Search results for the GEO ID: GSE22506  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM558995 | GPL1261 |
|
White adipose, mouse injected ip. with saline 1
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Gonadal white adipose tissue
|
Gene expression data from white adipose
|
| Sample_geo_accession | GSM558995
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM558nnn/GSM558995/suppl/GSM558995.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM558996 | GPL1261 |
|
White adipose, mouse injected ip. with saline 2
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Gonadal white adipose tissue
|
Gene expression data from white adipose
|
| Sample_geo_accession | GSM558996
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM558nnn/GSM558996/suppl/GSM558996.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM558997 | GPL1261 |
|
White adipose, mouse injected ip. with UAG 1
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Gonadal white adipose tissue
|
Gene expression data from white adipose
|
| Sample_geo_accession | GSM558997
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM558nnn/GSM558997/suppl/GSM558997.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM558998 | GPL1261 |
|
White adipose, mouse injected ip. with UAG 2
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Gonadal white adipose tissue
|
Gene expression data from white adipose
|
| Sample_geo_accession | GSM558998
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM558nnn/GSM558998/suppl/GSM558998.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM558999 | GPL1261 |
|
Muscle, mouse injected ip. with saline 1
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Skeletal muscle (M. vastus lateralis)
|
Gene expression data from skeletal muscle
|
| Sample_geo_accession | GSM558999
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM558nnn/GSM558999/suppl/GSM558999.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559000 | GPL1261 |
|
Muscle, mouse injected ip. with saline 2
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Skeletal muscle (M. vastus lateralis)
|
Gene expression data from skeletal muscle
|
| Sample_geo_accession | GSM559000
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559000/suppl/GSM559000.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559001 | GPL1261 |
|
Muscle, mouse injected ip. with UAG 1
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Skeletal muscle (M. vastus lateralis)
|
Gene expression data from skeletal muscle
|
| Sample_geo_accession | GSM559001
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559001/suppl/GSM559001.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559002 | GPL1261 |
|
Muscle, mouse injected ip. with UAG 2
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Skeletal muscle (M. vastus lateralis)
|
Gene expression data from skeletal muscle
|
| Sample_geo_accession | GSM559002
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559002/suppl/GSM559002.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559003 | GPL1261 |
|
Liver, mouse injected ip. with saline 1
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Liver
|
Gene expression data from liver
|
| Sample_geo_accession | GSM559003
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559003/suppl/GSM559003.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559004 | GPL1261 |
|
Liver, mouse injected ip. with saline 2
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Liver
|
Gene expression data from liver
|
| Sample_geo_accession | GSM559004
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559004/suppl/GSM559004.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559005 | GPL1261 |
|
Liver, mouse injected ip. with UAG 1
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Liver
|
Gene expression data from liver
|
| Sample_geo_accession | GSM559005
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559005/suppl/GSM559005.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
| | |
|
GSM559006 | GPL1261 |
|
Liver, mouse injected ip. with UAG 2
|
GHSR KO mouse (12 weeks)
|
strain: GHSR KO mouse (C57bl/6)
tissue: Liver
|
Gene expression data from liver
|
| Sample_geo_accession | GSM559006
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 22 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then further purified over a Qiagen Rneasy column. RNA was checked for integrity using an Agilent BioAnalyzer and the RNA 6000 Nano Labchip (RIN>=8).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix one cycle protocol using 5 ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G, enabled for high-resolution scanning.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Array intensities and calls were collected using R, and quantile normalization was used on one time present calls. An intensity threshold of 30 was then applied to generate datasets for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patric,,Delhanty
| | Sample_contact_email | p.delhanty@erasmusmc.nl
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559006/suppl/GSM559006.CEL.gz
| | Sample_series_id | GSE22506
| | Sample_data_row_count | 45101
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