Result

Search results for the GEO ID: GSE22856

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Title

Source name

Description

Characteristics

GSM564815

GPL1261

lung tissues PF68 treated on day 1, biological rep1

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: PF68 treated on day 1

Gene expression data from mice lung tissues treated with PF 68 (as solvent control) in acute response phase, day 1).

Sample_geo_accession	GSM564815
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564815/suppl/GSM564815.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564816

GPL1261

lung tissues PF68 treated on day 1, biological rep2

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: PF68 treated on day 1

Gene expression data from mice lung tissues treated with PF 68 (as solvent control) in acute response phase, day 1).

Sample_geo_accession	GSM564816
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564816/suppl/GSM564816.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564817

GPL1261

lung tissues 0.5mg SWCNTs treated on day 1, biological rep1

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: 0.5mg SWCNTs treated on day 1

Gene expression data from mice lung tissues treated with 0.5 mg SWCNTs in acute response phase, day 1).

Sample_geo_accession	GSM564817
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564817/suppl/GSM564817.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564818

GPL1261

lung tissues 0.5mg SWCNTs treated on day 1, biological rep2

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: 0.5mg SWCNTs treated on day 1

Gene expression data from mice lung tissues treated with 0.5 mg SWCNTs in acute response phase, day 1).

Sample_geo_accession	GSM564818
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564818/suppl/GSM564818.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564819

GPL1261

lung tissues PF68 treated on day 7, biological rep1

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: PF68 treated on day 7

Gene expression data from mice lung tissues treated with PF 68 (as solvent control) in sub-chronic response phase, day 7).

Sample_geo_accession	GSM564819
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564819/suppl/GSM564819.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564820

GPL1261

lung tissues PF68 treated on day 7, biological rep2

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: PF68 treated on day 7

Gene expression data from mice lung tissues treated with PF 68 (as solvent control) in sub-chronic response phase, day 7).

Sample_geo_accession	GSM564820
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564820/suppl/GSM564820.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564821

GPL1261

lung tissues 0.5mg SWCNTs treated on day 7, biological rep1

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: 0.5mg SWCNTs treated on day 7

Gene expression data from mice lung tissues treated with 0.5 mg SWCNTs in sub-chronic response phase, day 7).

Sample_geo_accession	GSM564821
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564821/suppl/GSM564821.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

GSM564822

GPL1261

lung tissues 0.5mg SWCNTs treated on day 7, biological rep2

lung tissue (right side, lobe 1)

tissue: right lung, lobe 1 strain: ICR age: 5 to 6 weeks old treatment group: 0.5mg SWCNTs treated on day 7

Gene expression data from mice lung tissues treated with 0.5 mg SWCNTs in sub-chronic response phase, day 7).

Sample_geo_accession	GSM564822
Sample_status	Public on Jul 09 2011
Sample_submission_date	Jul 09 2010
Sample_last_update_date	Jul 09 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The intratracheal instillation procedure: anesthetized with pentobarbital (80 mg•kg-1, Sigma, St. Louis, MO, USA; intraperitoneally), individual mice were secured on an inclined plastic platform. The trachea was exposed by a 1 cm incision in the ventral neck skin for instillation of Pluronic F-68 (PF68, solvent control) or the SWCNTs [0.5 mg per mouse in a 50 µl 0.1% Pluronic F-68 aliquot]; SWCNTs (SE grade, purity > 95%) was purchased from CarboLex, Inc, (McCarty, Court, Lexington).
Sample_growth_protocol_ch1	Adult male ICR mice (5~6 weeks old), were purchased from the laboratory animal center of National Taiwan University College of Medicine (Taipei, Taiwan). Mice were cared and used humanely according to the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Health Research Institutes (NHRI, Taiwan).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430A plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
Sample_platform_id	GPL1261
Sample_contact_name	Huei-Wen,,Chen
Sample_contact_email	shwchen@ntu.edu.tw
Sample_contact_phone	+886-2-23123456
Sample_contact_fax	+886-2-23958341
Sample_contact_laboratory	Graduate Institute of Toxicology
Sample_contact_department	Medical College
Sample_contact_institute	National Taiwan University
Sample_contact_address	No.1, Sec.1, Re-Ai Rd
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM564nnn/GSM564822/suppl/GSM564822.CEL.gz
Sample_series_id	GSE22856
Sample_data_row_count	45101

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