Result

Search results for the GEO ID: GSE22434

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GSM ID

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Title

Source name

Description

Characteristics

GSM557504

GPL1261

mock-1

5-FU-primed bone marrow mononuclear cells, control transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: mock retroviral transduction

gene expression data from mock-transduced bone marrow

Sample_geo_accession	GSM557504
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557504/suppl/GSM557504.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557505

GPL1261

mock-2

5-FU-primed bone marrow mononuclear cells, control transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: mock retroviral transduction

gene expression data from mock-transduced bone marrow

Sample_geo_accession	GSM557505
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557505/suppl/GSM557505.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557506

GPL1261

mock-3

5-FU-primed bone marrow mononuclear cells, control transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: mock retroviral transduction

gene expression data from mock-transduced bone marrow

Sample_geo_accession	GSM557506
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557506/suppl/GSM557506.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557507

GPL1261

mock-4

5-FU-primed bone marrow mononuclear cells, control transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: mock retroviral transduction

gene expression data from mock-transduced bone marrow

Sample_geo_accession	GSM557507
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557507/suppl/GSM557507.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557508

GPL1261

Evi1-1

5-FU-primed bone marrow mononuclear cells, Evi1-transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: Evi1 retroviral transduction

gene expression data from Evi1-transduced bone marrow

Sample_geo_accession	GSM557508
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557508/suppl/GSM557508.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557509

GPL1261

Evi1-2

5-FU-primed bone marrow mononuclear cells, Evi1-transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: Evi1 retroviral transduction

gene expression data from Evi1-transduced bone marrow

Sample_geo_accession	GSM557509
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557509/suppl/GSM557509.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557510

GPL1261

Evi1-3

5-FU-primed bone marrow mononuclear cells, Evi1-transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: Evi1 retroviral transduction

gene expression data from Evi1-transduced bone marrow

Sample_geo_accession	GSM557510
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557510/suppl/GSM557510.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

GSM557511

GPL1261

Evi1-4

5-FU-primed bone marrow mononuclear cells, Evi1-transduction

strain: C57BL/6 cell type: mononuclear bone marrow cells treatment protocol: Evi1 retroviral transduction

gene expression data from Evi1-transduced bone marrow

Sample_geo_accession	GSM557511
Sample_status	Public on Jun 18 2010
Sample_submission_date	Jun 18 2010
Sample_last_update_date	Jun 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP and cultured in Methocult 3434 for 48 hrs. GFP positive cells were sorted and subjected to the analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNease mini kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of fragmented cDNA was carried out using NuGen Ovation Biotin labeling system.
Sample_hyb_protocol	Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer.
Sample_scan_protocol	Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
Sample_data_processing	Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
Sample_data_processing	Background subtraction, normalization and expression values of our data were calculated using the rma algorithm, available as a part of the Affymetrix package of the Bioconductor open-source software library for the statistical language R (http://www.bioconductor.org).
Sample_platform_id	GPL1261
Sample_contact_name	Akihide,,Yoshimi
Sample_contact_email	ayoshimi-tky@umin.ac.jp
Sample_contact_laboratory	8th lab
Sample_contact_department	Department of Hematology & Oncology
Sample_contact_institute	Tokyo University
Sample_contact_address	7-3-1 Hongo, Bunkyo-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-8655
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557511/suppl/GSM557511.CEL.gz
Sample_series_id	GSE22434
Sample_data_row_count	45101

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