Search results for the GEO ID: GSE22356  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM556413 | GPL570 |
|
SSc_102
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_102_1
|
| Sample_geo_accession | GSM556413
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556413/suppl/GSM556413.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556414 | GPL570 |
|
SSc_123
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_123_1
|
| Sample_geo_accession | GSM556414
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556414/suppl/GSM556414.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556415 | GPL570 |
|
SSc_126
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_126_4
|
| Sample_geo_accession | GSM556415
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556415/suppl/GSM556415.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556416 | GPL570 |
|
SSc_133
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_133_2
|
| Sample_geo_accession | GSM556416
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556416/suppl/GSM556416.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556417 | GPL570 |
|
SSc_138
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_138_2
|
| Sample_geo_accession | GSM556417
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556417/suppl/GSM556417.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556418 | GPL570 |
|
SSc_139
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_139_4
|
| Sample_geo_accession | GSM556418
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556418/suppl/GSM556418.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556419 | GPL570 |
|
SSc_140
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_140_4
|
| Sample_geo_accession | GSM556419
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556419/suppl/GSM556419.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556420 | GPL570 |
|
SSc_155
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_155_3
|
| Sample_geo_accession | GSM556420
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556420/suppl/GSM556420.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556421 | GPL570 |
|
SSc_157
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_157_3
|
| Sample_geo_accession | GSM556421
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556421/suppl/GSM556421.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556422 | GPL570 |
|
SSc_158
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma without Pulmonary Hypertension
gender: Female
|
gene expression data
CRN_158_3
|
| Sample_geo_accession | GSM556422
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556422/suppl/GSM556422.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556423 | GPL570 |
|
SSc-PH_48
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_48_1
|
| Sample_geo_accession | GSM556423
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556423/suppl/GSM556423.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556424 | GPL570 |
|
SSc-PH_82
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_82_4
|
| Sample_geo_accession | GSM556424
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556424/suppl/GSM556424.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556425 | GPL570 |
|
SSc-PH_94
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_94_1
|
| Sample_geo_accession | GSM556425
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556425/suppl/GSM556425.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556426 | GPL570 |
|
SSc-PH_114
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_114_3
|
| Sample_geo_accession | GSM556426
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556426/suppl/GSM556426.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556427 | GPL570 |
|
SSc-PH_118
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_118_2
|
| Sample_geo_accession | GSM556427
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556427/suppl/GSM556427.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556428 | GPL570 |
|
SSc-PH_154
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_154_2
|
| Sample_geo_accession | GSM556428
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556428/suppl/GSM556428.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556429 | GPL570 |
|
SSc-PH_163
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_163_6
|
| Sample_geo_accession | GSM556429
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556429/suppl/GSM556429.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556430 | GPL570 |
|
SSc-PH_169
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_169_4
|
| Sample_geo_accession | GSM556430
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556430/suppl/GSM556430.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556431 | GPL570 |
|
SSc-PH_172
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_172_3
|
| Sample_geo_accession | GSM556431
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556431/suppl/GSM556431.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556432 | GPL570 |
|
SSc-PH_174
|
PMBCs
|
cell type: PMBCs
disease state: Scleroderma with Pulmonary Hypertension
gender: Female
|
gene expression data
CRP_174_3
|
| Sample_geo_accession | GSM556432
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556432/suppl/GSM556432.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556433 | GPL570 |
|
IPAH_10
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_10_6
|
| Sample_geo_accession | GSM556433
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556433/suppl/GSM556433.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556434 | GPL570 |
|
IPAH_12
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_12_1
|
| Sample_geo_accession | GSM556434
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556434/suppl/GSM556434.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556435 | GPL570 |
|
IPAH_17
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_17_1
|
| Sample_geo_accession | GSM556435
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556435/suppl/GSM556435.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556436 | GPL570 |
|
IPAH_64
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_64_2
|
| Sample_geo_accession | GSM556436
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556436/suppl/GSM556436.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556437 | GPL570 |
|
IPAH_69
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_69_2
|
| Sample_geo_accession | GSM556437
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556437/suppl/GSM556437.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556438 | GPL570 |
|
IPAH_87
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_87_4
|
| Sample_geo_accession | GSM556438
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556438/suppl/GSM556438.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556439 | GPL570 |
|
IPAH_89
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_89_3
|
| Sample_geo_accession | GSM556439
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556439/suppl/GSM556439.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556440 | GPL570 |
|
IPAH_150
|
PMBCs
|
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
gene expression data
IPAH_150_3
|
| Sample_geo_accession | GSM556440
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556440/suppl/GSM556440.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556441 | GPL570 |
|
NL_2
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_2_1
|
| Sample_geo_accession | GSM556441
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556441/suppl/GSM556441.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556442 | GPL570 |
|
NL_7
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_7_1
|
| Sample_geo_accession | GSM556442
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556442/suppl/GSM556442.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556443 | GPL570 |
|
NL_18
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_18_2
|
| Sample_geo_accession | GSM556443
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556443/suppl/GSM556443.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556444 | GPL570 |
|
NL_23
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_23_2
|
| Sample_geo_accession | GSM556444
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556444/suppl/GSM556444.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556445 | GPL570 |
|
NL_24
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_24_4
|
| Sample_geo_accession | GSM556445
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556445/suppl/GSM556445.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556446 | GPL570 |
|
NL_35
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_35_4
|
| Sample_geo_accession | GSM556446
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556446/suppl/GSM556446.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556447 | GPL570 |
|
NL_53
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_53_4
|
| Sample_geo_accession | GSM556447
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556447/suppl/GSM556447.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556448 | GPL570 |
|
NL_58
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_58_6
|
| Sample_geo_accession | GSM556448
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556448/suppl/GSM556448.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556449 | GPL570 |
|
NL_59
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_59_3
|
| Sample_geo_accession | GSM556449
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556449/suppl/GSM556449.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
GSM556450 | GPL570 |
|
NL_171
|
PMBCs
|
cell type: PMBCs
disease state: Normal (no pulmonary hypertension)
gender: Female
|
gene expression data
NL_171_3
|
| Sample_geo_accession | GSM556450
| | Sample_status | Public on Aug 30 2010
| | Sample_submission_date | Jun 15 2010
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
| | Sample_extract_protocol_ch1 | Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Raw data was normalized using RMA and presented as non-log transformed signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,D,Coldren
| | Sample_contact_email | Chris.Coldren@ucdenver.edu
| | Sample_contact_phone | 303 724 6056
| | Sample_contact_laboratory | Pulmonary Sciences and Critical Care Medicine
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Colorado School of Medicine
| | Sample_contact_address | 12700 East 17th Place
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM556nnn/GSM556450/suppl/GSM556450.CEL.gz
| | Sample_series_id | GSE22356
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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