | Sample_contact_department | ZMF
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68169
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554780/suppl/GSM554780.CEL.gz
| | Sample_series_id | GSE22293
| | Sample_data_row_count | 17726
| | |
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GSM554781 | GPL10526 |
|
Control, 12h, rep4
|
Coculture of 92% hMSCs and 8% huvEC, no treatment, after 12 hours
|
cell type: co-culture of hMSC (92%) and hUVEC (8%)
treatment: control
|
The effect of Cyclopamine on expression of Hedgehog, angiogenesis and axon guidance marker genes was analyzed by seeding a coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days.
|
| Sample_geo_accession | GSM554781
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days. Medium was changed every third day by transferring the spherical aggregate to a new plate.
| | Sample_growth_protocol_ch1 | Co-culture of hMSC (92%) and hUVEC (8%) were obtained by resuspending a total of 150,000 cell per spherical aggregate in 2 ml of differentiation medium and then seeded in a well of a Deepwell 96 well-plate (Nunc).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by using an RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | Affymetrix GeneArray Scanner3000
| | Sample_data_processing | The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of HG133 Plus2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
| | Sample_platform_id | GPL10526
| | Sample_contact_name | Carsten,,Sticht
| | Sample_contact_department | ZMF
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68169
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554781/suppl/GSM554781.CEL.gz
| | Sample_series_id | GSE22293
| | Sample_data_row_count | 17726
| | |
|
GSM554782 | GPL10526 |
|
Cyclopamin, 12h, rep3
|
Coculture of 92% hMSCs and 8% huvEC, treated with cyclopamin, after 12 hours
|
cell type: co-culture of hMSC (92%) and hUVEC (8%)
treatment: Cyclopamine
|
The effect of Cyclopamine on expression of Hedgehog, angiogenesis and axon guidance marker genes was analyzed by seeding a coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days.
|
| Sample_geo_accession | GSM554782
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days. Medium was changed every third day by transferring the spherical aggregate to a new plate.
| | Sample_growth_protocol_ch1 | Co-culture of hMSC (92%) and hUVEC (8%) were obtained by resuspending a total of 150,000 cell per spherical aggregate in 2 ml of differentiation medium and then seeded in a well of a Deepwell 96 well-plate (Nunc).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by using an RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | Affymetrix GeneArray Scanner3000
| | Sample_data_processing | The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of HG133 Plus2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
| | Sample_platform_id | GPL10526
| | Sample_contact_name | Carsten,,Sticht
| | Sample_contact_department | ZMF
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68169
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554782/suppl/GSM554782.CEL.gz
| | Sample_series_id | GSE22293
| | Sample_data_row_count | 17726
| | |
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GSM554783 | GPL10526 |
|
Cyclopamin, 12h, rep2
|
Coculture of 92% hMSCs and 8% huvEC, treated with cyclopamin, after 12 hours
|
cell type: co-culture of hMSC (92%) and hUVEC (8%)
treatment: Cyclopamine
|
The effect of Cyclopamine on expression of Hedgehog, angiogenesis and axon guidance marker genes was analyzed by seeding a coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days.
|
| Sample_geo_accession | GSM554783
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days. Medium was changed every third day by transferring the spherical aggregate to a new plate.
| | Sample_growth_protocol_ch1 | Co-culture of hMSC (92%) and hUVEC (8%) were obtained by resuspending a total of 150,000 cell per spherical aggregate in 2 ml of differentiation medium and then seeded in a well of a Deepwell 96 well-plate (Nunc).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by using an RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | Affymetrix GeneArray Scanner3000
| | Sample_data_processing | The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of HG133 Plus2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
| | Sample_platform_id | GPL10526
| | Sample_contact_name | Carsten,,Sticht
| | Sample_contact_department | ZMF
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68169
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554783/suppl/GSM554783.CEL.gz
| | Sample_series_id | GSE22293
| | Sample_data_row_count | 17726
| | |
|
GSM554784 | GPL10526 |
|
Cyclopamin, 12h, rep1
|
Coculture of 92% hMSCs and 8% huvEC, treated with cyclopamin, after 12 hours
|
cell type: co-culture of hMSC (92%) and hUVEC (8%)
treatment: Cyclopamine
|
The effect of Cyclopamine on expression of Hedgehog, angiogenesis and axon guidance marker genes was analyzed by seeding a coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days.
|
| Sample_geo_accession | GSM554784
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days. Medium was changed every third day by transferring the spherical aggregate to a new plate.
| | Sample_growth_protocol_ch1 | Co-culture of hMSC (92%) and hUVEC (8%) were obtained by resuspending a total of 150,000 cell per spherical aggregate in 2 ml of differentiation medium and then seeded in a well of a Deepwell 96 well-plate (Nunc).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by using an RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | Affymetrix GeneArray Scanner3000
| | Sample_data_processing | The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of HG133 Plus2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
| | Sample_platform_id | GPL10526
| | Sample_contact_name | Carsten,,Sticht
| | Sample_contact_department | ZMF
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68169
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554784/suppl/GSM554784.CEL.gz
| | Sample_series_id | GSE22293
| | Sample_data_row_count | 17726
| | |
|
GSM554785 | GPL10526 |
|
Control, 12h, rep1
|
Coculture of 92% hMSCs and 8% huvEC, no treatment, after 12 hours
|
cell type: co-culture of hMSC (92%) and hUVEC (8%)
treatment: control
|
The effect of Cyclopamine on expression of Hedgehog, angiogenesis and axon guidance marker genes was analyzed by seeding a coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days.
|
| Sample_geo_accession | GSM554785
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Jun 10 2010
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | coculture of 92% hMSCs and 8% huvEC (150,000 cells in total) in Deepwell 96 well-plates (adapted hanging-drop method) supplemented or not in cyclopamine, for 12 days. Medium was changed every third day by transferring the spherical aggregate to a new plate.
| | Sample_growth_protocol_ch1 | Co-culture of hMSC (92%) and hUVEC (8%) were obtained by resuspending a total of 150,000 cell per spherical aggregate in 2 ml of differentiation medium and then seeded in a well of a Deepwell 96 well-plate (Nunc).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by using an RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | Affymetrix GeneArray Scanner3000
| | Sample_data_processing | The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of HG133 Plus2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
| | Sample_platform_id | GPL10526
| | Sample_contact_name | Carsten,,Sticht
| | Sample_contact_department | ZMF
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68169
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM554nnn/GSM554785/suppl/GSM554785.CEL.gz
| | Sample_series_id | GSE22293
| | Sample_data_row_count | 17726
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