Search results for the GEO ID: GSE22969  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM567030 | GPL1261 |
|
Wt adult dc_1
|
Dorsal cerebral cortex
|
strain: 129/Sv
gender: Male
genotype/variation: wild type
developmental stage: adult
age (days): 155
tissue: Dorsal cerebral cortex
mouse number: 245
|
affs591-1-190406.CEL
|
| Sample_geo_accession | GSM567030
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567030/suppl/GSM567030.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567031 | GPL1261 |
|
Qkf adult dc_3
|
Dorsal cerebral cortex
|
strain: 129/Sv
gender: Male
genotype/variation: Qkf gt/gt mutant
developmental stage: adult
age (days): 155
tissue: Dorsal cerebral cortex
mouse number: 246
|
affs591-3-190406.CEL
|
| Sample_geo_accession | GSM567031
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567031/suppl/GSM567031.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567032 | GPL1261 |
|
Wt adult dc_2
|
Dorsal cerebral cortex
|
strain: 129/Sv
gender: Male
genotype/variation: wild type
developmental stage: adult
age (days): 112
tissue: Dorsal cerebral cortex
mouse number: 333
|
affs591-2-190406.CEL
|
| Sample_geo_accession | GSM567032
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567032/suppl/GSM567032.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567033 | GPL1261 |
|
Qkf adult dc_4
|
Dorsal cerebral cortex
|
strain: 129/Sv
gender: Male
genotype/variation: Qkf gt/gt mutant
developmental stage: adult
age (days): 112
tissue: Dorsal cerebral cortex
mouse number: 336
|
affs591-4-190406.CEL
|
| Sample_geo_accession | GSM567033
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567033/suppl/GSM567033.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567034 | GPL1261 |
|
Wt adult dc_13
|
Dorsal cerebral cortex
|
strain: 129/Sv
gender: Male
genotype/variation: wild type
developmental stage: adult
age (days): 101
tissue: Dorsal cerebral cortex
mouse number: 400
|
affs591-13-190406.CEL
|
| Sample_geo_accession | GSM567034
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567034/suppl/GSM567034.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567035 | GPL1261 |
|
Qkf adult dc_14
|
Dorsal cerebral cortex
|
strain: 129/Sv
gender: Male
genotype/variation: Qkf gt/gt mutant
developmental stage: adult
age (days): 99
tissue: Dorsal cerebral cortex
mouse number: 402
|
affs591-14-190406.CEL
|
| Sample_geo_accession | GSM567035
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567035/suppl/GSM567035.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567036 | GPL1261 |
|
Wt edt_25
|
Dorsal telencephalon
|
strain: 129/Sv
gender: NA
genotype/variation: wild type
developmental stage: embryo
age (days): E12.5
tissue: Dorsal telencephalon
mouse number: ♀296 x ♂286
|
affs591-25-200406.CEL
|
| Sample_geo_accession | GSM567036
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567036/suppl/GSM567036.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567037 | GPL1261 |
|
Qkf edt_26r
|
Dorsal telencephalon
|
strain: 129/Sv
gender: NA
genotype/variation: Qkf gt/gt mutant
developmental stage: embryo
age (days): E12.5
tissue: Dorsal telencephalon
mouse number: ♀296 x ♂286
|
affs591-26r-270406.CEL
|
| Sample_geo_accession | GSM567037
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567037/suppl/GSM567037.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567038 | GPL1261 |
|
Wt edt_28
|
Dorsal telencephalon
|
strain: 129/Sv
gender: NA
genotype/variation: wild type
developmental stage: embryo
age (days): E12.5
tissue: Dorsal telencephalon
mouse number: ♀288 x ♂283
|
affs591-28-200406.CEL
|
| Sample_geo_accession | GSM567038
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567038/suppl/GSM567038.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567039 | GPL1261 |
|
Qkf edt_27
|
Dorsal telencephalon
|
strain: 129/Sv
gender: NA
genotype/variation: Qkf gt/gt mutant
developmental stage: embryo
age (days): E12.5
tissue: Dorsal telencephalon
mouse number: ♀288 x ♂283
|
affs591-27-200406.CEL
|
| Sample_geo_accession | GSM567039
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567039/suppl/GSM567039.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567040 | GPL1261 |
|
Wt edt_29
|
Dorsal telencephalon
|
strain: 129/Sv
gender: NA
genotype/variation: wild type
developmental stage: embryo
age (days): E12.5
tissue: Dorsal telencephalon
mouse number: ♀288 x ♂283
|
affs591-29-200406.CEL
|
| Sample_geo_accession | GSM567040
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567040/suppl/GSM567040.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
GSM567041 | GPL1261 |
|
Qkf edt_30
|
Dorsal telencephalon
|
strain: 129/Sv
gender: NA
genotype/variation: Qkf gt/gt mutant
developmental stage: embryo
age (days): E12.5
tissue: Dorsal telencephalon
mouse number: ♀288 x ♂283
|
affs591-30-200406.CEL
|
| Sample_geo_accession | GSM567041
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jul 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by preferentially precipitating RNA from an aqueous guanidine hydrochloride solution using the method described by Chirgwin et. al., 1979 (Biochemistry 18:5294-5299)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm (Bioconductor package gcrma).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gordon,K,Smyth
| | Sample_contact_email | smyth@wehi.edu.au
| | Sample_contact_phone | (+61 3) 9345 2326
| | Sample_contact_fax | (+61 3) 9347 0852
| | Sample_contact_laboratory | Smyth
| | Sample_contact_department | Bioinformatics
| | Sample_contact_institute | Walter and Eliza Hall Institute of Medical Research
| | Sample_contact_address | 1G Royal Pde
| | Sample_contact_city | Parkville
| | Sample_contact_state | Vic
| | Sample_contact_zip/postal_code | 3052
| | Sample_contact_country | Australia
| | Sample_contact_web_link | http://www.wehi.edu.au
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567041/suppl/GSM567041.CEL.gz
| | Sample_series_id | GSE22969
| | Sample_data_row_count | 45101
| | |
|
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