Result

Search results for the GEO ID: GSE22791

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GSM ID

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Title

Source name

Description

Characteristics

GSM563495

GPL1355

0 hr

o hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 0 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563495
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563495/suppl/GSM563495.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563495/suppl/GSM563495.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

GSM563496

GPL1355

2 hr

2 hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 2 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563496
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563496/suppl/GSM563496.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563496/suppl/GSM563496.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

GSM563497

GPL1355

6 hr

6 hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 6 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563497
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563497/suppl/GSM563497.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563497/suppl/GSM563497.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

GSM563498

GPL1355

12 hr

12 hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 12 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563498
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563498/suppl/GSM563498.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563498/suppl/GSM563498.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

GSM563499

GPL1355

24 hr

24 hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 24 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563499
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563499/suppl/GSM563499.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563499/suppl/GSM563499.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

GSM563500

GPL1355

48 hr

48 hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 48 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563500
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563500/suppl/GSM563500.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563500/suppl/GSM563500.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

GSM563501

GPL1355

72 hr

72 hr after mechanical stress

strain: Sprague-Dawley age: E15 tissue: cultured spinal cord cells

Gene expression 72 hr after stress in E15 Sprague-Dawley rats spinal cord

Sample_geo_accession	GSM563501
Sample_status	Public on Jul 08 2010
Sample_submission_date	Jul 07 2010
Sample_last_update_date	Jul 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Dissected tissues were rinsed with cold Ca2+ -and Mg2+-free Hanks balanced salt solution (HBSS) supplemented with 4 g/L glucose, and incubated at 37°C for 20 minutes with 0.03% (w/v) trypsin solution in HBSS under mild shaking.
Sample_growth_protocol_ch1	The spinal cords of Sprague-Dawley rat embryos were dissected out at post-coital day 15 and stripped of the dorsal root ganglia and meninges.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cultured cells on each well at 0, 2, 6, 12, 24, 48 and 72 hours were disrupted in a lysis buffer containing β-mercaptoethanol and the total RNA from five animals was pooled at each time point, and further purified using RNeasy® Mini Kit (Qiagen, Valencia, CA) and treated with DNase I (TaKaRa Bio, Ohtsu, Japan)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were synthesized using the IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 µg of cRNA were hybridized for 16 hours at 45°C on the GeneChip® Rat Genome 230 2.0 Array.
Sample_scan_protocol	GeneChips were washed and stained in the Affymetrix Fluidics Station 450, and scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Microarray data was initially processed using GeneChip Operating Software (GCOS, Affymetrix). Signal intensity was calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as the normalization method.
Sample_platform_id	GPL1355
Sample_contact_name	Kenzo,,Uchida
Sample_contact_email	kuchida@u-fukui.ac.jp
Sample_contact_phone	81-776-61-8383
Sample_contact_fax	81-776-61-8125
Sample_contact_department	Department of Orthopaedics and Rehabilitation Medicine
Sample_contact_institute	Fukui University Faculty of Medical Sciences
Sample_contact_address	Shimoaizuki 23
Sample_contact_city	Matsuoka
Sample_contact_state	Fukui
Sample_contact_zip/postal_code	910-1193
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563501/suppl/GSM563501.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM563nnn/GSM563501/suppl/GSM563501.CHP.gz
Sample_series_id	GSE22791
Sample_data_row_count	31099

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