Search results for the GEO ID: GSE22527  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM559365 | GPL1261 |
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antiCD3_BDCRAGkoTreg #1
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T cells were sorted from the spleen of BDC2.5 RAGo/o NOD after anti-CD3 treatment
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strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1o/o Foxp3GFP NOD
age: 29 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: anti-CD3 treatment
time: 12 days (after treatment)
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jos4616.CEL
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| Sample_geo_accession | GSM559365
| | Sample_status | Public on Jun 23 2010
| | Sample_submission_date | Jun 23 2010
| | Sample_last_update_date | Jun 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin
| | Sample_hyb_protocol | Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
| | Sample_scan_protocol | After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
| | Sample_data_processing | Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | CBDM,,Lab
| | Sample_contact_email | cbdm@hms.harvard.edu
| | Sample_contact_phone | 617-432-7747
| | Sample_contact_laboratory | CBDM
| | Sample_contact_department | Microbiology and Immunobiology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559365/suppl/GSM559365.CEL.gz
| | Sample_series_id | GSE22527
| | Sample_data_row_count | 45101
| | |
|
GSM559366 | GPL1261 |
|
antiCD3_BDCRAGkoTreg #2
|
T cells were sorted from the spleen of BDC2.5 RAGo/o NOD after anti-CD3 treatment
|
strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1o/o Foxp3GFP NOD
age: 29 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: anti-CD3 treatment
time: 12 days (after treatment)
|
jos4622.CEL
|
| Sample_geo_accession | GSM559366
| | Sample_status | Public on Jun 23 2010
| | Sample_submission_date | Jun 23 2010
| | Sample_last_update_date | Jun 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin
| | Sample_hyb_protocol | Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
| | Sample_scan_protocol | After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
| | Sample_data_processing | Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | CBDM,,Lab
| | Sample_contact_email | cbdm@hms.harvard.edu
| | Sample_contact_phone | 617-432-7747
| | Sample_contact_laboratory | CBDM
| | Sample_contact_department | Microbiology and Immunobiology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559366/suppl/GSM559366.CEL.gz
| | Sample_series_id | GSE22527
| | Sample_data_row_count | 45101
| | |
|
GSM559367 | GPL1261 |
|
antiCD3_BDCRAGkoTreg #3
|
T cells were sorted from the spleen of BDC2.5 RAGo/o NOD after anti-CD3 treatment
|
strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1o/o Foxp3GFP NOD
age: 28 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: anti-CD3 treatment
time: 11 days (after treatment)
|
jos4688.CEL
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| Sample_geo_accession | GSM559367
| | Sample_status | Public on Jun 23 2010
| | Sample_submission_date | Jun 23 2010
| | Sample_last_update_date | Jun 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin
| | Sample_hyb_protocol | Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
| | Sample_scan_protocol | After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
| | Sample_data_processing | Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | CBDM,,Lab
| | Sample_contact_email | cbdm@hms.harvard.edu
| | Sample_contact_phone | 617-432-7747
| | Sample_contact_laboratory | CBDM
| | Sample_contact_department | Microbiology and Immunobiology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559367/suppl/GSM559367.CEL.gz
| | Sample_series_id | GSE22527
| | Sample_data_row_count | 45101
| | |
|
GSM559368 | GPL1261 |
|
BDC Treg #1
|
T cells were sorted from the spleen of BDC2.5 RAG+/o NOD without treatment
|
strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1+/o Foxp3GFP NOD
age: 29 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: no treatment
|
jos4617.CEL
|
| Sample_geo_accession | GSM559368
| | Sample_status | Public on Jun 23 2010
| | Sample_submission_date | Jun 23 2010
| | Sample_last_update_date | Jun 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin
| | Sample_hyb_protocol | Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
| | Sample_scan_protocol | After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
| | Sample_data_processing | Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | CBDM,,Lab
| | Sample_contact_email | cbdm@hms.harvard.edu
| | Sample_contact_phone | 617-432-7747
| | Sample_contact_laboratory | CBDM
| | Sample_contact_department | Microbiology and Immunobiology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559368/suppl/GSM559368.CEL.gz
| | Sample_series_id | GSE22527
| | Sample_data_row_count | 45101
| | |
|
GSM559369 | GPL1261 |
|
BDC Treg #2
|
T cells were sorted from the spleen of BDC2.5 RAG+/o NOD without treatment
|
strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1+/o Foxp3GFP NOD
age: 28 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: no treatment
|
jos4621.CEL
|
| Sample_geo_accession | GSM559369
| | Sample_status | Public on Jun 23 2010
| | Sample_submission_date | Jun 23 2010
| | Sample_last_update_date | Jun 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin
| | Sample_hyb_protocol | Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
| | Sample_scan_protocol | After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
| | Sample_data_processing | Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | CBDM,,Lab
| | Sample_contact_email | cbdm@hms.harvard.edu
| | Sample_contact_phone | 617-432-7747
| | Sample_contact_laboratory | CBDM
| | Sample_contact_department | Microbiology and Immunobiology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559369/suppl/GSM559369.CEL.gz
| | Sample_series_id | GSE22527
| | Sample_data_row_count | 45101
| | |
|
GSM559370 | GPL1261 |
|
BDC Treg #3
|
T cells were sorted from the spleen of BDC2.5 RAG+/o NOD without treatment
|
strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1+/o Foxp3GFP NOD
age: 28 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: no treatment
|
jos4687.CEL
|
| Sample_geo_accession | GSM559370
| | Sample_status | Public on Jun 23 2010
| | Sample_submission_date | Jun 23 2010
| | Sample_last_update_date | Jun 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin
| | Sample_hyb_protocol | Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
| | Sample_scan_protocol | After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
| | Sample_data_processing | Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | CBDM,,Lab
| | Sample_contact_email | cbdm@hms.harvard.edu
| | Sample_contact_phone | 617-432-7747
| | Sample_contact_laboratory | CBDM
| | Sample_contact_department | Microbiology and Immunobiology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559370/suppl/GSM559370.CEL.gz
| | Sample_series_id | GSE22527
| | Sample_data_row_count | 45101
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