Sample_geo_accession | GSM559519
|
Sample_status | Public on Dec 25 2010
|
Sample_submission_date | Jun 23 2010
|
Sample_last_update_date | Dec 25 2010
|
Sample_type | RNA
|
Sample_channel_count | 1
|
Sample_organism_ch1 | Mus musculus
|
Sample_taxid_ch1 | 10090
|
Sample_treatment_protocol_ch1 = Mice were infected with Ad-control (empty adenovirus vector) or Ad-PDK4 (PDK4 overexpressing adenovirus vector) at a dose of 10^9 PFU/mouse by tail vein injection. 3 days after the infection, mice were sacrificed for RNA preparation from the liver. Ad-control infected = 4, Ad-PDK4 infected | 3. Specimens from mice of each group were pooled, and 10 ug RNA from each pool was used for cRNA synthesis.
|
Sample_growth_protocol_ch1 | Mice were fed in isolate chamber maintained at 23C with a cycle of 12 h light and shade.
|
Sample_molecule_ch1 | total RNA
|
Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy mini kit (Qiagen). The RNA integrity number (RIN) was determined using an Agilent Technologies 2100 bioanalyzer and A2100 expert software (ver. B0202SI238; Agilent).
|
Sample_label_ch1 | biotin
|
Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 μg total RNA
|
Sample_hyb_protocol | Following fragmentation, the synthesized cRNA was hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Arrays. The arrays were washed and stained using an Affymetrix Fluidics Station 450
|
Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
Sample_data_processing | Cel files were analyzed with Affymetrix GeneChip Operating Software (version 1.3). All probe sets were scaled to a target signal of 500.
|
Sample_platform_id | GPL1261
|
Sample_contact_name | Minori,,Dateki
|
Sample_contact_email | mdateki@nifty.com
|
Sample_contact_department | Environmental Science Laboratory
|
Sample_contact_institute | Central Research Institute of Electric Power Industry
|
Sample_contact_address | 1646
|
Sample_contact_city | Abiko
|
Sample_contact_state | Chiba
|
Sample_contact_zip/postal_code | 270-1194
|
Sample_contact_country | Japan
|
Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM559nnn/GSM559519/suppl/GSM559519.CEL.gz
|
Sample_series_id | GSE22534
|
Sample_data_row_count | 45101
|
|