Search results for the GEO ID: GSE22637  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM561283 | GPL1261 |
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Control, EB Day2
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Embryoid bodies formed from hanging drops
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cell line: CGR8 ES cells
strain: 129
agent: none
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Expression data-control-differentiation medium only
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| Sample_geo_accession | GSM561283
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jun 30 2010
| | Sample_last_update_date | Jun 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Embryoid bodies were formed by hanging drops and then suspended in differentiation medium on day 2 with recombinant proteins (100 ng/ml)
| | Sample_growth_protocol_ch1 | ES cells were cultured without feeder cells in GMEM (Glasgow Minimum Essential Medium) supplemented with pyruvate, non-essential amino acids, 2-mercaptoethanol, 10% ES cell fetal bovine serum, and leukemia inhibitory factor (LIF)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from EBs on day 2 by using Trizol reagent (Invitrogen Carlsbad, CA) according to the manufacturer`s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10ug total RNA were used for cDNA synthesis, labeled by in vitro transcription followed by fragmentation following the manufactory’s suggestion (GeneChip Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | 11ug labeled samples were hybridized to GeneChip* at 45℃ for 16.5 hours.
| | Sample_scan_protocol | The wash and staining were performed by Fluidic Station-450 and the the GeneChip* were scanned with Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data was first analyzed, globly normalized, and backup correction with MAS 5.0 provied by Affymetrix and the Scale Factor is 2.798.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Vivienne,HJ,Lee
| | Sample_contact_laboratory | PH lab
| | Sample_contact_department | Institute of Clinical Investigation
| | Sample_contact_institute | National Cheng Kung University
| | Sample_contact_address | No.100, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)
| | Sample_contact_city | Tainan
| | Sample_contact_zip/postal_code | 70101
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM561nnn/GSM561283/suppl/GSM561283.CEL.gz
| | Sample_series_id | GSE22637
| | Sample_data_row_count | 45101
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GSM561284 | GPL1261 |
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FGF-10, EB Day2
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Embryoid bodies formed from hanging drops
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cell line: CGR8 ES cells
strain: 129
agent: 100 ng/ml FGF10
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Expression data-FGF-10-differentiation medium plus FGF-10 recombinant protein (100ng/ml)
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| Sample_geo_accession | GSM561284
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jun 30 2010
| | Sample_last_update_date | Jun 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Embryoid bodies were formed by hanging drops and then suspended in differentiation medium on day 2 with recombinant proteins (100 ng/ml)
| | Sample_growth_protocol_ch1 | ES cells were cultured without feeder cells in GMEM (Glasgow Minimum Essential Medium) supplemented with pyruvate, non-essential amino acids, 2-mercaptoethanol, 10% ES cell fetal bovine serum, and leukemia inhibitory factor (LIF)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from EBs on day 2 by using Trizol reagent (Invitrogen Carlsbad, CA) according to the manufacturer`s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10ug total RNA were used for cDNA synthesis, labeled by in vitro transcription followed by fragmentation following the manufactory’s suggestion (GeneChip Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | 11ug labeled samples were hybridized to GeneChip* at 45℃ for 16.5 hours.
| | Sample_scan_protocol | The wash and staining were performed by Fluidic Station-450 and the the GeneChip* were scanned with Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data was first analyzed, globly normalized, and backup correction with MAS 5.0 provied by Affymetrix and the Scale Factor is 2.798.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Vivienne,HJ,Lee
| | Sample_contact_laboratory | PH lab
| | Sample_contact_department | Institute of Clinical Investigation
| | Sample_contact_institute | National Cheng Kung University
| | Sample_contact_address | No.100, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)
| | Sample_contact_city | Tainan
| | Sample_contact_zip/postal_code | 70101
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM561nnn/GSM561284/suppl/GSM561284.CEL.gz
| | Sample_series_id | GSE22637
| | Sample_data_row_count | 45101
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