Search results for the GEO ID: GSE22580  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM560337 | GPL570 |
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Type I hTERT-immortalized hMECs
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human mammary epithelial cells (TERT immortalized), K5+K19-
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gender: female
tissue: breast
cell type: hTERT-immortalized K5+K19- mammary epithelial cells
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K5+/K19-
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| Sample_geo_accession | GSM560337
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 25 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | No treatment. Logarithmically growing cells were collected for RNA extraction.
| | Sample_growth_protocol_ch1 | Normal human mammary epithelial cells (hMECs) were isolated from Reduction Mammoplasty. hTERT-immortalized hMEC stem/progenitor and myoepithelial cells were grown in DFCI-1 and MEGM medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express protocol from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gautam,,Malhotra
| | Sample_contact_institute | UNMC
| | Sample_contact_address | 985805 Nebraska Medical Center
| | Sample_contact_city | Omaha
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68198-5805
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560337/suppl/GSM560337.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560337/suppl/GSM560337.CHP.gz
| | Sample_series_id | GSE22580
| | Sample_data_row_count | 54675
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GSM560338 | GPL570 |
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Type II hTERT-immortalized hMECs
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human mammary epithelial cells (TERT immortalized), K5+K19+
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gender: female
tissue: breast
cell type: hTERT-immortalized K5+K19+ mammary epithelial cells
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K5+/K19+
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| Sample_geo_accession | GSM560338
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 25 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | No treatment. Logarithmically growing cells were collected for RNA extraction.
| | Sample_growth_protocol_ch1 | Normal human mammary epithelial cells (hMECs) were isolated from Reduction Mammoplasty. hTERT-immortalized hMEC stem/progenitor and myoepithelial cells were grown in DFCI-1 and MEGM medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express protocol from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gautam,,Malhotra
| | Sample_contact_institute | UNMC
| | Sample_contact_address | 985805 Nebraska Medical Center
| | Sample_contact_city | Omaha
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68198-5805
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560338/suppl/GSM560338.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560338/suppl/GSM560338.CHP.gz
| | Sample_series_id | GSE22580
| | Sample_data_row_count | 54675
| | |
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GSM560339 | GPL570 |
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Myoepithelial cells
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myoepithelial cells differentiated from human mammary epithelial cells (TERT immortalized) K5+K19-
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gender: female
tissue: breast
cell type: myoepithelial cells differentiated from hTERT-immortalized K5+K19- mammary epithelial cells
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α-SMA+/Thy1+/CD10+
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| Sample_geo_accession | GSM560339
| | Sample_status | Public on Jun 26 2010
| | Sample_submission_date | Jun 25 2010
| | Sample_last_update_date | Jun 25 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | No treatment. Logarithmically growing cells were collected for RNA extraction.
| | Sample_growth_protocol_ch1 | Normal human mammary epithelial cells (hMECs) were isolated from Reduction Mammoplasty. hTERT-immortalized hMEC stem/progenitor and myoepithelial cells were grown in DFCI-1 and MEGM medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express protocol from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gautam,,Malhotra
| | Sample_contact_institute | UNMC
| | Sample_contact_address | 985805 Nebraska Medical Center
| | Sample_contact_city | Omaha
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68198-5805
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560339/suppl/GSM560339.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560339/suppl/GSM560339.CHP.gz
| | Sample_series_id | GSE22580
| | Sample_data_row_count | 54675
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