Search results for the GEO ID: GSE22325  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM555847 | GPL570 |
|
HUVEC simulated 4h with % 0.01 BSA
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 4h with % 0.01 BSA
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555847
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555847/suppl/GSM555847.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555848 | GPL570 |
|
HUVEC simulated 8h with % 0.01 BSA
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 8h with % 0.01 BSA
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555848
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555848/suppl/GSM555848.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555849 | GPL570 |
|
HUVEC simulated 16h with % 0.01 BSA
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 16h with % 0.01 BSA
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555849
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555849/suppl/GSM555849.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555850 | GPL570 |
|
HUVEC simulated 48h with % 0.01 BSA
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 48h with % 0.01 BSA
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555850
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555850/suppl/GSM555850.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555851 | GPL570 |
|
HUVEC simulated 4h with IL-1beta(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 4h with IL-1beta(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555851
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555851/suppl/GSM555851.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555852 | GPL570 |
|
HUVEC simulated 8h with IL-1beta(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 8h with IL-1beta(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555852
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555852/suppl/GSM555852.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555853 | GPL570 |
|
HUVEC simulated 16h with IL-1beta(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 16h with IL-1beta(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555853
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555853/suppl/GSM555853.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555854 | GPL570 |
|
HUVEC simulated 4h with IL-3(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 4h with IL-3(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555854
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555854/suppl/GSM555854.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555855 | GPL570 |
|
HUVEC simulated 8h with IL-3(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 8h with IL-3(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555855
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555855/suppl/GSM555855.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555856 | GPL570 |
|
HUVEC simulated 16h with IL-3(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 16h with IL-3(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555856
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555856/suppl/GSM555856.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555857 | GPL570 |
|
HUVEC simulated 48h with IL-3(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 48h with IL-3(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555857
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555857/suppl/GSM555857.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555858 | GPL570 |
|
HUVEC simulated 4h with IL-6(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 4h with IL-6(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555858
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555858/suppl/GSM555858.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555859 | GPL570 |
|
HUVEC simulated 8h with IL-6(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 8h with IL-6(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555859
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555859/suppl/GSM555859.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555860 | GPL570 |
|
HUVEC simulated 16h with IL-6(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 16h with IL-6(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555860
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555860/suppl/GSM555860.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555861 | GPL570 |
|
HUVEC simulated 48h with IL-6(100 U)
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: simulated 48h with IL-6(100 U)
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555861
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555861/suppl/GSM555861.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555862 | GPL570 |
|
HUVEC non-stimulated, 0h biological rep 1
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: non-stimulated, 0h biological rep 1
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555862
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555862/suppl/GSM555862.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
| | |
|
GSM555863 | GPL570 |
|
HUVEC non-stimulated, 0h biological rep 2
|
Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: non-stimulated, 0h biological rep 2
|
Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
|
| Sample_geo_accession | GSM555863
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555863/suppl/GSM555863.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
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GSM555864 | GPL570 |
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HUVEC non-stimulated, 16h
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Human Umbilical Vein Endothelial Cells
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cell type: Human Umbilical Vein Endothelial Cells (HUVEC)
cell culture: Passage 3
pooling: pooled from 7 individual
treatment group: non-stimulated, 16h
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Gene expression data from interlekin stimulated HUVEC vs. BSA control stimulation in time course
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| Sample_geo_accession | GSM555864
| | Sample_status | Public on Jul 01 2011
| | Sample_submission_date | Jun 14 2010
| | Sample_last_update_date | Jul 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent monolayer ECs from the third passage were stimulated for either 4, 8 or 16 hours with 100 U/mL IL-1ß, or for 4, 8, 16 and 48 hours with 100 U/mL IL-3 or IL-6, and 0.01 % BSA. Medium only controls were also performed at 0 and 16 hours.
| | Sample_growth_protocol_ch1 | Cells were cultured in Earl´s M199 medium (Biochrom, Berlin, Germany) supplemented with 16% fetal bovine serum (FBS, Hyclone, South Logan, UT, USA), 4% human serum from healthy volunteers, 2 mM L-glutamine, 0.15 mg/mL endothelial growth factor supplement (Intracel; Rockville, MD, USA), 0.015 mg/mL heparin and 1% fungicide.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Following stimulation of HUVEC with the various interleukins, cells were harvested by collagenase treatment (0.1% in PBS). Total RNA was isolated from stimulated cells obtained at the various time points via a Qiagen RNeasy Mini Kit in accordance to the manufacturer’s protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
| | Sample_hyb_protocol | Fragmented cRNA (10 μg) were hybridized to HG-U133 plus 2.0 gene chips for 16 hours at 45°C.
| | Sample_scan_protocol | Following washing and staining, gene chips were scanned with the GeneArray scanner controlled by the Affymetrix GCOS 1.4 software.
| | Sample_data_processing | Raw data were processed and normalized by applying the GCOS 1.4 software according to Affymetrix recommendations and by the MAS5.0 normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gürkan,,Bal
| | Sample_contact_email | guerkan.bal@charite.de
| | Sample_contact_phone | +4930450553139
| | Sample_contact_fax | +49304507553139
| | Sample_contact_laboratory | Stem Cell Lab.
| | Sample_contact_department | Institute for Transfusion Medicine
| | Sample_contact_institute | Charité - Universitätsmedizin Berlin
| | Sample_contact_address | Augustenburger Platz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM555nnn/GSM555864/suppl/GSM555864.CEL.gz
| | Sample_series_id | GSE22325
| | Sample_data_row_count | 54675
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