Search results for the GEO ID: GSE22445  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM557643 | GPL96 |
|
untreated 8h rep 1
|
untreated 8h
|
cell line: MSTO-211H
agent: untreated
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557643
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557643/suppl/GSM557643.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557644 | GPL96 |
|
untreated 8h rep 3
|
untreated 8h
|
cell line: MSTO-211H
agent: untreated
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557644
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557644/suppl/GSM557644.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557645 | GPL96 |
|
piroxicam 8h rep 1
|
Piroxicam 8h
|
cell line: MSTO-211H
agent: Piroxicam
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557645
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557645/suppl/GSM557645.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557646 | GPL96 |
|
piroxicam 8h rep 3
|
Piroxicam 8h
|
cell line: MSTO-211H
agent: Piroxicam
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557646
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557646/suppl/GSM557646.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557647 | GPL96 |
|
cisplatin 8h rep 1
|
Cisplatin 8h
|
cell line: MSTO-211H
agent: Cisplatin
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557647
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557647/suppl/GSM557647.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557648 | GPL96 |
|
cisplatin 8h rep 3
|
Cisplatin 8h
|
cell line: MSTO-211H
agent: Cisplatin
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557648
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557648/suppl/GSM557648.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557649 | GPL96 |
|
piroxicam/cisplatin 8h rep 1
|
Piroxicam/Cisplatin 8h
|
cell line: MSTO-211H
agent: Piroxicam/Cisplatin
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557649
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557649/suppl/GSM557649.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557650 | GPL96 |
|
piroxicam/cisplatin 8h rep 3
|
Piroxicam/Cisplatin 8h
|
cell line: MSTO-211H
agent: Piroxicam/Cisplatin
time point: 8h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557650
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557650/suppl/GSM557650.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557651 | GPL96 |
|
untreated 24h rep 1
|
untreated 24h
|
cell line: MSTO-211H
agent: untreated
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557651
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557651/suppl/GSM557651.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557652 | GPL96 |
|
untreated 24h rep 2
|
untreated 24h
|
cell line: MSTO-211H
agent: untreated
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557652
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557652/suppl/GSM557652.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557653 | GPL96 |
|
piroxicam 24h rep 1
|
Piroxicam 24h
|
cell line: MSTO-211H
agent: Piroxicam
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557653
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557653/suppl/GSM557653.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557654 | GPL96 |
|
piroxicam 24h rep 2
|
Piroxicam 24h
|
cell line: MSTO-211H
agent: Piroxicam
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557654
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557654/suppl/GSM557654.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557655 | GPL96 |
|
piroxicam 24h rep 3
|
Piroxicam 24h
|
cell line: MSTO-211H
agent: Piroxicam
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557655
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557655/suppl/GSM557655.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557656 | GPL96 |
|
cisplatin 24h rep 1
|
Cisplatin 24h
|
cell line: MSTO-211H
agent: Cisplatin
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557656
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557656/suppl/GSM557656.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557657 | GPL96 |
|
cisplatin 24h rep 2
|
Cisplatin 24h
|
cell line: MSTO-211H
agent: Cisplatin
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557657
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557657/suppl/GSM557657.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557658 | GPL96 |
|
cisplatin 24h rep 3
|
Cisplatin 24h
|
cell line: MSTO-211H
agent: Cisplatin
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557658
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557658/suppl/GSM557658.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557659 | GPL96 |
|
piroxicam/cisplatin 24h rep 1
|
Piroxicam/Cisplatin 24h
|
cell line: MSTO-211H
agent: Piroxicam/Cisplatin
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557659
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557659/suppl/GSM557659.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
GSM557660 | GPL96 |
|
piroxicam/cisplatin 24h rep 3
|
Piroxicam/Cisplatin 24h
|
cell line: MSTO-211H
agent: Piroxicam/Cisplatin
time point: 24h
cell type: mesothelioma cell
|
-
|
| Sample_geo_accession | GSM557660
| | Sample_status | Public on Nov 20 2011
| | Sample_submission_date | Jun 18 2010
| | Sample_last_update_date | Nov 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | drug treatments, cells were seeded in complete growth medium 16 hours before the experiments, in order to allow attachment but not cell-doubling. Then cells were treated with piroxicam (760 microM) and cisplatin (4.5 microg/mL) alone or in combination for 8 or 24 hours. Controls samples were untreated.
| | Sample_growth_protocol_ch1 | Cells were cultured as monolayers in flasks using American Type Culture Collection complete growth medium in a humidified atmosphere containing 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Midi kit (Qiagen, Valencia, CA, USA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA target preparation was done using Affymetrix kit and following manufacturer indications
| | Sample_hyb_protocol | Hybridization was done using Affymetrix kit and following manufacturer indications
| | Sample_scan_protocol | scanner procedures were done using a Genechip Affymetrix station (FS 450, Scanner 3000) following manufacturer indications
| | Sample_data_processing | Microarray quality control and statistical validation were performed using oneChannelGUI Bioconductor package. Intensity summarization was done with GCRMA and quantile normalization
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raffaele,A,Calogero
| | Sample_contact_email | raffaele.calogero@unito.it
| | Sample_contact_phone | ++39 0116706457
| | Sample_contact_laboratory | Bioinformatics and Genomics Unit
| | Sample_contact_department | Molecular Biotechnology Center
| | Sample_contact_institute | University of Torino
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_state | To
| | Sample_contact_zip/postal_code | 10126
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM557nnn/GSM557660/suppl/GSM557660.CEL.gz
| | Sample_series_id | GSE22445
| | Sample_data_row_count | 22283
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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