Search results for the GEO ID: GSE22593  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM560575 | GPL96 |
|
WT-0 Hr E2_100A
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 0 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560575
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560575/suppl/GSM560575.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560576 | GPL96 |
|
WT-0 Hr E2_100B
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 0 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560576
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560576/suppl/GSM560576.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560577 | GPL96 |
|
WT-1 Hr E2_101A
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 1 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560577
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560577/suppl/GSM560577.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560578 | GPL96 |
|
WT-1 Hr E2_101B
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 1 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560578
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560578/suppl/GSM560578.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560579 | GPL96 |
|
WT-2 Hr E2_102A
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 2 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560579
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560579/suppl/GSM560579.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560580 | GPL96 |
|
WT-2 Hr E2_102B
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 2 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560580
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560580/suppl/GSM560580.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560581 | GPL96 |
|
WT-4 Hr E2_103A
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 4 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560581
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560581/suppl/GSM560581.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560582 | GPL96 |
|
WT-4 Hr E2_103B
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 4 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560582
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560582/suppl/GSM560582.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560583 | GPL96 |
|
WT-8 Hr E2_104A
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 8 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560583
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560583/suppl/GSM560583.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560584 | GPL96 |
|
WT-8 Hr E2_104B
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 8 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560584
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560584/suppl/GSM560584.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560585 | GPL96 |
|
WT-24 Hr E2_105A
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 24 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560585
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560585/suppl/GSM560585.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560586 | GPL96 |
|
WT-24 Hr E2_105B
|
MDA-MB-231 cells
|
genotype/variation: WT-ER
time point: 24 Hr E2
|
Breast Cancer Cells Stably expressing Estrogen Receptor
|
| Sample_geo_accession | GSM560586
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560586/suppl/GSM560586.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560587 | GPL96 |
|
DBDmut-0 Hr E2_106A2
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 0 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560587
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560587/suppl/GSM560587.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560588 | GPL96 |
|
DBDmut-0 Hr E2_106B
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 0 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560588
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560588/suppl/GSM560588.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560589 | GPL96 |
|
DBDmut-1 Hr E2_107A
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 1 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560589
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560589/suppl/GSM560589.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560590 | GPL96 |
|
DBDmut-1 Hr E2_107B
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 1 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560590
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560590/suppl/GSM560590.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560591 | GPL96 |
|
DBDmut-2 Hr E2_108A
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 2 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560591
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560591/suppl/GSM560591.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560592 | GPL96 |
|
DBDmut-2 Hr E2_108B
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 2 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560592
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560592/suppl/GSM560592.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560593 | GPL96 |
|
DBDmut-4 Hr E2_109A
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 4 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560593
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560593/suppl/GSM560593.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560594 | GPL96 |
|
DBDmut-4 Hr E2_109B
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 4 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560594
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560594/suppl/GSM560594.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560595 | GPL96 |
|
DBDmut-8 Hr E2_110A
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 8 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560595
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560595/suppl/GSM560595.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560596 | GPL96 |
|
DBDmut-8 Hr E2_110B
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 8 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560596
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560596/suppl/GSM560596.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560597 | GPL96 |
|
DBDmut-24 Hr E2_111A
|
MDA-MB-231 cells
|
genotype/variation: DBDmut-ER
time point: 24 Hr E2
|
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560597
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560597/suppl/GSM560597.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
|
GSM560598 | GPL96 |
|
DBDmut-24 Hr E2_111B
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MDA-MB-231 cells
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genotype/variation: DBDmut-ER
time point: 24 Hr E2
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Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Sample_geo_accession | GSM560598
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jun 28 2010
| | Sample_last_update_date | Jun 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 10 nM E2 for 1-24 hours.
| | Sample_growth_protocol_ch1 | Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
| | Sample_platform_id | GPL96
| | Sample_contact_name | Joshua,D,Stender
| | Sample_contact_institute | University of California at San Diego
| | Sample_contact_address | 9500 Gillman
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM560nnn/GSM560598/suppl/GSM560598.CEL.gz
| | Sample_series_id | GSE22593
| | Sample_series_id | GSE22610
| | Sample_data_row_count | 22283
| | |
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